BBa_K786030
1
BBa_K786030
T7-repeat-spacer-repeat-T7terminator
2012-09-23T11:00:00Z
2015-05-08T01:13:22Z
The 'repeat' sequence in CRISPR/cas system of E.coli strain K12.
It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system.
false
false
_1041_
0
14280
9
Not in stock
false
The repeat-spacer-repeat region design should be consistent with the working principles of E.coli's CRISPR/cas system.
false
ChunHungKong, KenHo, LeonardoKan, Kaiyin Feng
annotation2204276
1
T7 promoter
range2204276
1
1
23
annotation2194793
1
BBa_K606028
range2194793
1
129
264
annotation2194792
1
BBa_I719005
range2194792
1
1
23
annotation2204277
1
repeat-spacer region
range2204277
1
31
120
BBa_K786030_sequence
1
taatacgactcactatagggagatactagagagttccccgcgccagcggggataaaccgcgtgtgtaagtttgcaattccgtttgttgtatgagttccccgcgccagcggggataaaccgtaatactagagtggccggctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z