BBa_K798019
1
BBa_K798019
ADH4 homologous arm-TRP1
2012-09-24T11:00:00Z
2015-05-08T01:13:23Z
ADH4 homologous arm was directly synthetized while TRP1 was amplified from the yeast genome by PCR.
This part consists of an ADH4 homologous arm and TRP1 selection marker, so it can be used in the recombination in the left arm of the Chromosome Ⅶ in Saccharomyces cerevisiae.
false
false
_1054_
0
9013
9
Not in stock
false
In order to improve the success rate of recombination, the homologous arm is 60bp.
false
Chao Zhang
component2197605
1
BBa_K798017
component2197608
1
BBa_K798018
annotation2197605
1
BBa_K798017
range2197605
1
1
60
annotation2197608
1
BBa_K798018
range2197608
1
69
1265
BBa_K798017
1
BBa_K798017
ADH4 homologous arm
2012-09-24T11:00:00Z
2015-05-08T01:13:23Z
synthetized directly according to the genome
This part is a 60bp homologous arm which is generally used as the recombination site in the left arm of Chromosome Ⅶ in Saccharomyces cerevisiae.
false
false
_1054_
0
9013
9
Not in stock
false
Considering the success rate of recombination, the length of the homologous arm is 60bp.
false
Chao Zhang
annotation2197520
1
ADH4 homologous arm
range2197520
1
1
60
BBa_K798018
1
BBa_K798018
TRP1
2012-09-24T11:00:00Z
2015-05-08T01:13:23Z
Amplified from the Saccharomyces cerevisiae genome by PCR
TRP1 encodes phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis, so it's always used as the selection marker during the transformation or recombination in the yeast.
false
false
_1054_
0
9013
9
Not in stock
false
The part has included the TRP1 promoter.
false
Chao Zhang
annotation2197585
1
TRP1 promoter
range2197585
1
1
1197
annotation2197597
1
TRP1
range2197597
1
473
1147
BBa_K798019_sequence
1
taaagcaccttcaccaaagaaagagattggtggaatgtaaaacccagtaacggaagacattactagagtcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatagatcaacgacattactatatatataatataggaagcatttaatagaacagcatcgtaatatatgtgtactttgcagttatgacgccagatggcagtagtggaagatattctttattgaaaaatagcttgtcaccttacgtacaatcttgatccggagcttttctttttttgccgattaagaattaattcggtcgaaaaaagaaaaggagagggccaagagggagggcattggtgactattgagcacgtgagtatacgtgattaagcacacaaaggcagcttggagtatgtctgttattaatttcacaggtagttctggtccattggtgaaagtttgcggcttgcagagcacagaggccgcagaatgtgctctagattccgatgctgacttgctgggtattatatgtgtgcccaatagaaagagaacaattgacccggttattgcaaggaaaatttcaagtcttgtaaaagcatataaaaatagttcaggcactccgaaatacttggttggcgtgtttcgtaatcaacctaaggaggatgttttggctctggtcaatgattacggcattgatatcgtccaactgcatggagatgagtcgtggcaagaataccaagagttcctcggtttgccagttattaaaagactcgtatttccaaaagactgcaacatactactcagtgcagcttcacagaaacctcattcgtttattcccttgtttgattcagaagcaggtgggacaggtgaacttttggattggaactcgatttctgactgggttggaaggcaagagagccccgaaagcttacattttatgttagctggtggactgacgccagaaaatgttggtgatgcgcttagattaaatggcgttattggtgttgatgtaagcggaggtgtggagacaaatggtgtaaaagactctaacaaaatagcaaatttcgtcaaaaatgctaagaaataggttattactgagtagtatttatttaagtattgtttgtgcacttgccgatc
BBa_K798017_sequence
1
taaagcaccttcaccaaagaaagagattggtggaatgtaaaacccagtaacggaagacat
BBa_K798018_sequence
1
tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatagatcaacgacattactatatatataatataggaagcatttaatagaacagcatcgtaatatatgtgtactttgcagttatgacgccagatggcagtagtggaagatattctttattgaaaaatagcttgtcaccttacgtacaatcttgatccggagcttttctttttttgccgattaagaattaattcggtcgaaaaaagaaaaggagagggccaagagggagggcattggtgactattgagcacgtgagtatacgtgattaagcacacaaaggcagcttggagtatgtctgttattaatttcacaggtagttctggtccattggtgaaagtttgcggcttgcagagcacagaggccgcagaatgtgctctagattccgatgctgacttgctgggtattatatgtgtgcccaatagaaagagaacaattgacccggttattgcaaggaaaatttcaagtcttgtaaaagcatataaaaatagttcaggcactccgaaatacttggttggcgtgtttcgtaatcaacctaaggaggatgttttggctctggtcaatgattacggcattgatatcgtccaactgcatggagatgagtcgtggcaagaataccaagagttcctcggtttgccagttattaaaagactcgtatttccaaaagactgcaacatactactcagtgcagcttcacagaaacctcattcgtttattcccttgtttgattcagaagcaggtgggacaggtgaacttttggattggaactcgatttctgactgggttggaaggcaagagagccccgaaagcttacattttatgttagctggtggactgacgccagaaaatgttggtgatgcgcttagattaaatggcgttattggtgttgatgtaagcggaggtgtggagacaaatggtgtaaaagactctaacaaaatagcaaatttcgtcaaaaatgctaagaaataggttattactgagtagtatttatttaagtattgtttgtgcacttgccgatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z