BBa_K786002
1
BBa_K786002
Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
2012-09-20T11:00:00Z
2015-05-08T01:13:22Z
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
Released HQ 2013
Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm.
We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
false
false
_1041_
0
14285
9
In stock
true
Pfam (version 26.0) was used to predict the domain of fusion protein.
Linker was refereed and modified from previous research study of SRII fusion with HtrII.
false
Leung Wai Tak
annotation2199690
1
RBS 1.0
range2199690
1
42
61
annotation2199694
1
B0015
range2199694
1
2117
2245
annotation2199692
1
SRII-HtrII-Tar
range2199692
1
68
2083
annotation2199693
1
BamHI for C-terminal fusion
range2199693
1
785
790
annotation2199689
1
Constitutive promoter BBa_J23100
range2199689
1
1
41
annotation2199691
1
HindIII for N-terminal fusin
range2199691
1
62
67
BBa_K786002_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagtgaaagaggagaaatactagaagcttatggtgggacttacgaccctcttttggctcggcgcaatcggcatgctcgtcggcacgctcgcgttcgcgtgggccggccgtgacgccggaagcggcgagcgacggtactacgtgacacttgtcggcatcagtggtatcgcagcagtcgcctacgccgttatggcgctgggtgtcggctgggttcccgtggccgaacggactgttttcgtcccccggtacatcgactggattctcacaaccccgctcatcgtctacttcctcgggctgcttgcggggcttgatagtcgggagttcggcatcgtcatcacgctcaacaccgtggtcatgctcgccggcttcgccggggcgatggtgcccggtatcgagcgctacgcgctgttcggcatgggggcggtcgcattcatcggactggtctactacctcgtcgggccgatgaccgaaagcgccagccagcggtcctccggaatcaagtcgctgtacgtccgcctccgaaacctgacggtcgtcctctgggcgatttatccgttcatctggctgcttggaccgccgggcgtggcgctgctgacaccgactgtcgacgtggcgcttatcgtctaccttgacctcgtcacgaaggtcgggttcggcttcatcgcactcgatgctgcggcgacacttcgggccgaacacggcgaatcgctcgctggcgtcgatactgacacgcctgcggtcgccgacggatccgcgtcgaacggcgcgtcggcgatgtcgctgaacgtatcacggctccttctccccggccgtgtccggcacagttatacggggaaaatgggtgccaccttcgcctttgtcggcgcgttaacggtgctcttcggagccatcgcatacggtgaggtaaccgccgccgccgcgaccggcgatgccgcagccgtacaggaggcggcagtatcggccattctcgggctcatcatcctgctcgggatcaacctcgggctcgttgctgccacgctgggcggtgacaccgccgcctcgctttcaacgctggccgcgaaggcctcgcggatgggcgacggcgacctcgatgtcgagcttgagacccgtcgcgaggacgaaatcggcgacctctatgcggccttcgacgagatgcgccagtcggtgcggacaaccgtcactcatgtccgcgaaggttcagatgccatctatgccggtacccgtgaaattgcggcgggcaacaccgatctttcctcccgtactgaacagcaggcatccgcgctggaagaaactgccgccagcatggagcagctcaccgcgacagtgaagcaaaacgccgataacgcccgccaggcctcgcaactggcgcaaagtgcctccgacaccgcccagcacggcggcaaagtggtggatggcgtagtgaaaacgatgcatgagatcgccgatagttcgaagaaaattgccgacattatcagcgttatcgacggtattgccttccagactaatatcctcgcgctgaatgccgcggttgaagccgcgcgtgcgggtgaacagggccgtggttttgccgtggtggcgggtgaagtgcgtaatcttgccagtcgcagcgcccaggcggcaaaagagatcaaagccctcattgaagactccgtctcacgcgttgataccggttcggtgctggtcgaaagcgccggggaaacaatgaacaatatcgtcaatgctgtcactcgcgtgactgacattatgggcgagattgcatcggcatcggatgaacagagccgtggcatcgatcaagtcgcattggcggtttcggaaatggatcgcgtcacgcaacagaacgcatcgctggtgcaggaatcagctgccgccgccgctgcgctggaagaacaggcgagtcgtttaacgcaagcagtttccgcgttccgtctggcagccagcccactcaccaataaaccgcaaacaccatcccgtcctgccagtgagcaaccaccggctcagccacgactgcgaattgctgaacaagatccaaactgggaaacattttgacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z