LOCUS       BBa_K784053             2852 bp    DNA     linear       29-Nov-2024
DEFINITION  theophylline riboswitch + T7*(T3) RNAP
ACCESSION   BBa_K784053
VERSION     BBa_K784053.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=Additional 5' UTR
     misc_feature    18..55
                     /label=Theophylline aptamer
     RBS             56..74
                     /label=RBS
     misc_feature    1..74
                     /label=BBa_K784005
     misc_feature    75..194
                     /label=proposed umu tag
     CDS             75..2852
                     /label=T7*(T3) RNAP
     CDS             75..2852
                     /label=BBa_K784052
ORIGIN
        1 gactcactat aggtaccggt gataccagca tcgtcttgat gcccttggca gcaccctgct
       61 aaggtaacaa caagatgttg tttatcaagc ctgcggatct ccgcgaaatt gtgacttttc
      121 cgctatttag cgatcttgtt cagtgtggct ttccttcacc ggcagcagat tacgttgaac
      181 agcgcatcga tctgggtggc aacacgatta acatcgctaa gaacgacttc tctgacatcg
      241 aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg
      301 aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt
      361 ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca
      421 ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta
      481 agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag
      541 cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc
      601 aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc
      661 gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag
      721 ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc
      781 tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac
      841 gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg
      901 gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg
      961 caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc
     1021 ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg
     1081 cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg
     1141 aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc
     1201 tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga
     1261 ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca
     1321 ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc
     1381 gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct
     1441 ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc
     1501 aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg
     1561 aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgac aaggttccgt
     1621 tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt
     1681 ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct
     1741 gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg
     1801 cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag
     1861 gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg
     1921 ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag
     1981 ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg
     2041 cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca
     2101 tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca
     2161 ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg
     2221 gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag
     2281 caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga
     2341 ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg
     2401 tgtggcagga atacaagaag cctattcaga agcgcctgga catgattttc ttgggtcaat
     2461 ttcgcttgca acctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg
     2521 agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg
     2581 tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg
     2641 gtacgattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca
     2701 catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg
     2761 agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca
     2821 tcttagagtc ggacttcgcg ttcgcgtaat aa
//