BBa_K786001 1 BBa_K786001 Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light 2012-09-20T11:00:00Z 2015-05-08T01:13:22Z From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12. Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression. false false _1041_ 0 14285 9 It's complicated false Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII. false Leung Wai Tak annotation2199668 1 linker range2199668 1 785 811 annotation2199666 1 HindIII for N-terminal fusin range2199666 1 62 67 annotation2199669 1 BamHI for C-terminal fusion range2199669 1 785 790 annotation2199664 1 Constitutive promoter BBa_J23100 range2199664 1 1 42 annotation2199670 1 HtrII range2199670 1 812 1210 annotation2199665 1 RBS 1.0 range2199665 1 43 55 annotation2199672 1 B0015 range2199672 1 2105 2233 annotation2199667 1 Sensory Rhodopsin II range2199667 1 68 784 annotation2199671 1 Tsr range2199671 1 1210 2070 BBa_K786001_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagtgaaagaggagaaatactagaagcttatggtgggacttacgaccctcttttggctcggcgcaatcggcatgctcgtcggcacgctcgcgttcgcgtgggccggccgtgacgccggaagcggcgagcgacggtactacgtgacacttgtcggcatcagtggtatcgcagcagtcgcctacgccgttatggcgctgggtgtcggctgggttcccgtggccgaacggactgttttcgtcccccggtacatcgactggattctcacaaccccgctcatcgtctacttcctcgggctgcttgcggggcttgatagtcgggagttcggcatcgtcatcacgctcaacaccgtggtcatgctcgccggcttcgccggggcgatggtgcccggtatcgagcgctacgcgctgttcggcatgggggcggtcgcattcatcggactggtctactacctcgtcgggccgatgaccgaaagcgccagccagcggtcctccggaatcaagtcgctgtacgtccgcctccgaaacctgacggtcgtcctctgggcgatttatccgttcatctggctgcttggaccgccgggcgtggcgctgctgacaccgactgtcgacgtggcgcttatcgtctaccttgacctcgtcacgaaggtcgggttcggcttcatcgcactcgatgctgcggcgacacttcgggccgaacacggcgaatcgctcgctggcgtcgatactgacacgcctgcggtcgccgacggatccgcgtcgaacggcgcgtcggcgatgtcgctgaacgtatcacggctccttctccccggccgtgtccggcacagttatacggggaaaatgggtgccaccttcgcctttgtcggcgcgttaacggtgctcttcggagccatcgcatacggtgaggtaaccgccgccgccgcgaccggcgatgccgcagccgtacaggaggcggcagtatcggccattctcgggctcatcatcctgctcgggatcaacctcgggctcgttgctgccacgctgggcggtgacaccgccgcctcgctttcaacgctggccgcgaaggcctcgcggatgggcgacggcgacctcgatgtcgagcttgagacccgtcgcgaggacgaaatcggcgacctctatgcggccttcgacgagatgcgccagtcggtgcggacaaccgtcggtgatgtgcgcaacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z