BBa_K808030 1 BBa_K808030 RBS-PhoA-His6tag-pNBEst13-Myctag-EstA 2012-09-01T11:00:00Z 2015-05-08T01:13:26Z This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR. RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor. false false _1065_ 0 11792 9 In stock false We got it synthesized by GENEART but added the RBS via PCR. false Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling annotation2182935 1 BBa_K808026 range2182935 1 104 1573 annotation2182936 1 myctag range2182936 1 1574 1603 annotation2182937 1 BBa_K808027 range2182937 1 1604 3037 annotation2182926 1 BBa_B0029 range2182926 1 2 16 annotation2182934 1 BBa_K808028 range2182934 1 23 85 annotation2182940 1 start range2182940 1 23 25 annotation2182939 1 stop range2182939 1 3041 3043 annotation2182933 1 6 histag range2182933 1 86 103 annotation2182938 1 stop range2182938 1 3038 3040 BBa_K808030_sequence 1 gttcacacaggaaacctactagatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcgcatcaccaccatcatcacatgtatgatacaactgtcgaaacacgcttcggaaagctgaaaggcagagcggaaaacggagtccgtatctttaaaggcgttccatacgcaaaacctcccgtcggcgacctaaggtttcgggaaccgcagcgaatggaggcctgggaaggtgagctggatgcttttcaatttggcccggtttgtccgcagcctgatggggtattgcctgagtcagcgggggttcaaaagtctgaggattgcctttatttaaatgtgtacgcacccgaagaggcggacggggatctgcctgttatggtgtggattcatgggggcgctttttatcgcggcgccggaagtgaaccgctctatgacgggactcagcttgcaaagcagggaaaggtgatcgtggtcaccatcaattatcgcctcggtccgttcggttttttgcatctatcctcaattgatgattcctacagcagcaatcttggcctgctggatcaaatcgcggctttagagtgggtgaaagacaatatcgctttctttggcggtgaccgtcatcacattacggtttttggagagtcggcgggatcgatgagctgcgcttcgcttttggcaatgccgaaagcaaaggggctttttcaacaggccattatggaaagcggggcttccgcaactatgtccgataagttggcgaaagccgcagcagaaagattcttaaggattctcgatattgatcatcatcatctggagcgccttcatgatgtatctgatcaagaacttcttgaagccgccgatcagctgcgcactttaatgggagaaaatatttttgaattgatttttctgcctgcgcttgacgaaaaaaccttgccgctgaagccggaggtcgccgtcgcaaaaggcgcggcaaaagagatcaatctattaatcggaacaaaccgtgatgaaggcgtcttgttttttccctctgattcggatcttttgcctgagagcaagatcaacgagattttagaagaatacatgggtaaagaggccgccgaagccgcctcctctctgtatccgaggtcattggaaggccatgttgatatgatgacagatctgatcttttggcatccgtctgttgtgttcgcttcggctcaatcacgatatgcatctgtctttatgtaccggtttgactggcatgcggattcagagcagccgccgttcaacaaagctgcgcacggcttagagattccgtttgtatttggaaatatggacattttggaacagctgacaggtacgaaggccggtgaagaagcgcagctgcttgctgaacagatccaggctgcctgggtgtcttttgcccgatccggaaatccgagcaccgatgatgtcagctggcctgattatgatgaagattcacggaaaacgctgatttttgatcaagaggtcgcagttgaaagcgatccttattcagataagagaaagatgttgacagccccgaacccgcagattgaacaaaaactaataagcgaagaagacctagctccttcgccctattcgacgctggtggtgttcggcgacgtccaggcccaacaagccgccggtcgcctggtggatagcgtgcaggccctacaacaggccggcgcgcgctacatcgtggtctggctgttgcccgacctgggcctgaccccggctaccttcggtggtcccttgcagcctttcgccagccaactcagcggcacgttcaacgccgagctgaccgcccagttgagccaggccggcgccaacgtcattccgttgaacatcccgctgctgctcaaggaaggcatggccaacccggcttccttcggcctggccgccgaccagaacctgatcggcacctgtttcagcggcaacggctgcaccatgaacccgacctacgggatcaacggcagcacgcccgacccgagcaaattgctgttcaacgacagcgtgcacccgaccatcaccggccagcgcctgatcgccgactacacctattcgctgctgtcggcgccctgggagctgaccctgctgccggaaatggcccacggcaccctgcgtgcctaccaggacgaactgcgcagccagtggcaggcggactgggagaactggcagaacgtcggccagtggcgcggcttcgtcggcggcggtggccagcgcctggacttcgactcccaggacagcgccgccagcggcgacggcaacggctacaacctgacccttggtggcagctaccgcatcgacgaggcctggcgcgccggggtcgccgccggtttctaccggcagaagctggaagccggcgccaaggattccgactaccggatgaacagctacatggccagcgccttcgtgcagtaccaggaaaaccgctggtgggccgacgcggcgttgaccggcggctacctcgactacgacgacctgaagcgcaagttcgccctgggcggcggcgagcgcagcgagaaaggcgacaccaacggccacctgtgggcgttcagcgcgcgcctgggctacgacattgcccagcaggccgacagtccctggcacctgtcgccgttcgtcagcgccgactatgcacgggtcgaggtcgacggctattccgagaagggcgccagcgccaccgcgctcgactacgacgaccagaagcgcagctcgaagcgcctgggcgccggcctgcaaggcaagtacgcgttcggcagcgatacccagctgttcgccgagtacgcccacgaacgtgagtacgaggacgacacccaggacctgaccatgtccctcaacagcctgccgggcaatcgcttcaccctcgaaggctacaccccgcaggaccatctcaaccgtgtttcactcggcttcagccagaagctggcgccggagctgtcgctgcgcggcggctacaactggcgcaagggcgaggacgatacccagcagagcgtcagcctggcgctgagcctggacttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z