BBa_K785000
1
Lov-tetR
Lov-tetR (light sensor with Ptet repression) light->PtetO repressed
2012-09-20T11:00:00Z
2015-05-08T01:13:22Z
This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.
Released HQ 2013
This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light.
This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA.
We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.
false
false
_1040_
0
11000
9
In stock
false
This part is designed for our biowave project.
false
Zining Hou
annotation2194042
1
linker part
range2194042
1
213
345
annotation2194041
1
Lov light sensor domain
range2194041
1
1
294
annotation2194043
1
tetR DNA binding domain
range2194043
1
294
1044
BBa_K785000_sequence
1
atgggtgaatttctggcaaccaccctggaacgtattgaaaaaaactttgttattaccgatccgcgtctgccggataatccgatcatttttgcaagcgatagctttctgcagctgaccgaatatagccgtgaagaaattctgggtcgtaattgtcgttttctgcagggtccggaaaccgatcgtgcaaccgttcgtaaaattcgtgatgcaattgataatcagaccgaagttaccgttcagctgatcaattataccaaaagcggcaaaaaattctggaacctgtttcatctgcagccgatgcgtgatcagaaaggtgatgttcagtattttatcggtgttcagctggatggcaccgaacatgtgcgtgatgcagcagaacgtgaaggtgttatgctgatcaaaaaaaccgcagaaaacattgatgaagcagccaatcgtgaaagcgttattgatgcagccctggaactgctgaatgaaaccggtattgatggtctgaccacccgtaaactggcacagaaactgggtattgaacagccgaccctgtattggcatgttaaaaacaaacgtgcactgctggatgcactggcagttgaaatcctggcacgtcatcatgattattcactgcctgcagccggtgaaagctggcagtcatttctgcgtaataatgcaatgagctttcgtcgtgccctgctgcgttatcgtgatggtgcaaaagttcatctgggcacccgtccggatgaaaaacagtatgataccgttgaaacccagctgcgttttatgaccgaaaatggttttagcctgcgtgatggcctgtatgcaattagcgcagttagccattttaccctgggtgcagttctggaacagcaagaacataccgcagcactgaccgatcgtccggcagcacctgatgaaaatctgcctccgctgctgcgtgaagcactgcagattatggatagtgatgatggtgaacaggcatttctgcatggcctggaaagcctgattcgtggttttgaagttcaactgaccgcactgctgcagtgataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z