##gff-version 3
##sequence-region BBa_K801043 1 6228
BBa_K801043	.	engineered_region	2246	5704	.	+	0	ID=annotation2198814;Name=BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
BBa_K801043	.	CDS	2237	5713	.	+	0	ID=annotation2198807;Name=SV40NLS-PhyB-Linker-LexA
BBa_K801043	.	tag	465	521	.	+	0	ID=annotation2198799;Name=SV40 NLS (SV40 nuclear localization signal)
BBa_K801043	.	sequence_feature	1268	1774	.	+	0	ID=annotation2198805;Name=BBa_K801011, (tTEF1, TEF1 terminator)
BBa_K801043	.	promoter	869	891	.	+	0	ID=annotation2198802;Name=T7 promoter (for sequencing)
BBa_K801043	.	tag	5036	5095	.	+	0	ID=annotation2198810;Name=BBa_K365005 (20 amino acids long linker)
BBa_K801043	.	CDS	456	1259	.	+	0	ID=annotation2198798;Name=BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
BBa_K801043	.	tag	912	932	.	+	0	ID=annotation2198803;Name=HA epitope tage
BBa_K801043	.	CDS	525	863	.	+	0	ID=annotation2198800;Name=GAL4AD (GAL4 transcriptonal activation domain)
BBa_K801043	.	CDS	960	1256	.	+	0	ID=annotation2198804;Name=PIF3 (First 100 N-terminal amino acids)
BBa_K801043	.	sequence_feature	5722	6228	.	+	0	ID=annotation2198812;Name=BBa_K801011, (tTEF1, TEF1 terminator)
BBa_K801043	.	tag	864	959	.	+	0	ID=annotation2198801;Name=Linker
BBa_K801043	.	promoter	1	450	.	+	0	ID=annotation2198797;Name=	BBa_K319003 (pTEF1, TEF1 promoter) 
BBa_K801043	.	tag	2246	2302	.	+	0	ID=annotation2198808;Name=SV40 NLS (SV40 nuclear localization signal)
BBa_K801043	.	CDS	2309	5029	.	+	0	ID=annotation2198809;Name=Phytochrome B (PhyB, 908 first N-terminal amino acids)
BBa_K801043	.	promoter	1782	2231	.	+	0	ID=annotation2198806;Name=BBa_K319003 (pTEF1, TEF1 promoter)
BBa_K801043	.	CDS	5102	5704	.	+	0	ID=annotation2198811;Name=LexA
>BBa_K801043
atcacacccaatcccccacaagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttccaga
ttttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctc
tagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaa
aggcaataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttctctttcgatgacctccca
ttgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaacttttttta
cttcttgctcattagaaagaaagcatagcaatctaatctaagttttaattacaaaactagatggataaagcggaattaa
ttcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccgccaattttaatcaaagtgggaatattgctga
tagctcattgtccttcactttcactaacagtagcaacggtccgaacctcataacaactcaaacaaattctcaagcgctt
tcacaaccaattgcctcctctaacgttcatgataacttcatgaataatgaaatcacggctagtaaaattgatgatggta
ataattcaaaaccactgtcacctggttggacggaccaaactgcgtataacgcgtttggaatcactacagggatgtttaa
taccactacaatggatgatgtatataactatctattcgatgatgaagataccccaccaaacccaaaaaaagagatcttt
aatacgactcactatagggcgagcgccgccatggagtacccatacgacgtaccagattacgctcatatggccatggagg
ccagtgaattgcctctgtttgaacttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctcc
acctgtagatgaagttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaac
attcctccaccacaagcaaactcttcaagagctagagagattggaaatggctcaaagacgactatggtggacgagatcc
ctatgtcagtgccatcactaatgacgggtttgagtcaagacgatgactttgttccatggttgaatcatcattaatacta
gagggagattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaattta
tccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctcttttttcagttatatcttaac
aggcgacagtccaaatgttgatttatcccagtccgattcatcagggttgtgaagcattttgtcaatggtcgaaatcaca
tcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacgtcaccgtttgtcttttatagtttcgaaatc
tatggtgataccaaatggtgttcccaattcatcgttacgggcgtattttttaccaattgaagtattggaatcgtcaatt
ttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagtatagcggggaagccatcgttattcgatattg
tcgtaacaaatactttgagcggcgctatctgtaatgtactagaatcacacccaatcccccacaagtgatcccccacaca
ccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaa
aacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttgga
aaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttg
aaaatttttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcttcaatttctc
aagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaa
tctaagttttaattacaaaactagatggccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaat
tgggtaccgccaccggcgtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaacc
gtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaaga
agcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccg
gcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagat
cacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagt
ttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaac
ctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgt
tgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcat
aggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttc
aatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacac
tgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaa
gttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgt
caaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacga
taggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaac
atgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaa
gaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgc
ttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgc
gttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagta
tcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtga
agttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggc
gatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagact
ttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatga
tgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaact
gcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactcta
aagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaag
agagatggttaggctcattgagactgcaactgttcctatattcgctgtggatgccggaggctgcatcaatggatggaac
gctaagattgcagagttgacaggtctctcagttgaagaagctatggggaagtctctggtttctgatttaatatacaaag
agaatgaagcaactgtcaataagcttctttctcgtgctttgagaggggacgaggaaaagaatgtggaggttaagctgaa
aactttcagccccgaactacaagggaaagcagtttttgtggttgtgaatgcttgttccagcaaggactacttgaacaac
attgtcggcgtttgttttgttggacaagacgttacgagtcagaaaatcgtaatggataagttcatcaacatacaaggag
attacaaggctattgtacatagcccaaaccctctaatcccgccaatttttgctgctgacgagaacacgtgctgcctgga
atggaacatggcgatggaaaagcttacgggttggtctcgcagtgaagtgattgggaaaatgattgtcggggaagtgttt
gggagctgttgcatgctaaagggtcctgatgctttaaccaagttcatgattgtattgcataatgcgattggtggccaag
atacggataagttccctttcccattctttgaccgcaatgggaagtttgttcaggctctattgactgcaaacaagcgggt
tagcctcgagggaaaggttattggggctttctgtttcttgcaaatcccgagcaccggcgcgagcggcgcgggcggcagc
gaaggcggcggcagcgaaggcggcaccagcggcgcgaccaccggcaaagcgttaacggccaggcaacaagaggtgtttg
atctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttc
cccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcggg
attcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccacttctggcgcaac
agcatattgaaggtcattatcaggtcgatccttccttattcaagccgaatgctgatttcctgctgcgcgtcagcgggat
gtcgatgaaagatatcggcattatggatggtgacttgctggcagtgcataaaactcaggatgtacgtaacggtcaggtc
gttgtcgcacgtattgatgacgaggttaccgttaagcgcctgaaaaaacagggcaataaagtcgaactgttgccagaaa
atagcgagtttaaaccaattgtcgtagatcttcgtcagcagagcttcaccattgaagggctggcggttggggttattcg
caacggcgactggctgaccggttaatactagagggagattgataagacttttctagttgcatatcttttatatttaaat
cttatctattagttaattttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatctaaaa
attcccctcttttttcagttatatcttaacaggcgacagtccaaatgttgatttatcccagtccgattcatcagggttg
tgaagcattttgtcaatggtcgaaatcacatcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacg
tcaccgtttgtcttttatagtttcgaaatctatggtgataccaaatggtgttcccaattcatcgttacgggcgtatttt
ttaccaattgaagtattggaatcgtcaattttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagta
tagcggggaagccatcgttattcgatattgtcgtaacaaatactttgagcggcgctatctgtaatg