BBa_K798014 1 BBa_K798014 Recognition sequence of ribozyme sequence 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Designed and synthetized based on some ribozyme researches This part is the recognition sequence of ribozyme sequence. It can be inserted together with the ribozyme sequence into the 5???UTR region of any genes. As a result, it can down-regulate the expression of the gene and even entirely silence the gene. The substrate sequence should be in front of the ribozyme sequence. Besides, the length of interval sequence between ribozyme and substrate is alterable from 0 to nearly 1000 bp. false true _1054_ 0 14455 9 It's complicated false It should be recognized by the ribozyme sequence so that it can influence the expression of the target gene. false Taijie Jin annotation2195900 1 ribozyme substrate range2195900 1 1 18 BBa_C0061 1 luxI autoinducer synthetase for AHL 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 Synthesizes 3OC₆HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false false _1_ 0 24 7 In stock false <P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation1761 1 luxI range1761 1 1 579 annotation2213985 1 Help:Barcodes range2213985 1 619 643 annotation7038 1 BBa_C0061 range7038 1 1 618 annotation1760 1 LVA range1760 1 580 611 BBa_K798013 1 BBa_K798013 Ribozyme sequence 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Designed and synthetized based on some ribozyme researches This part is a ribozyme sequence whose design is based on the phenomena that structured RNAs embedded in the untranslated regions (UTRs) of messenger RNAs can regulate gene expression (Martick, Horan et al. 2008). It can be inserted with its target substrate sequence into the 5???UTR region of any genes and as a result down-regulate the expression of the gene or even entirely silence the gene. The ribozyme sequence should be after the substrate sequence. Besides, the length of interval sequence between ribozyme and substrate is alterable from 0 to nearly 1000 bp. false false _1054_ 0 14455 9 It's complicated false Due to its short length and its down-regulation function for target genes, we insert it into the overlong telomere part as a regulation switch. Once it is damaged with the decrease of telomere, the switch will be off and the expression of the target gene will be normal. false Taijie Jin annotation2195897 1 ribozyme range2195897 1 1 40 BBa_K798016 1 BBa_K798016 substrate sequence-ribozyme sequence 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Constructed from the substrate sequence of ribozyme, a 600bp irrelevant DNA sequence and the ribozyme sequence This part consists of the substrate sequence and the custom Ribozyme Sequence 1. And the length of the interval sequence is approximately 620bp. By adding this part after the 5???UTR region of any genes, the expression can be down-regulated. false false _1054_ 0 14455 9 It's complicated false We link the substrate sequence and ribozyme sequence with a nearly 620bp interval sequence, so after adding this part in the 5??? UTR region of an arbitrary gene, the gene can be knocked down. false Taijie Jin component2248217 1 BBa_K798013 component2248213 1 BBa_C0061 component2248211 1 BBa_K798014 annotation2248211 1 BBa_K798014 range2248211 1 1 18 annotation2248213 1 BBa_C0061 range2248213 1 25 642 annotation2248217 1 BBa_K798013 range2248217 1 676 715 BBa_K798013_sequence 1 acgcgtctgatgatgtccgtgaggacgaaacggtatcgat BBa_C0061_sequence 1 atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_K798014_sequence 1 atcgataccgtcacgcgt BBa_K798016_sequence 1 atcgataccgtcacgcgttactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagacgcgtctgatgatgtccgtgaggacgaaacggtatcgat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z