LOCUS BBa_K801042 6063 bp DNA linear 22-Nov-2024 DEFINITION GAL4 based yeast light-switchable promoter system ACCESSION BBa_K801042 VERSION BBa_K801042.1 FEATURES Location/Qualifiers promoter 1..450 /label=BBa_K319003 (pTEF1, TEF1 promoter) CDS 456..1259 /label=BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3) misc_feature 465..521 /label=SV40 NLS (SV40 nuclear localization signal) CDS 525..863 /label=GAL4AD (GAL4 transcriptonal activation domain) misc_feature 864..959 /label=Linker promoter 869..891 /label=T7 promoter (for sequencing) misc_feature 912..932 /label=HA epitope tage CDS 960..1256 /label=PIF3 (First 100 N-terminal amino acids) misc_feature 1268..1774 /label=BBa_K801011, (tTEF1, TEF1 terminator) promoter 1782..2231 /label=BBa_K319003 (pTEF1, TEF1 promoter) CDS 2237..5548 /label=SV40NLS-PhyB-Linker-GAL4DBD misc_feature 2246..2302 /label=SV40 NLS (SV40 nuclear localization signal) misc_feature 2246..5539 /label=BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD) CDS 2309..5029 /label=Phytochrome B (PhyB, 908 first N-terminal amino acids) misc_feature 5036..5095 /label=20 amino acids long linker (BBa_K365005) CDS 5102..5539 /label=GAL4DBD (GAL4 DNA binding domain) misc_feature 5557..6063 /label=BBa_K801011, (tTEF1, TEF1 terminator) ORIGIN 1 atcacaccca atcccccaca agtgatcccc cacacaccat agcttcaaaa tgtttctact 61 ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac 121 ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt 181 actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg 241 caataaaaat ttttatcacg tttctttttc ttgaaaattt ttttttttga tttttttctc 301 tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc tcaagtttca 361 gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag aaagaaagca 421 tagcaatcta atctaagttt taattacaaa actagatgga taaagcggaa ttaattcccg 481 agcctccaaa aaagaagaga aaggtcgaat tgggtaccgc cgccaatttt aatcaaagtg 541 ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc 601 tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc 661 atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa 721 aaccactgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag 781 ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat gatgaagata 841 ccccaccaaa cccaaaaaaa gagatcttta atacgactca ctatagggcg agcgccgcca 901 tggagtaccc atacgacgta ccagattacg ctcatatggc catggaggcc agtgaattgc 961 ctctgtttga acttttcagg ctcaccaaag ctaagcttga atctgctcaa gacaggaacc 1021 cttctccacc tgtagatgaa gttgtggagc tggtgtggga aaatggtcag atatcaactc 1081 aaagtcagtc aagtagatcg aggaacattc ctccaccaca agcaaactct tcaagagcta 1141 gagagattgg aaatggctca aagacgacta tggtggacga gatccctatg tcagtgccat 1201 cactaatgac gggtttgagt caagacgatg actttgttcc atggttgaat catcattaat 1261 actagaggga gattgataag acttttctag ttgcatatct tttatattta aatcttatct 1321 attagttaat tttttgtaat ttatccttat atatagtctg gttattctaa aatatcattt 1381 cagtatctaa aaattcccct cttttttcag ttatatctta acaggcgaca gtccaaatgt 1441 tgatttatcc cagtccgatt catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac 1501 atcagtaata gtgcctctta cttgcctcat agaatttctt tctcttaacg tcaccgtttg 1561 tcttttatag tttcgaaatc tatggtgata ccaaatggtg ttcccaattc atcgttacgg 1621 gcgtattttt taccaattga agtattggaa tcgtcaattt taaagtatat ctctctttta 1681 cgtaaagcct gcgagatcct cttaagtata gcggggaagc catcgttatt cgatattgtc 1741 gtaacaaata ctttgagcgg cgctatctgt aatgtactag aatcacaccc aatcccccac 1801 aagtgatccc ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga 1861 ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa 1921 tttcccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga 1981 aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac 2041 gtttcttttt cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg 2101 atatttaagt taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct 2161 attacaactt tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt 2221 ttaattacaa aactagatgg ccggcgcgga attaattccc gagcctccaa aaaagaagag 2281 aaaggtcgaa ttgggtaccg ccaccggcgt ttccggagtc gggggtagtg gcggtggccg 2341 tggcggtggc cgtggcggag aagaagaacc gtcgtcaagt cacactccta ataaccgaag 2401 aggaggagaa caagctcaat cgtcgggaac gaaatctctc agaccaagaa gcaacactga 2461 atcaatgagc aaagcaattc aacagtacac cgtcgacgca agactccacg ccgttttcga 2521 acaatccggc gaatcaggga aatcattcga ctactcacaa tcactcaaaa cgacgacgta 2581 cggttcctct gtacctgagc aacagatcac agcttatctc tctcgaatcc agcgaggtgg 2641 ttacattcag cctttcggat gtatgatcgc cgtcgatgaa tccagtttcc ggatcatcgg 2701 ttacagtgaa aacgccagag aaatgttagg gattatgcct caatctgttc ctactcttga 2761 gaaacctgag attctagcta tgggaactga tgtgagatct ttgttcactt cttcgagctc 2821 gattctactc gagcgtgctt tcgttgctcg agagattacc ttgttaaatc cggtttggat 2881 ccattccaag aatactggta aaccgtttta cgccattctt cataggattg atgttggtgt 2941 tgttattgat ttagagccag ctagaactga agatcctgcg ctttctattg ctggtgctgt 3001 tcaatcgcag aaactcgcgg ttcgtgcgat ttctcagtta caggctcttc ctggtggaga 3061 tattaagctt ttgtgtgaca ctgtcgtgga aagtgtgagg gacttgactg gttatgatcg 3121 tgttatggtt tataagtttc atgaagatga gcatggagaa gttgtagctg agagtaaacg 3181 agacgattta gagccttata ttggactgca ttatcctgct actgatattc ctcaagcgtc 3241 aaggttcttg tttaagcaga accgtgtccg aatgatagta gattgcaatg ccacacctgt 3301 tcttgtggtc caggacgata ggctaactca gtctatgtgc ttggttggtt ctactcttag 3361 ggctcctcat ggttgtcact ctcagtatat ggctaacatg ggatctattg cgtctttagc 3421 aatggcggtt ataatcaatg gaaatgaaga tgatgggagc aatgtagcta gtggaagaag 3481 ctcgatgagg ctttggggtt tggttgtttg ccatcacact tcttctcgct gcataccgtt 3541 tccgctaagg tatgcttgtg agtttttgat gcaggctttc ggtttacagt taaacatgga 3601 attgcagtta gctttgcaaa tgtcagagaa acgcgttttg agaacgcaga cactgttatg 3661 tgatatgctt ctgcgtgact cgcctgctgg aattgttaca cagagtccca gtatcatgga 3721 cttagtgaaa tgtgacggtg cagcatttct ttaccacggg aagtattacc cgttgggtgt 3781 tgctcctagt gaagttcaga taaaagatgt tgtggagtgg ttgcttgcga atcatgcgga 3841 ttcaaccgga ttaagcactg atagtttagg cgatgcgggg tatcccggtg cagctgcgtt 3901 aggggatgct gtgtgcggta tggcagttgc atatatcaca aaaagagact ttcttttttg 3961 gtttcgatct cacactgcga aagaaatcaa atggggaggc gctaagcatc atccggagga 4021 taaagatgat gggcaacgaa tgcatcctcg ttcgtccttt caggcttttc ttgaagttgt 4081 taagagccgg agtcagccat gggaaactgc ggaaatggat gcgattcact cgctccagct 4141 tattctgaga gactctttta aagaatctga ggcggctatg aactctaaag ttgtggatgg 4201 tgtggttcag ccatgtaggg atatggcggg ggaacagggg attgatgagt taggtgcagt 4261 tgcaagagag atggttaggc tcattgagac tgcaactgtt cctatattcg ctgtggatgc 4321 cggaggctgc atcaatggat ggaacgctaa gattgcagag ttgacaggtc tctcagttga 4381 agaagctatg gggaagtctc tggtttctga tttaatatac aaagagaatg aagcaactgt 4441 caataagctt ctttctcgtg ctttgagagg ggacgaggaa aagaatgtgg aggttaagct 4501 gaaaactttc agccccgaac tacaagggaa agcagttttt gtggttgtga atgcttgttc 4561 cagcaaggac tacttgaaca acattgtcgg cgtttgtttt gttggacaag acgttacgag 4621 tcagaaaatc gtaatggata agttcatcaa catacaagga gattacaagg ctattgtaca 4681 tagcccaaac cctctaatcc cgccaatttt tgctgctgac gagaacacgt gctgcctgga 4741 atggaacatg gcgatggaaa agcttacggg ttggtctcgc agtgaagtga ttgggaaaat 4801 gattgtcggg gaagtgtttg ggagctgttg catgctaaag ggtcctgatg ctttaaccaa 4861 gttcatgatt gtattgcata atgcgattgg tggccaagat acggataagt tccctttccc 4921 attctttgac cgcaatggga agtttgttca ggctctattg actgcaaaca agcgggttag 4981 cctcgaggga aaggttattg gggctttctg tttcttgcaa atcccgagca ccggcgcgag 5041 cggcgcgggc ggcagcgaag gcggcggcag cgaaggcggc accagcggcg cgaccaccgg 5101 caagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg 5161 ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc 5221 tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct 5281 agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt 5341 gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa 5401 tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac 5461 attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca 5521 aagacagttg actgtatcga ccggttaata ctagagggag attgataaga cttttctagt 5581 tgcatatctt ttatatttaa atcttatcta ttagttaatt ttttgtaatt tatccttata 5641 tatagtctgg ttattctaaa atatcatttc agtatctaaa aattcccctc ttttttcagt 5701 tatatcttaa caggcgacag tccaaatgtt gatttatccc agtccgattc atcagggttg 5761 tgaagcattt tgtcaatggt cgaaatcaca tcagtaatag tgcctcttac ttgcctcata 5821 gaatttcttt ctcttaacgt caccgtttgt cttttatagt ttcgaaatct atggtgatac 5881 caaatggtgt tcccaattca tcgttacggg cgtatttttt accaattgaa gtattggaat 5941 cgtcaatttt aaagtatatc tctcttttac gtaaagcctg cgagatcctc ttaagtatag 6001 cggggaagcc atcgttattc gatattgtcg taacaaatac tttgagcggc gctatctgta 6061 atg //