LOCUS BBa_K801000 4923 bp DNA linear 13-Jun-2024 DEFINITION pTUM100 yeast shuttle vector based on pYES2 ACCESSION BBa_K801000 VERSION BBa_K801000.1 FEATURES Location/Qualifiers primer_bind 4..23 /label=T7-Promoter misc_feature 36..134 /label=RFC25 MCS misc_feature 78..101 /label=Strep-tag II misc_feature 148..396 /label=CYC1-Terminator misc_feature 578..1251 /label=pUC-origin CDS 1396..2256 /label=AmpR CDS 2274..3381 /label=URA3 misc_feature 3385..4856 /label=2 micron (??) origin ORIGIN 1 ctgtaatacg actcactata gggaatatta agcttgaatt cgcggccgct tctagaggta 61 ccctcgaggc cggcgcttgg tctcacccgc agttcgaaaa ataaaccggt taatactagt 121 agcggccgct gcagactaga gggccgcatc atgtaattag ttatgtcacg cttacattca 181 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 241 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 301 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 361 gagaaggttt tgggacgctc gaaggcttta atttgcggcc ctgcattaat gaatcggcca 421 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 481 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 601 gcccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 1021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 1081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 1141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 1261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1381 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 1441 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggagcg 1501 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 1561 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 1621 atccgcctcc attcagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 1681 taatagtttg cgcaacgttg ttggcattgc tacaggcatc gtggtgtcac tctcgtcgtt 1741 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 1801 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 1861 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 1921 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 1981 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aatagtgtat cacatagcag 2041 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 2101 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 2161 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 2221 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatgggtaat 2281 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat 2341 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt 2401 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca 2461 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat 2521 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca 2581 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc 2641 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat 2701 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa 2761 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct 2821 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt 2881 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg 2941 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga 3001 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac 3061 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga 3121 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctacaggtt 3181 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat 3241 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag 3301 attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat 3361 gatgaattga attgaaaagc tagcttatcg atgataagct gtcaaagatg agaattaatt 3421 ccacggacta tagactatac tagatactcc gtctactgta cgatacactt ccgctcaggt 3481 ccttgtcctt taacgaggcc ttaccactct tttgttactc tattgatcca gctcagcaaa 3541 ggcagtgtga tctaagattc tatcttcgcg atgtagtaaa actagctaga ccgagaaaga 3601 gactagaaat gcaaaaggca cttctacaat ggctgccatc attattatcc gatgtgacgc 3661 tacagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat atccgacaaa 3721 ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa catccgaacc 3781 tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat atagtctagc 3841 gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca tctattgcat 3901 aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt gcacttcaat 3961 agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga 4021 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg 4081 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa 4141 cgcgacgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 4201 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa 4261 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct 4321 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta 4381 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc 4441 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga 4501 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat 4561 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg 4621 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta 4681 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt 4741 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga 4801 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa 4861 gctccacccc ggttgataat cagaaaagcc ccaaaaacag gaagattgta taagcaaata 4921 ttt //