LOCUS BBa_K801003 5585 bp DNA linear 22-Nov-2024 DEFINITION pTUM103 yeast shuttle vector with pADH1 promoter ACCESSION BBa_K801003 VERSION BBa_K801003.1 FEATURES Location/Qualifiers misc_feature 810..1057 /label=CYC1-Terminator CDS 2936..4043 /label=URA3 CDS 2058..2917 /label=AmpR promoter 58..775 /label=ADH1-Promoter primer_bind 4..23 /label=T7-Promoter misc_feature 4047..5518 /label=2 micron (??) origin misc_feature 1240..1913 /label=pUC-origin ORIGIN 1 ctgtaatacg actcactata gggaatatta agcttgaatt cgcggccgct tctagagata 61 tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa ccaaacccat 121 acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg cggaggggag 181 atatacaata gaacagatac cagacaagac ataatgggct aaacaagact acaccaatta 241 cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga cttgatagcc 301 atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa gtaataatag 361 gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt gtctcaccat 421 atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag 481 caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac acgaaacttt 541 ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc 601 agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc 661 tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct 721 ttttctgcac aatatttcaa gctataccaa gcatacaatc aactatctca tatactacta 781 gtagcggccg ctgcagacta gagggccgca tcatgtaatt agttatgtca cgcttacatt 841 cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct 901 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa 961 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc 1021 ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccctgcatta atgaatcggc 1081 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 1141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 1201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1261 aagcccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 1321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 1741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 2041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 2101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 2161 cgcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 2221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 2281 ttatccgcct ccattcagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 2341 gttaatagtt tgcgcaacgt tgttggcatt gctacaggca tcgtggtgtc actctcgtcg 2401 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 2461 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 2521 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 2581 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 2641 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataatagtgt atcacatagc 2701 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2761 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2821 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2881 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatgggta 2941 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt 3001 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt 3061 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa 3121 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca 3181 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt 3241 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct 3301 tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca 3361 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca 3421 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg 3481 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt 3541 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg 3601 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg 3661 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac 3721 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat 3781 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctacagg 3841 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac 3901 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg 3961 agattaccga atcaaaaaaa tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa 4021 atgatgaatt gaattgaaaa gctagcttat cgatgataag ctgtcaaaga tgagaattaa 4081 ttccacggac tatagactat actagatact ccgtctactg tacgatacac ttccgctcag 4141 gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc cagctcagca 4201 aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta gaccgagaaa 4261 gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac 4321 gctacagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca 4381 aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa 4441 cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta 4501 gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc 4561 ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca 4621 atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 4681 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 4741 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 4801 aacgcgacga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag 4861 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac 4921 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc 4981 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt 5041 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc 5101 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc 5161 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg 5221 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta 5281 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac 5341 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag 5401 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca 5461 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatggg 5521 aagctccacc ccggttgata atcagaaaag ccccaaaaac aggaagattg tataagcaaa 5581 tattt //