BBa_K807002 1 LEV LEV: LexA DNA Binding Domain - Human Estrogen Receptor - VP16 Activating Domain 2012-09-27T11:00:00Z 2015-05-08T01:13:25Z LexA DBD - E.coli hER - Human VP16 - Herpes virus This part is a synthetic transcriptional activator with specificity for the LexA operator site. It is inactive in its native form but can be activated by the addition of Beta-Estradiol. The Beta-Estradiol acts to dimerize the activator. true false _1064_ 0 9288 9 Discontinued false LexA DBD was yeast optimized and synthesized by IDT. We had to ensure that LexA DBD and hER did not have stop codons in order for the fusion protein to be built. false Ian Roney annotation2203859 1 hER range2203859 1 253 1135 annotation2203860 1 VP16 range2203860 1 1136 1339 annotation2203858 1 LexA DBD range2203858 1 1 252 BBa_K807002_sequence 1 atgaaggctttgactgctagacaacaagaagttttcgacttgatcagagaccacatctctcaaactggtatgccaccaactagagctgaaatcgctcaaagattgggtttcagatctccaaacgctgctgaagaacacttgaaggctttggctagaaagggtgttatcgaaatcgtttctggtgcttctaggggtatcagattgttgcaagaagaagaagaaggtttgccattggttggtagagttgctgctagctggagacatgagagctgccaacctttggccaagcccgctcatgatcaaacgctctaagaagaacagcctggccttgtccctgacggccgaccagatggtcagtgccttgttggatgctgagccccccatactctattccgagtatgatcctaccagacccttcagtgaagcttcgatgatgggcttactgaccaacctggcagacagggagctggttcacatgatcaactgggcgaagagggtgccaggctttgtggatttgaccctccatgatcaggtccacctactagaatgtgcctggctagagatcctgatgattggtctcgtctggcgctccatggagcacccagtgaagctactgtttgctcctaacttgctcttggacaggaaccagggaaaatgtgtagagggcatggtggagatcttcgacatgctgctggctacatcatctcggttccgcatgatgaatctacagggagaggagtttgtgtgcctcaaatctattattttgcttaattctggagtgtacacatttctgtccagcaccctgaagtctctggaagagaaggaccatatccaccgagtcctggacaagatcacagacactttgatccacctgatggccaaggcaggcctgaccctacagcagcagcaccagcggctggcccagctcctcctcatcctctcccacatcaggcacatgagtaacaaaggcatggagcatctgtacagcatgaagtgcaagaacgtggtgcccctctatgacctgctgctggagatgctggacgcccaccgcctacatgcgcccactagccgtggaggggcatccgtggaggagacggaccaaagccacttggccactgcgggctctacttcatcggagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z