LOCUS       BBa_K797013             2232 bp    DNA     linear       28-Nov-2024
DEFINITION  Plasmid Backbone for golden gate assembly
ACCESSION   BBa_K797013
VERSION     BBa_K797013.1  
FEATURES             Location/Qualifiers
     misc_feature    4..9
                     /label=BsaI Recognition site
     misc_feature    11..14
                     /label=BsaI Restriction site
     misc_feature    15..35
                     /label=BioBrick suffix
     primer_bind     157..176
                     /label=VR
     primer_bind     2101..2120
                     /label=VF2
     misc_feature    2199..2219
                     /label=BioBrick prefix
     misc_feature    2219..2222
                     /label=BsaI Restriction site
     misc_feature    2224..2229
                     /label=BsaI Recognition site
ORIGIN
        1 tttggtctca tggatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca
       61 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1021 atatatgagt aaacttggtc tgacagctcg agtcccgtca agtcagcgta atgctctgcc
     1081 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1141 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1201 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1261 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1321 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     1381 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     1441 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     1501 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     1561 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     1621 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     1681 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     1741 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     1801 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     1861 cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt tgaatatggc
     1921 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     1981 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttgttgaa
     2041 taaatcgaac ttttgctgag ttgaaggatc agctcgagtg ccacctgacg tctaagaaac
     2101 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     2161 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagtc
     2221 catgagacca aa
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