LOCUS BBa_K801002 5514 bp DNA linear 22-Nov-2024 DEFINITION pTUM102 yeast shuttle vector with pTEF2 promoter ACCESSION BBa_K801002 VERSION BBa_K801002.1 FEATURES Location/Qualifiers promoter 58..705 /label=TEF2-Promoter misc_feature 739..984 /label=CYC1-T misc_feature 1169..1842 /label=pUC-origin CDS 1987..2846 /label=AmpR CDS 2865..3972 /label=URA3 misc_feature 3976..5447 /label=2 micron (??) origin primer_bind 4..23 /label=T7-Promoter ORIGIN 1 ctgtaatacg actcactata gggaatatta agcttgaatt cgcggccgct tctagaggct 61 acctatattc caccataaca tcaatcatgc ggttgctggt gtatttacca ataatgttta 121 atgtatatat atatatatat atatggggcc gtatacttac atatagtaga tgtcaagcgt 181 aggcgcttcc cctgccggct gtgagggcgc cataaccaag gtatctatag accgccaatc 241 agcaaactac ctccgtacat tcatgttgca cccacacatt tatacaccca gaccgcgaca 301 aattacccat aaggttgttt gtgacggcgt cgtacaagag aacgtgggaa ctttttaggc 361 tcaccaaaaa agaaagaaaa aatacgagtt gctgacagaa gcctcaagaa aaaaaaaatt 421 cttcttcgac tatgctggag gcagagatga tcgagccggt agttaactat atatagctaa 481 attggttcca tcaccttctt ttctggtgtc gctccttcta gtgctatttc tggcttttcc 541 tatttttttt tttccatttt tctttctctc tttctaatat ataaattctc ttgcattttc 601 tatttttctc tctatctatt ctacttgttt attcccttca aggttttttt ttaaggagta 661 cttgttttta gaatatacgg tcaacgaact ataattaact aaactactag tagcggccgc 721 tgcagactag agggccgcat catgtaatta gttatgtcac gcttacattc acgccctccc 781 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt 841 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt 901 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt 961 ttgggacgct cgaaggcttt aatttgcggc cctgcattaa tgaatcggcc aacgcgcggg 1021 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 1081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 1141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa agcccaggaa 1201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 1261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 1321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 1381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 1441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 1501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 1561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 1621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 1681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 1741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 1801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 1861 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 1921 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1981 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 2041 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc gcttaccatc 2101 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 2161 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 2221 cattcagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 2281 gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca ctctcgtcgt ttggtatggc 2341 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 2401 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 2461 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 2521 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 2581 gagttgctct tgcccggcgt caatacggga taatagtgta tcacatagca gaactttaaa 2641 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 2701 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 2761 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 2821 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgggtaa taactgatat 2881 aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt 2941 ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt 3001 tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg 3061 tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc 3121 ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca 3181 cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca 3241 acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac 3301 atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca 3361 atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat 3421 acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg 3481 aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg 3541 gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct 3601 tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt 3661 tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca 3721 cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctacaggt ttttgttctg 3781 tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata 3841 tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa 3901 tcaaaaaaat ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg 3961 aattgaaaag ctagcttatc gatgataagc tgtcaaagat gagaattaat tccacggact 4021 atagactata ctagatactc cgtctactgt acgatacact tccgctcagg tccttgtcct 4081 ttaacgaggc cttaccactc ttttgttact ctattgatcc agctcagcaa aggcagtgtg 4141 atctaagatt ctatcttcgc gatgtagtaa aactagctag accgagaaag agactagaaa 4201 tgcaaaaggc acttctacaa tggctgccat cattattatc cgatgtgacg ctacagcttc 4261 tcaatgatat tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac 4321 agatttacga tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt 4381 tccctgaaac agatagtata tttgaacctg tataataata tatagtctag cgctttacgg 4441 aagacaatgt atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct 4501 tgcacgtcgc atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct 4561 ttgttaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa 4621 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc 4681 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgacgag 4741 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 4801 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 4861 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 4921 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 4981 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 5041 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 5101 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 5161 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 5221 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 5281 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 5341 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 5401 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatggga agctccaccc 5461 cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat attt //