LOCUS BBa_K808032 4261 bp DNA linear 25-Nov-2024 DEFINITION Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA ACCESSION BBa_K808032 VERSION BBa_K808032.1 FEATURES Location/Qualifiers CDS 1..879 /label= AraC misc_feature 909..924 /label=ara O2 misc_feature 1066..1077 /label=ara O1 promoter 1153..1181 /label=ara promotor misc_feature 1211..1218 /label=scar RBS 1220..1234 /label=BBa_B0029 misc_feature 1304..1321 /label=6histag CDS 1241..1303 /label=BBa_K808028 CDS 1322..2791 /label=BBa_K808026 misc_feature 2792..2821 /label=myctag CDS 2822..4255 /label=BBa_K808027 misc_feature 4256..4258 /label=stop misc_feature 4259..4261 /label=stop ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 tgggctaact atactagagt tcacacagga aacctactag atgaaacaaa gcactattgc 1261 actggcactc ttaccgttac tgtttacccc tgtgacaaaa gcgcatcacc accatcatca 1321 catgtatgat acaactgtcg aaacacgctt cggaaagctg aaaggcagag cggaaaacgg 1381 agtccgtatc tttaaaggcg ttccatacgc aaaacctccc gtcggcgacc taaggtttcg 1441 ggaaccgcag cgaatggagg cctgggaagg tgagctggat gcttttcaat ttggcccggt 1501 ttgtccgcag cctgatgggg tattgcctga gtcagcgggg gttcaaaagt ctgaggattg 1561 cctttattta aatgtgtacg cacccgaaga ggcggacggg gatctgcctg ttatggtgtg 1621 gattcatggg ggcgcttttt atcgcggcgc cggaagtgaa ccgctctatg acgggactca 1681 gcttgcaaag cagggaaagg tgatcgtggt caccatcaat tatcgcctcg gtccgttcgg 1741 ttttttgcat ctatcctcaa ttgatgattc ctacagcagc aatcttggcc tgctggatca 1801 aatcgcggct ttagagtggg tgaaagacaa tatcgctttc tttggcggtg accgtcatca 1861 cattacggtt tttggagagt cggcgggatc gatgagctgc gcttcgcttt tggcaatgcc 1921 gaaagcaaag gggctttttc aacaggccat tatggaaagc ggggcttccg caactatgtc 1981 cgataagttg gcgaaagccg cagcagaaag attcttaagg attctcgata ttgatcatca 2041 tcatctggag cgccttcatg atgtatctga tcaagaactt cttgaagccg ccgatcagct 2101 gcgcacttta atgggagaaa atatttttga attgattttt ctgcctgcgc ttgacgaaaa 2161 aaccttgccg ctgaagccgg aggtcgccgt cgcaaaaggc gcggcaaaag agatcaatct 2221 attaatcgga acaaaccgtg atgaaggcgt cttgtttttt ccctctgatt cggatctttt 2281 gcctgagagc aagatcaacg agattttaga agaatacatg ggtaaagagg ccgccgaagc 2341 cgcctcctct ctgtatccga ggtcattgga aggccatgtt gatatgatga cagatctgat 2401 cttttggcat ccgtctgttg tgttcgcttc ggctcaatca cgatatgcat ctgtctttat 2461 gtaccggttt gactggcatg cggattcaga gcagccgccg ttcaacaaag ctgcgcacgg 2521 cttagagatt ccgtttgtat ttggaaatat ggacattttg gaacagctga caggtacgaa 2581 ggccggtgaa gaagcgcagc tgcttgctga acagatccag gctgcctggg tgtcttttgc 2641 ccgatccgga aatccgagca ccgatgatgt cagctggcct gattatgatg aagattcacg 2701 gaaaacgctg atttttgatc aagaggtcgc agttgaaagc gatccttatt cagataagag 2761 aaagatgttg acagccccga acccgcagat tgaacaaaaa ctaataagcg aagaagacct 2821 agctccttcg ccctattcga cgctggtggt gttcggcgac gtccaggccc aacaagccgc 2881 cggtcgcctg gtggatagcg tgcaggccct acaacaggcc ggcgcgcgct acatcgtggt 2941 ctggctgttg cccgacctgg gcctgacccc ggctaccttc ggtggtccct tgcagccttt 3001 cgccagccaa ctcagcggca cgttcaacgc cgagctgacc gcccagttga gccaggccgg 3061 cgccaacgtc attccgttga acatcccgct gctgctcaag gaaggcatgg ccaacccggc 3121 ttccttcggc ctggccgccg accagaacct gatcggcacc tgtttcagcg gcaacggctg 3181 caccatgaac ccgacctacg ggatcaacgg cagcacgccc gacccgagca aattgctgtt 3241 caacgacagc gtgcacccga ccatcaccgg ccagcgcctg atcgccgact acacctattc 3301 gctgctgtcg gcgccctggg agctgaccct gctgccggaa atggcccacg gcaccctgcg 3361 tgcctaccag gacgaactgc gcagccagtg gcaggcggac tgggagaact ggcagaacgt 3421 cggccagtgg cgcggcttcg tcggcggcgg tggccagcgc ctggacttcg actcccagga 3481 cagcgccgcc agcggcgacg gcaacggcta caacctgacc cttggtggca gctaccgcat 3541 cgacgaggcc tggcgcgccg gggtcgccgc cggtttctac cggcagaagc tggaagccgg 3601 cgccaaggat tccgactacc ggatgaacag ctacatggcc agcgccttcg tgcagtacca 3661 ggaaaaccgc tggtgggccg acgcggcgtt gaccggcggc tacctcgact acgacgacct 3721 gaagcgcaag ttcgccctgg gcggcggcga gcgcagcgag aaaggcgaca ccaacggcca 3781 cctgtgggcg ttcagcgcgc gcctgggcta cgacattgcc cagcaggccg acagtccctg 3841 gcacctgtcg ccgttcgtca gcgccgacta tgcacgggtc gaggtcgacg gctattccga 3901 gaagggcgcc agcgccaccg cgctcgacta cgacgaccag aagcgcagct cgaagcgcct 3961 gggcgccggc ctgcaaggca agtacgcgtt cggcagcgat acccagctgt tcgccgagta 4021 cgcccacgaa cgtgagtacg aggacgacac ccaggacctg accatgtccc tcaacagcct 4081 gccgggcaat cgcttcaccc tcgaaggcta caccccgcag gaccatctca accgtgtttc 4141 actcggcttc agccagaagc tggcgccgga gctgtcgctg cgcggcggct acaactggcg 4201 caagggcgag gacgataccc agcagagcgt cagcctggcg ctgagcctgg acttctaata 4261 a //