LOCUS       BBa_K801075             2208 bp    DNA     linear       22-Dec-2024
DEFINITION  CaDXMT1 expression cassette for yeast
ACCESSION   BBa_K801075
VERSION     BBa_K801075.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2208
                     /label=Cassette
     promoter        1..648
                     /label=Tef2 promoter
     CDS             663..1814
                     /label=CaDXMT1
     misc_feature    1859..2208
                     /label=ADH1 Terminator
     misc_feature    657..662
                     /label=Consensus sequence
     misc_feature    1845..1850
                     /label=Stop codon
     misc_feature    1815..1844
                     /label=Strep-Tag
     misc_feature    663..665
                     /label=Start codon
ORIGIN
        1 ggctacctat attccaccat aacatcaatc atgcggttgc tggtgtattt accaataatg
       61 tttaatgtat atatatatat atatatatgg ggccgtatac ttacatatag tagatgtcaa
      121 gcgtaggcgc ttcccctgcc ggctgtgagg gcgccataac caaggtatct atagaccgcc
      181 aatcagcaaa ctacctccgt acattcatgt tgcacccaca catttataca cccagaccgc
      241 gacaaattac ccataaggtt gtttgtgacg gcgtcgtaca agagaacgtg ggaacttttt
      301 aggctcacca aaaaagaaag aaaaaatacg agttgctgac agaagcctca agaaaaaaaa
      361 aattcttctt cgactatgct ggaggcagag atgatcgagc cggtagttaa ctatatatag
      421 ctaaattggt tccatcacct tcttttctgg tgtcgctcct tctagtgcta tttctggctt
      481 ttcctatttt tttttttcca tttttctttc tctctttcta atatataaat tctcttgcat
      541 tttctatttt tctctctatc tattctactt gtttattccc ttcaaggttt ttttttaagg
      601 agtacttgtt tttagaatat acggtcaacg aactataatt aactaaacta ctagagtaca
      661 caatgtcttt acaagaagtc ttgcatatga acggtggtga aggtgatact tcttacgcta
      721 agaactcttt ctacaacttg ttcttgatca gagtcaagcc aatcttggaa caatgcatcc
      781 aagaattatt gagagccaac ttgccaaaca tcaacaagtg tattaaggtt gctgatttgg
      841 gttgtgcttc tggtccaaat actttgttga ctgttagaga catcgtccaa tccattgata
      901 aggttggtca agaaaagaag aacgaattgg aaagaccaac catccaaatt ttcttgaacg
      961 acttgttcca aaacgacttc aactccgttt ttaagtcctt gccatccttc tacagaaagt
     1021 tggaaaaaga aaacggtaga aagatcggtt cctgtttgat tggtgctatg ccaggttctt
     1081 tttacggtag attattccct gaagaatcca tgcatttctt gcattcttgt tactgcttgc
     1141 actggttgtc tcaagttcca tctggtttgg ttactgaatt gggtatttct gctaacaagg
     1201 gttgcatcta ctcttctaaa gcttcaagac caccaattca aaaggcctac ttggatcaat
     1261 tcactaagga tttcaccact ttcttgagaa tccactccga agaattgatc agtagaggta
     1321 gaatgttgtt gacctggatc tgcaaagaag atgaatttga aaacccaaac tccatcgatt
     1381 tgttggaaat gtccatcaac gatttggtta tcgaaggtca cttagaagaa gaaaagttgg
     1441 actctttcaa cgttccaatc tatgctccat ctaccgaaga agttaagtgc atcgttgaag
     1501 aagaaggttc cttcgaaatc ttgtacttgg aaacctttaa ggttccatac gatgccggtt
     1561 tctctatcga tgatgattat caaggtagat cccactctcc agtttcttgt gatgaacatg
     1621 ctagagctgc tcatgttgct tcagttgtta gatctatttt cgaacctatc gttgcctctc
     1681 atttcggtga agctattatg ccagatttgt cccatagaat tgctaagaat gctgccaagg
     1741 ttttgagatc tggtaagggt ttttacgact ccttgattat ttccttggcc aaaaagccag
     1801 aaaagtccga tgtttctgct tggtcacatc cacaattcga aaagtgatga tactagagag
     1861 ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta
     1921 ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg
     1981 acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
     2041 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat
     2101 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc
     2161 ttattgacca cacctctacc ggccggtcga aattccccta ccctatgt
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