LOCUS BBa_K801077 6387 bp DNA linear 25-Nov-2024 DEFINITION Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2 -DXMT1-tADH1 ACCESSION BBa_K801077 VERSION BBa_K801077.1 FEATURES Location/Qualifiers misc_feature 1..6387 /label=Cassette1 + Cassette 2+ Cassette 3 promoter 1..648 /label=Tef2 promoter CDS 663..1778 /label=CaXMT1 misc_feature 1823..2172 /label=ADH1 terminator promoter 2181..2631 /label=Tef1 promoter CDS 2644..3777 /label=CaMXMT1 misc_feature 3823..4171 /label=ADH1 terminator promoter 4180..4827 /label=Tef2 promoter CDS 4842..5993 /label=CaDXMT1 misc_feature 6038..6387 /label=ADH1 terminator misc_feature 657..662 /label=Consensus sequence misc_feature 1779..1808 /label=Strep-Tag misc_feature 1809..1814 /label=Stop codon misc_feature 2638..2643 /label=Consensus sequence misc_feature 3778..3807 /label=Strep-Tag misc_feature 3808..3813 /label=Stop codon misc_feature 4836..4841 /label=Consesus sequence misc_feature 5994..6023 /label=Strep-Tag misc_feature 6024..6029 /label=Stop codon misc_feature 663..665 /label=Start codon misc_feature 2644..2646 /label=Start codon misc_feature 4842..4844 /label=Start codon ORIGIN 1 ggctacctat attccaccat aacatcaatc atgcggttgc tggtgtattt accaataatg 61 tttaatgtat atatatatat atatatatgg ggccgtatac ttacatatag tagatgtcaa 121 gcgtaggcgc ttcccctgcc ggctgtgagg gcgccataac caaggtatct atagaccgcc 181 aatcagcaaa ctacctccgt acattcatgt tgcacccaca catttataca cccagaccgc 241 gacaaattac ccataaggtt gtttgtgacg gcgtcgtaca agagaacgtg ggaacttttt 301 aggctcacca aaaaagaaag aaaaaatacg agttgctgac agaagcctca agaaaaaaaa 361 aattcttctt cgactatgct ggaggcagag atgatcgagc cggtagttaa ctatatatag 421 ctaaattggt tccatcacct tcttttctgg tgtcgctcct tctagtgcta tttctggctt 481 ttcctatttt tttttttcca tttttctttc tctctttcta atatataaat tctcttgcat 541 tttctatttt tctctctatc tattctactt gtttattccc ttcaaggttt ttttttaagg 601 agtacttgtt tttagaatat acggtcaacg aactataatt aactaaacta ctagagtaca 661 caatgtcttt acaagaagtc ttgagaatga acggtggtga aggtgatact tcttacgcta 721 aaaactccgc ctacaatcaa ttggttttgg ctaaagttaa gccagtcttg gaacaatgcg 781 tcagagaatt attgagagct aacttgccaa acatcaacaa gtgcattaag gttgctgatt 841 tgggttgtgc ttctggtcca aatactttgt tgactgttag agacatcgtc caatccattg 901 ataaggttgg tcaagaaaag aagaacgaat tggaaagacc aaccatccaa attttcttga 961 acgacttgtt cccaaacgac ttcaactctg tttttaagtt gttgccatcc ttctacagaa 1021 agttggaaaa agaaaacggt agaaagatcg gttcctgttt gattggtgct atgccaggtt 1081 ctttctactc cagattattt cctgaagaat ccatgcattt cttgcactct tgttattgct 1141 tgcaatggtt gtctcaagtt ccatctggtt tggttactga attgggtatt tctaccaaca 1201 agggttccat ctactcttct aaagcttcaa gattgccagt tcaaaaggcc tacttggatc 1261 aattcactaa ggatttcacc acctttttga gaatccactc cgaagaatta ttctcccacg 1321 gtagaatgtt gttgacctgt atatgtaagg gtgttgaatt ggatgctaga aacgccattg 1381 atttgttgga aatggctatc aacgatttgg ttgttgaagg tcacttagaa gaagaaaagt 1441 tggactcttt caacttgcca gtttacattc catctgccga agaagttaag tgcatcgttg 1501 aagaagaagg ttccttcgaa atcttgtact tggaaacttt caaggtcttg tacgatgccg 1561 gtttctctat tgatgatgaa catattaagg ccgaatacgt tgcctcttct gttagagctg 1621 tttacgaacc tattttggct tctcatttcg gtgaagccat tatcccagat atctttcata 1681 gatttgctaa gcacgctgct aaggttttgc cattgggtaa aggtttttac aacaacttga 1741 tcatctcctt ggccaaaaag ccagaaaagt ctgatgtttc tgcttggtca catccacaat 1801 tcgaaaagtg atgatactag agagctttgg acttcttcgc cagaggtttg gtcaagtctc 1861 caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc 1921 aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga 1981 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt 2041 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt 2101 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggccgg tcgaaattcc 2161 cctaccctat gttactagag atcacaccca atcccccaca agtgatcccc cacacaccat 2221 agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac tccgcgcatc 2281 gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt tcttcctcta 2341 gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc 2401 tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc ttgaaaattt 2461 ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt 2521 cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct 2581 tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa actagagtac 2641 acaatgtctt tacaagaagt cttgcacatg aacgaaggtg aaggtgatac ttcttacgct 2701 aagaatgctt cttacaactt ggctttggct aaggttaagc cattcttgga acaatgcatc 2761 agagaattat tgagagccaa cttgccaaac atcaacaagt gtattaaggt tgctgatttg 2821 ggttgtgctt ctggtccaaa tactttgttg actgttagag acatcgtcca atccattgat 2881 aaggttggtc aagaagaaaa gaacgaattg gaaagaccaa ccatccaaat tttcttgaac 2941 gacttgttcc aaaacgactt caactccgtt tttaagttgt tgccatcctt ctacagaaag 3001 ttggaaaaag aaaacggtag aaagatcggt tcctgcttga tttctgctat gccaggttct 3061 ttttacggta gattattccc tgaagaatcc atgcatttct tgcactcttg ttactctgtt 3121 cactggttgt ctcaagttcc atctggtttg gttattgaat tgggtattgg tgctaacaag 3181 ggttccatct attcttctaa aggttgtaga ccaccagttc aaaaggctta cttggatcaa 3241 ttcactaagg acttcaccac tttcttgaga atccactcca aagaattatt ctccagaggt 3301 agaatgttgt tgacctgtat ctgtaaggtt gacgaatttg atgaacctaa cccattggat 3361 ttgttggata tggccattaa cgatttgatc gtcgaaggtt tgttggaaga agaaaagttg 3421 gactccttca acattccatt ctttactcca tctgccgaag aagttaagtg catcgttgaa 3481 gaagaaggtt cttgcgaaat cttgtacttg gaaactttca aggctcatta cgatgctgcc 3541 ttctctattg atgatgatta cccagttaga tcccacgaac aaatcaaagc tgaatacgtt 3601 gcctccttga tcagatctgt ttacgaacct attttggcct ctcatttcgg tgaagctatt 3661 atgccagatt tgtttcacag attggctaaa catgctgcta aggttttaca catgggtaag 3721 ggttgttaca acaacttgat tatctccttg gccaaaaagc cagaaaagtc tgatgtttct 3781 gcttggtcac atccacaatt cgaaaagtga tgatactaga gagctttgga cttcttcgcc 3841 agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac 3901 gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg 3961 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac 4021 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc 4081 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct 4141 accggccggt cgaaattccc ctaccctatg ttactagagg gctacctata ttccaccata 4201 acatcaatca tgcggttgct ggtgtattta ccaataatgt ttaatgtata tatatatata 4261 tatatatggg gccgtatact tacatatagt agatgtcaag cgtaggcgct tcccctgccg 4321 gctgtgaggg cgccataacc aaggtatcta tagaccgcca atcagcaaac tacctccgta 4381 cattcatgtt gcacccacac atttatacac ccagaccgcg acaaattacc cataaggttg 4441 tttgtgacgg cgtcgtacaa gagaacgtgg gaacttttta ggctcaccaa aaaagaaaga 4501 aaaaatacga gttgctgaca gaagcctcaa gaaaaaaaaa attcttcttc gactatgctg 4561 gaggcagaga tgatcgagcc ggtagttaac tatatatagc taaattggtt ccatcacctt 4621 cttttctggt gtcgctcctt ctagtgctat ttctggcttt tcctattttt ttttttccat 4681 ttttctttct ctctttctaa tatataaatt ctcttgcatt ttctattttt ctctctatct 4741 attctacttg tttattccct tcaaggtttt tttttaagga gtacttgttt ttagaatata 4801 cggtcaacga actataatta actaaactac tagagtacac aatgtcttta caagaagtct 4861 tgcatatgaa cggtggtgaa ggtgatactt cttacgctaa gaactctttc tacaacttgt 4921 tcttgatcag agtcaagcca atcttggaac aatgcatcca agaattattg agagccaact 4981 tgccaaacat caacaagtgt attaaggttg ctgatttggg ttgtgcttct ggtccaaata 5041 ctttgttgac tgttagagac atcgtccaat ccattgataa ggttggtcaa gaaaagaaga 5101 acgaattgga aagaccaacc atccaaattt tcttgaacga cttgttccaa aacgacttca 5161 actccgtttt taagtccttg ccatccttct acagaaagtt ggaaaaagaa aacggtagaa 5221 agatcggttc ctgtttgatt ggtgctatgc caggttcttt ttacggtaga ttattccctg 5281 aagaatccat gcatttcttg cattcttgtt actgcttgca ctggttgtct caagttccat 5341 ctggtttggt tactgaattg ggtatttctg ctaacaaggg ttgcatctac tcttctaaag 5401 cttcaagacc accaattcaa aaggcctact tggatcaatt cactaaggat ttcaccactt 5461 tcttgagaat ccactccgaa gaattgatca gtagaggtag aatgttgttg acctggatct 5521 gcaaagaaga tgaatttgaa aacccaaact ccatcgattt gttggaaatg tccatcaacg 5581 atttggttat cgaaggtcac ttagaagaag aaaagttgga ctctttcaac gttccaatct 5641 atgctccatc taccgaagaa gttaagtgca tcgttgaaga agaaggttcc ttcgaaatct 5701 tgtacttgga aacctttaag gttccatacg atgccggttt ctctatcgat gatgattatc 5761 aaggtagatc ccactctcca gtttcttgtg atgaacatgc tagagctgct catgttgctt 5821 cagttgttag atctattttc gaacctatcg ttgcctctca tttcggtgaa gctattatgc 5881 cagatttgtc ccatagaatt gctaagaatg ctgccaaggt tttgagatct ggtaagggtt 5941 tttacgactc cttgattatt tccttggcca aaaagccaga aaagtccgat gtttctgctt 6001 ggtcacatcc acaattcgaa aagtgatgat actagagagc tttggacttc ttcgccagag 6061 gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa 6121 agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt 6181 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat 6241 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt 6301 aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg 6361 gccggtcgaa attcccctac cctatgt //