BBa_K786031 1 T7RSR T7promoter-RSR-T7teminator 2012-09-23T11:00:00Z 2015-05-08T01:13:22Z The 'repeat' sequence in CRISPR/cas system of E.coli strain K12. It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system. false false _1041_ 0 14280 9 It's complicated true The repeat-spacer-repeat region design should be consistent with the operating principles of E.coli's CRISPR/cas system. false Kaiyin Feng annotation2203441 1 T7-promoter range2203441 1 1 23 annotation2203442 1 repeat-spacer-repeat region range2203442 1 31 803 annotation2203443 1 T7-terminator range2203443 1 812 956 BBa_K786031_sequence 1 taatacgactcactatagggagatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtccgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcgggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgcccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtatactatagggagatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactagagagttccccgcgccagcggggataaaccgtaatactatagggagatactagagagttccccgcgccagcggggataaaccgcgtgtgtaagtttgcaattccgtttgttgtatgagttccccgcgccagcggggataaaccgtaatactagagagttcccgcgccagcggggataaaccgtaatactagagggagatactagagagttccccgcgcagcgggataaaccgtaatactagagtggccggctagtgactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z