LOCUS       BBa_K801039              804 bp    DNA     linear       23-May-2024
DEFINITION  SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based 
            light-switchable promoter system)
ACCESSION   BBa_K801039
VERSION     BBa_K801039.1  
FEATURES             Location/Qualifiers
     misc_feature    31..51
                     /label=SV40 nuclear localization sequence (recognized by 
                     importin alpha)
     CDS             70..408
                     /label=GAL4 activation domain (GAL4AD)
     CDS             503..801
                     /label=PIF3 (First 100 N-terminal amino acids)
     misc_feature    499..504
                     /label=EcoRI/MfeI fusion scar
     misc_feature    409..498
                     /label=Linker
     promoter        414..436
                     /label=T7 promoter (for sequencing)
     misc_feature    457..477
                     /label=HA epitope tag
ORIGIN
        1 atggataaag cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt
       61 accgccgcca attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc
      121 actaacagta gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca
      181 caaccaattg cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt
      241 aaaattgatg atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg
      301 tataacgcgt ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat
      361 aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg
      421 actcactata gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat
      481 atggccatgg aggccagtga attgcctctg tttgaacttt tcaggctcac caaagctaag
      541 cttgaatctg ctcaagacag gaacccttct ccacctgtag atgaagttgt ggagctggtg
      601 tgggaaaatg gtcagatatc aactcaaagt cagtcaagta gatcgaggaa cattcctcca
      661 ccacaagcaa actcttcaag agctagagag attggaaatg gctcaaagac gactatggtg
      721 gacgagatcc ctatgtcagt gccatcacta atgacgggtt tgagtcaaga cgatgacttt
      781 gttccatggt tgaatcatca ttaa
//