BBa_K806001 1 BBa_K806001 iciA-inhibitor of chromosomal initiation of replication 2012-09-23T11:00:00Z 2015-05-08T01:13:25Z We clone this gene from E.coli strain BL21 with primer iciA-F and iciA-R iciA-F sequence:GCTCTAGAGCATGAAACGCCCGGACTACAGAAC iciA-R sequence:GGACTAGTCCTTAATCCTGACGAAGGACTTTGTGAC iciA sequence:(GenBank: CP001509.3) 1 atgaaacgcc cggactacag aacattacag gcactggatg cggtgatacg tgaacgagga 61 tttgagcgcg cggcacaaaa gctgtgcatt acacaatcag ccgtctcaca gcgcattaag 121 caactggaaa atatgttcgg gcagccgctg ttggtgcgta ccgtaccgcc gcgcccgacg 181 gaacaagggc aaaaactgct ggcactgctg cgccaggtgg agttgctgga agaagagtgg 241 ctgggcgatg aacaaaccgg ttcgactccg ctgctgcttt cactggcggt caacgccgac 301 agtctggcga cgtggttgct tcctgcactg gctcctgtgt tggctgattc gcctatccgc 361 ctcaacttgc aggtagaaga tgaaacccgc actcaggaac gtctgcgccg cggcgaagtg 421 gtcggcgcgg tgagtattca acatcaggcg ctgccgagtt gtcttgtcga taaacttggt 481 gcgctcgact atctgttcgt cagctcaaaa ccctttgccg aaaaatattt ccctaacggc 541 gtaacgcgtt cggcattact gaaagcgcca gtggtcgcgt ttgaccatct tgacgatatg 601 caccaggcct ttttgcagca aaacttcgat ctgcctccag gcagcgtgcc ctgccatatc 661 gttaattctt cagaagcgtt cgtacaactt gctcgccagg gcaccacctg ctgtatgatc 721 ccgcacctgc aaatcgagaa agagctggcc agcggtgaac tgattgactt aacgcctggg 781 ctatttcaac gacggatgct ctactggcac cgctttgctc ctgaaagccg catgatgcgt 841 aaagtcactg atgcgttact cgattatggt cacaaagtcc ttcgtcagga ttaa We find a better way to slow down the cell cycle of E.coli,by stop the initiation of DNA replication. iciA is ???the gene encoding the protein that binds the three 13-mers in the origin (oriC) of Escherichia coli to block initiation of replication in vitro??? we cloned and overexpressed it to slow down certain kind of cells in a mix quorum system, in order to maintain a more stable system and achieve better energy partition. false false _1063_ 0 11692 9 It's complicated false 1. Add restriction enzyme in the front term of PCR primers 2. Using genomic DNA extraction as a template of PCR we have better effect false Chang Ye annotation2203338 1 iciA range2203338 1 1 894 BBa_K806001_sequence 1 atgaaacgcccggactacagaacattacaggcactggatgcggtgatacgtgaacgaggatttgagcgcgcggcacaaaagctgtgcattacacaatcagccgtctcacagcgcattaagcaactggaaaatatgttcgggcagccgctgttggtgcgtaccgtaccgccgcgcccgacggaacaagggcaaaaactgctggcactgctgcgccaggtggagttgctggaagaagagtggctgggcgatgaacaaaccggttcgactccgctgctgctttcactggcggtcaacgccgacagtctggcgacgtggttgcttcctgcactggctcctgtgttggctgattcgcctatccgcctcaacttgcaggtagaagatgaaacccgcactcaggaacgtctgcgccgcggcgaagtggtcggcgcggtgagtattcaacatcaggcgctgccgagttgtcttgtcgataaacttggtgcgctcgactatctgttcgtcagctcaaaaccctttgccgaaaaatatttccctaacggcgtaacgcgttcggcattactgaaagcgccagtggtcgcgtttgaccatcttgacgatatgcaccaggcctttttgcagcaaaacttcgatctgcctccaggcagcgtgccctgccatatcgttaattcttcagaagcgttcgtacaacttgctcgccagggcaccacctgctgtatgatcccgcacctgcaaatcgagaaagagctggccagcggtgaactgattgacttaacgcctgggctatttcaacgacggatgctctactggcaccgctttgctcctgaaagccgcatgatgcgtaaagtcactgatgcgttactcgattatggtcacaaagtccttcgtcaggattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z