LOCUS       BBa_K784043             2831 bp    DNA     linear       22-Nov-2024
DEFINITION  Mutant T7 RNAP - T7*
ACCESSION   BBa_K784043
VERSION     BBa_K784043.1  
FEATURES             Location/Qualifiers
     RBS             23..56
                     /label=RBS
     CDS             57..2831
                     /label=T7*
     misc_feature    58..176
                     /label=umu tag
ORIGIN
        1 ccagcactgt aataggcact aatatccaaa ccagtagctc aattggagtc gtctatgtgt
       61 tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt tccgctattt agcgatcttg
      121 ttcagtgtgg ctttccttca ccggcagcag attacgttga acagcgcatc gatctgggtg
      181 gcaacacgat taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt
      241 tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc
      301 atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag
      361 ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta ctccctaaga
      421 tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga
      481 cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga
      541 ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa
      601 tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc
      661 acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagaaag
      721 catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt ggcgaggcgt
      781 ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc gagatgctca
      841 ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta ggtcaagact
      901 ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc
      961 tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg tggactggca
     1021 ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca
     1081 gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac aaagcgatta
     1141 acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc gccaacgtaa
     1201 tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt gaagaactcc
     1261 cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg
     1321 ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc cttgagttca
     1381 tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct tacaacatgg
     1441 actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca
     1501 aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac tactggctga
     1561 aaatccacgg tgcaaactgt gcgggtgtcg acaaggttcc gttccctgag cgcatcaagt
     1621 tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg gagaacactt
     1681 ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag tacgctgggg
     1741 tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac gggtcttgct
     1801 ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc gcggttaact
     1861 tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa gtcaacgaga
     1921 ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg accgatgaga
     1981 acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct ggtcaatggc
     2041 tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg gcttacgggt
     2101 ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca gctattgatt
     2161 ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg gctaagctga
     2221 tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac tggcttaagt
     2281 ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag attcttcgca
     2341 agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag gaatacaaga
     2401 agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta cagcctacca
     2461 ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt atcgctccta
     2521 actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga
     2581 agtacggaat cgaatctttt gcactgattc acgactcctt cggtacgatt ccggctgacg
     2641 ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg
     2701 tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa ttggacaaaa
     2761 tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag tcggacttcg
     2821 cgttcgcgta a
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