BBa_C0061
1
luxI
autoinducer synthetase for AHL
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation1761
1
luxI
range1761
1
1
579
annotation1760
1
LVA
range1760
1
580
611
BBa_K798003
1
BBa_K798003
Ribozyme sequence
2012-09-23T11:00:00Z
2015-05-08T01:13:23Z
Designed and synthetized based on some ribozyme researches
This part is a ribozyme sequence whose design is based on the phenomena that structured RNAs embedded in the untranslated regions (UTRs) of messenger RNAs can regulate gene expression (Martick, Horan et al. 2008). It can be inserted with its target substrate sequence into the 5???UTR region of any genes and as a result down-regulate the expression of the gene or even entirely silence the gene. The ribozyme sequence should be after the substrate sequence. Besides, the length of interval sequence between ribozyme and substrate is alterable from 0 to nearly 1000 bp.
false
false
_1054_
0
14455
9
Not in stock
false
Due to its short length and its down-regulation function for target genes, we insert it into the overlong telomere part as a regulation switch. Once it is damaged with the decrease of telomere, the switch will be off and the expression of the target gene will be normal.
false
Taijie Jin
annotation2195770
1
Ribozyme
range2195770
1
13
41
BBa_K798005
1
BBa_K798005
Recognition sequence of ribozyme sequence
2012-09-23T11:00:00Z
2015-05-08T01:13:23Z
Designed and synthetized based on some ribozyme researches
This part is the recognition sequence of ribozyme sequence. It can be inserted together with the ribozyme sequence into the 5???UTR region of any genes. As a result, it can down-regulate the expression of the gene and even entirely silence the gene. The substrate sequence should be in front of the ribozyme sequence. Besides, the length of interval sequence between ribozyme and substrate is alterable from 0 to nearly 1000 bp.
false
true
_1054_
0
14455
9
It's complicated
false
It should be recognized by the ribozyme sequence so that it can influence the expression of the target gene.
false
Taijie Jin
annotation2195786
1
Substrate of ribozyme
range2195786
1
1
18
BBa_K798006
1
BBa_K798006
substrate sequence-ribozyme sequence
2012-09-23T11:00:00Z
2015-05-08T01:13:23Z
Constructed from the substrate sequence of ribozyme, a 600bp irrelevant DNA sequence and the ribozyme sequence
This part consists of the substrate sequence and the custom Ribozyme Sequence 1. And the length of the interval sequence is approximately 620bp. By adding this part after the 5???UTR region of any genes, the expression can be down-regulated
false
false
_1054_
0
14455
9
Not in stock
false
We link the substrate sequence and ribozyme sequence with a nearly 620bp interval sequence, so after adding this part in the 5??? UTR region of an arbitrary gene, the gene can be knocked down.
false
Taijie Jin
component2248179
1
BBa_K798003
component2248183
1
BBa_C0061
component2248181
1
BBa_K798005
annotation2248183
1
BBa_C0061
range2248183
1
80
697
annotation2248181
1
BBa_K798005
range2248181
1
56
73
annotation2248179
1
BBa_K798003
range2248179
1
1
47
BBa_K798006_sequence
1
gaattctctagaacgcgtctgatgagcgaaacggtatcgatactagttactagagatcgataccgtcacgcgttactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_C0061_sequence
1
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_K798003_sequence
1
gaattctctagaacgcgtctgatgagcgaaacggtatcgatactagt
BBa_K798005_sequence
1
atcgataccgtcacgcgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z