BBa_K786030 1 BBa_K786030 T7-repeat-spacer-repeat-T7terminator 2012-09-23T11:00:00Z 2015-05-08T01:13:22Z The 'repeat' sequence in CRISPR/cas system of E.coli strain K12. It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system. false false _1041_ 0 14280 9 Not in stock false The repeat-spacer-repeat region design should be consistent with the working principles of E.coli's CRISPR/cas system. false ChunHungKong, KenHo, LeonardoKan, Kaiyin Feng annotation2204276 1 T7 promoter range2204276 1 1 23 annotation2194793 1 BBa_K606028 range2194793 1 129 264 annotation2194792 1 BBa_I719005 range2194792 1 1 23 annotation2204277 1 repeat-spacer region range2204277 1 31 120 BBa_K786030_sequence 1 taatacgactcactatagggagatactagagagttccccgcgccagcggggataaaccgcgtgtgtaagtttgcaattccgtttgttgtatgagttccccgcgccagcggggataaaccgtaatactagagtggccggctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z