LOCUS BBa_K801004 5837 bp DNA linear 24-Nov-2024 DEFINITION pTUM104 yeast shuttle vector with GAL1 promoter ACCESSION BBa_K801004 VERSION BBa_K801004.1 FEATURES Location/Qualifiers misc_feature 44..292 /label=CYC1 transcription terminator misc_feature 474..1147 /label=pUC1 origin misc_feature 1292..2152 /label=Ampicillin misc_feature 2170..3277 /label=URA3 misc_feature 3281..4752 /label=2 ?? origin misc_feature 4820..5275 /label=f1 origin promoter 5293..5743 /label=PGAL1 misc_feature 5767..5786 /label=T7 promoter/priming site ORIGIN 1 accggttaat actagtagcg gccgctgcag actagagggc cgcatcatgt aattagttat 61 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag 121 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt 181 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat 241 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcggccctgc 301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 481 caaaaggcca gcaaaagccc aggaaccgta aaaaggccgc gttgctggcg tttttccata 541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 961 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 1021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 1081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 1141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 1201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 1261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 1321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 1381 ctacgatacg ggagcgctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 1441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 1501 gtggtcctgc aactttatcc gcctccattc agtctattaa ttgttgccgg gaagctagag 1561 taagtagttc gccagttaat agtttgcgca acgttgttgg cattgctaca ggcatcgtgg 1621 tgtcactctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 1681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 1741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 1801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 1861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 1921 gtgtatcaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 1981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 2041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 2101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 2161 tttttcaatg ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat 2221 acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc 2281 ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa 2341 tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct 2401 atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa 2461 ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa 2521 atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga 2581 gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc 2641 tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat 2701 gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc 2761 aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt 2821 tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag 2881 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac 2941 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc 3001 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct 3061 tcgtttccta caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt 3121 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc 3181 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa 3241 agaataaaaa aaaaatgatg aattgaattg aaaagctagc ttatcgatga taagctgtca 3301 aagatgagaa ttaattccac ggactataga ctatactaga tactccgtct actgtacgat 3361 acacttccgc tcaggtcctt gtcctttaac gaggccttac cactcttttg ttactctatt 3421 gatccagctc agcaaaggca gtgtgatcta agattctatc ttcgcgatgt agtaaaacta 3481 gctagaccga gaaagagact agaaatgcaa aaggcacttc tacaatggct gccatcatta 3541 ttatccgatg tgacgctaca gcttctcaat gatattcgaa tacgctttga ggagatacag 3601 cctaatatcc gacaaactgt tttacagatt tacgatcgta cttgttaccc atcattgaat 3661 tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga acctgtataa 3721 taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc tggagaaact 3781 attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt tctgcgtttc 3841 catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa 3901 caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca 3961 gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg 4021 taaaacaaaa atgcaacgcg acgagagcgc taatttttca aacaaagaat ctgagctgca 4081 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt 4141 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga 4201 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt 4261 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag 4321 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag 4381 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga 4441 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt 4501 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta 4561 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa 4621 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat 4681 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg 4741 gtattcgcaa tgggaagctc caccccggtt gataatcaga aaagccccaa aaacaggaag 4801 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 4861 ttttgttaaa tcagctcatt ttttaacgaa tagcccgaaa tcggcaaaat cccttataaa 4921 tcaaaagaat agaccgagat agggttgagt gttgttccag tttccaacaa gagtccacta 4981 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaggg tctatcaggg cgatggccca 5041 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcagtaaat 5101 cggaagggta aacggatgcc cccatttaga gcttgacggg gaaagcaggc gaacgtggcg 5161 agaaaggaag ggaagaaagc gaaaggagcg ggggctaggg cggtgggaag tgtaggggtc 5221 acgctgggcg taaccaccac acccgccgcg cttaatgggg cgctacaggg cgcgtgggga 5281 tgatccacga gtacggatta gaagccgccg agcgggtgac agccctccga aggaagactc 5341 tcctccgtgc gtcctcgtct tcgccggtcg cgttcctgaa acgcagatgt gcctcgcgcc 5401 gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa 5461 attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa caaccatagg 5521 atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga 5581 tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt aactaatact 5641 ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat caacaaaaaa 5701 ttgttaatat acctctatac tttaacgtca aggagaaaaa accccggatc ggactactag 5761 cagctgtaat acgactcact atagggaata ttaagcttga attcgcggcc gcttctagag 5821 gtaccctcga ggccggc //