LOCUS BBa_K801075 2208 bp DNA linear 22-Nov-2024 DEFINITION CaDXMT1 expression cassette for yeast ACCESSION BBa_K801075 VERSION BBa_K801075.1 FEATURES Location/Qualifiers misc_feature 1..2208 /label=Cassette promoter 1..648 /label=Tef2 promoter CDS 663..1814 /label=CaDXMT1 misc_feature 1859..2208 /label=ADH1 Terminator misc_feature 657..662 /label=Consensus sequence misc_feature 1845..1850 /label=Stop codon misc_feature 1815..1844 /label=Strep-Tag misc_feature 663..665 /label=Start codon ORIGIN 1 ggctacctat attccaccat aacatcaatc atgcggttgc tggtgtattt accaataatg 61 tttaatgtat atatatatat atatatatgg ggccgtatac ttacatatag tagatgtcaa 121 gcgtaggcgc ttcccctgcc ggctgtgagg gcgccataac caaggtatct atagaccgcc 181 aatcagcaaa ctacctccgt acattcatgt tgcacccaca catttataca cccagaccgc 241 gacaaattac ccataaggtt gtttgtgacg gcgtcgtaca agagaacgtg ggaacttttt 301 aggctcacca aaaaagaaag aaaaaatacg agttgctgac agaagcctca agaaaaaaaa 361 aattcttctt cgactatgct ggaggcagag atgatcgagc cggtagttaa ctatatatag 421 ctaaattggt tccatcacct tcttttctgg tgtcgctcct tctagtgcta tttctggctt 481 ttcctatttt tttttttcca tttttctttc tctctttcta atatataaat tctcttgcat 541 tttctatttt tctctctatc tattctactt gtttattccc ttcaaggttt ttttttaagg 601 agtacttgtt tttagaatat acggtcaacg aactataatt aactaaacta ctagagtaca 661 caatgtcttt acaagaagtc ttgcatatga acggtggtga aggtgatact tcttacgcta 721 agaactcttt ctacaacttg ttcttgatca gagtcaagcc aatcttggaa caatgcatcc 781 aagaattatt gagagccaac ttgccaaaca tcaacaagtg tattaaggtt gctgatttgg 841 gttgtgcttc tggtccaaat actttgttga ctgttagaga catcgtccaa tccattgata 901 aggttggtca agaaaagaag aacgaattgg aaagaccaac catccaaatt ttcttgaacg 961 acttgttcca aaacgacttc aactccgttt ttaagtcctt gccatccttc tacagaaagt 1021 tggaaaaaga aaacggtaga aagatcggtt cctgtttgat tggtgctatg ccaggttctt 1081 tttacggtag attattccct gaagaatcca tgcatttctt gcattcttgt tactgcttgc 1141 actggttgtc tcaagttcca tctggtttgg ttactgaatt gggtatttct gctaacaagg 1201 gttgcatcta ctcttctaaa gcttcaagac caccaattca aaaggcctac ttggatcaat 1261 tcactaagga tttcaccact ttcttgagaa tccactccga agaattgatc agtagaggta 1321 gaatgttgtt gacctggatc tgcaaagaag atgaatttga aaacccaaac tccatcgatt 1381 tgttggaaat gtccatcaac gatttggtta tcgaaggtca cttagaagaa gaaaagttgg 1441 actctttcaa cgttccaatc tatgctccat ctaccgaaga agttaagtgc atcgttgaag 1501 aagaaggttc cttcgaaatc ttgtacttgg aaacctttaa ggttccatac gatgccggtt 1561 tctctatcga tgatgattat caaggtagat cccactctcc agtttcttgt gatgaacatg 1621 ctagagctgc tcatgttgct tcagttgtta gatctatttt cgaacctatc gttgcctctc 1681 atttcggtga agctattatg ccagatttgt cccatagaat tgctaagaat gctgccaagg 1741 ttttgagatc tggtaagggt ttttacgact ccttgattat ttccttggcc aaaaagccag 1801 aaaagtccga tgtttctgct tggtcacatc cacaattcga aaagtgatga tactagagag 1861 ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta 1921 ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg 1981 acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa 2041 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat 2101 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc 2161 ttattgacca cacctctacc ggccggtcga aattccccta ccctatgt //