BBa_K807002
1
LEV
LEV: LexA DNA Binding Domain - Human Estrogen Receptor - VP16 Activating Domain
2012-09-27T11:00:00Z
2015-05-08T01:13:25Z
LexA DBD - E.coli
hER - Human
VP16 - Herpes virus
This part is a synthetic transcriptional activator with specificity for the LexA operator site. It is inactive in its native form but can be activated by the addition of Beta-Estradiol. The Beta-Estradiol acts to dimerize the activator.
true
false
_1064_
0
9288
9
Discontinued
false
LexA DBD was yeast optimized and synthesized by IDT. We had to ensure that LexA DBD and hER did not have stop codons in order for the fusion protein to be built.
false
Ian Roney
annotation2203860
1
VP16
range2203860
1
1136
1339
annotation2203859
1
hER
range2203859
1
253
1135
annotation2203858
1
LexA DBD
range2203858
1
1
252
BBa_K807002_sequence
1
atgaaggctttgactgctagacaacaagaagttttcgacttgatcagagaccacatctctcaaactggtatgccaccaactagagctgaaatcgctcaaagattgggtttcagatctccaaacgctgctgaagaacacttgaaggctttggctagaaagggtgttatcgaaatcgtttctggtgcttctaggggtatcagattgttgcaagaagaagaagaaggtttgccattggttggtagagttgctgctagctggagacatgagagctgccaacctttggccaagcccgctcatgatcaaacgctctaagaagaacagcctggccttgtccctgacggccgaccagatggtcagtgccttgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaagcttcgatgatgggcttactgaccaacctggcagacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggatttgaccctccatgatcaggtccacctactagaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagcacccagtgaagctactgtttgctcctaacttgctcttggacaggaaccagggaaaatgtgtagagggcatggtggagatcttcgacatgctgctggctacatcatctcggttccgcatgatgaatctacagggagaggagtttgtgtgcctcaaatctattattttgcttaattctggagtgtacacatttctgtccagcaccctgaagtctctggaagagaaggaccatatccaccgagtcctggacaagatcacagacactttgatccacctgatggccaaggcaggcctgaccctacagcagcagcaccagcggctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgtacagcatgaagtgcaagaacgtggtgcccctctatgacctgctgctggagatgctggacgcccaccgcctacatgcgcccactagccgtggaggggcatccgtggaggagacggaccaaagccacttggccactgcgggctctacttcatcggagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z