LOCUS BBa_K801001 5315 bp DNA linear 22-Dec-2024 DEFINITION pTUM101 yeast shuttle vector with pTEF1 promoter ACCESSION BBa_K801001 VERSION BBa_K801001.1 FEATURES Location/Qualifiers primer_bind 4..23 /label=T7-Promoter promoter 58..505 /label=TEF1-Promoter misc_feature 540..787 /label=CYC1-Terminator misc_feature 970..1643 /label=pUC-origin CDS 1788..2647 /label=AmpR CDS 2666..3773 /label=URA3 misc_feature 3777..5248 /label=2 micron (??) origin ORIGIN 1 ctgtaatacg actcactata gggaatatta agcttgaatt cgcggccgct tctagagtca 61 cacccaatcc cccacaagtg atcccccaca caccatagct tcaaaatgtt tctactcctt 121 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa 181 gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt acccgtacta 241 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat 301 aaaaattttt atcacgtttc tttttcttga aaattttttt ttttgatttt tttctctttc 361 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt 421 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc 481 aatctaatct aagttttaat tacaatacta gtagcggccg ctgcagacta gagggccgca 541 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg 601 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt 661 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt 721 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt 781 taatttgcgg ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 841 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 961 ggaaagaaca tgtgagcaaa aggccagcaa aagcccagga accgtaaaaa ggccgcgttg 1021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 1081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 1141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 1201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 1261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 1321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 1381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 1441 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 1501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 1621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 1681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 1741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 1801 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 1861 cccgtcgtgt agataactac gatacgggag cgcttaccat ctggccccag tgctgcaatg 1921 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 1981 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccattcagtc tattaattgt 2041 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttggcatt 2101 gctacaggca tcgtggtgtc actctcgtcg tttggtatgg cttcattcag ctccggttcc 2161 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2221 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 2281 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 2341 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 2401 tcaatacggg ataatagtgt atcacatagc agaactttaa aagtgctcat cattggaaaa 2461 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 2521 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 2581 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 2641 atactcatac tcttcctttt tcaatgggta ataactgata taattaaatt gaagctctaa 2701 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc 2761 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat 2821 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca 2881 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac 2941 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa 3001 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt 3061 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt 3121 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac 3181 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca 3241 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact 3301 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat 3361 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt 3421 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat 3481 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt 3541 tcgacatgat ttatcttcgt ttcctacagg tttttgttct gtgcagttgg gttaagaata 3601 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt 3661 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaagaa 3721 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa gctagcttat 3781 cgatgataag ctgtcaaaga tgagaattaa ttccacggac tatagactat actagatact 3841 ccgtctactg tacgatacac ttccgctcag gtccttgtcc tttaacgagg ccttaccact 3901 cttttgttac tctattgatc cagctcagca aaggcagtgt gatctaagat tctatcttcg 3961 cgatgtagta aaactagcta gaccgagaaa gagactagaa atgcaaaagg cacttctaca 4021 atggctgcca tcattattat ccgatgtgac gctacagctt ctcaatgata ttcgaatacg 4081 ctttgaggag atacagccta atatccgaca aactgtttta cagatttacg atcgtacttg 4141 ttacccatca ttgaattttg aacatccgaa cctgggagtt ttccctgaaa cagatagtat 4201 atttgaacct gtataataat atatagtcta gcgctttacg gaagacaatg tatgtatttc 4261 ggttcctgga gaaactattg catctattgc ataggtaatc ttgcacgtcg catccccggt 4321 tcattttctg cgtttccatc ttgcacttca atagcatatc tttgttaacg aagcatctgt 4381 gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct 4441 gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat 4501 ctgtgcttca tttttgtaaa acaaaaatgc aacgcgacga gagcgctaat ttttcaaaca 4561 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa 4621 caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct 4681 aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga 4741 taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc 4801 tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg 4861 ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc 4921 atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa 4981 cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg 5041 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta 5101 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg 5161 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc 5221 aatgtttgtg gaagcggtat tcgcaatggg aagctccacc ccggttgata atcagaaaag 5281 ccccaaaaac aggaagattg tataagcaaa tattt //