BBa_K808032 1 BBa_K808032 Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA 2012-09-03T11:00:00Z 2015-05-08T01:13:26Z assembled GENEART synthesized product(BBa_K808030)and cloned regulatory unit (BBa_K808000), deriving from an expression system (pBAD-vector) Released HQ 2013 This a a composite of 2 different BioBricks by an standard assembly, forming a scar in between. It contains the following units: AraC-AraO2-AraO1-AraPromo(PBAD)- RBS-PhoA-His6tag-pNBEst13-Myctag-EstA which is similiar to BBa_K808000 & BBa_K808030 Its a regulatory system, being induced by adding 1 to 2% of mw% L-Arabinose. It is made for regulating the cell surface Expression of our PET cleaving enzyme pNB-Est13, which is anchored c-terminal to EstA. false false _1065_ 0 12529 9 In stock false We were not able to add this part as an composite due to missing links and unedited registry pages. Necessary informations are featured in the sequence. false Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling annotation2183173 1 myctag range2183173 1 2792 2821 annotation2183170 1 6histag range2183170 1 1304 1321 annotation2183166 1 ara O1 range2183166 1 1066 1077 annotation2183175 1 stop range2183175 1 4256 4258 annotation2183171 1 BBa_K808028 range2183171 1 1241 1303 annotation2183165 1 ara O2 range2183165 1 909 924 annotation2183167 1 ara promotor range2183167 1 1153 1181 annotation2183169 1 BBa_B0029 range2183169 1 1220 1234 annotation2183164 1 AraC range2183164 1 1 879 annotation2183168 1 scar range2183168 1 1211 1218 annotation2183172 1 BBa_K808026 range2183172 1 1322 2791 annotation2183174 1 BBa_K808027 range2183174 1 2822 4255 annotation2183176 1 stop range2183176 1 4259 4261 BBa_K808032_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaactatactagagttcacacaggaaacctactagatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcgcatcaccaccatcatcacatgtatgatacaactgtcgaaacacgcttcggaaagctgaaaggcagagcggaaaacggagtccgtatctttaaaggcgttccatacgcaaaacctcccgtcggcgacctaaggtttcgggaaccgcagcgaatggaggcctgggaaggtgagctggatgcttttcaatttggcccggtttgtccgcagcctgatggggtattgcctgagtcagcgggggttcaaaagtctgaggattgcctttatttaaatgtgtacgcacccgaagaggcggacggggatctgcctgttatggtgtggattcatgggggcgctttttatcgcggcgccggaagtgaaccgctctatgacgggactcagcttgcaaagcagggaaaggtgatcgtggtcaccatcaattatcgcctcggtccgttcggttttttgcatctatcctcaattgatgattcctacagcagcaatcttggcctgctggatcaaatcgcggctttagagtgggtgaaagacaatatcgctttctttggcggtgaccgtcatcacattacggtttttggagagtcggcgggatcgatgagctgcgcttcgcttttggcaatgccgaaagcaaaggggctttttcaacaggccattatggaaagcggggcttccgcaactatgtccgataagttggcgaaagccgcagcagaaagattcttaaggattctcgatattgatcatcatcatctggagcgccttcatgatgtatctgatcaagaacttcttgaagccgccgatcagctgcgcactttaatgggagaaaatatttttgaattgatttttctgcctgcgcttgacgaaaaaaccttgccgctgaagccggaggtcgccgtcgcaaaaggcgcggcaaaagagatcaatctattaatcggaacaaaccgtgatgaaggcgtcttgttttttccctctgattcggatcttttgcctgagagcaagatcaacgagattttagaagaatacatgggtaaagaggccgccgaagccgcctcctctctgtatccgaggtcattggaaggccatgttgatatgatgacagatctgatcttttggcatccgtctgttgtgttcgcttcggctcaatcacgatatgcatctgtctttatgtaccggtttgactggcatgcggattcagagcagccgccgttcaacaaagctgcgcacggcttagagattccgtttgtatttggaaatatggacattttggaacagctgacaggtacgaaggccggtgaagaagcgcagctgcttgctgaacagatccaggctgcctgggtgtcttttgcccgatccggaaatccgagcaccgatgatgtcagctggcctgattatgatgaagattcacggaaaacgctgatttttgatcaagaggtcgcagttgaaagcgatccttattcagataagagaaagatgttgacagccccgaacccgcagattgaacaaaaactaataagcgaagaagacctagctccttcgccctattcgacgctggtggtgttcggcgacgtccaggcccaacaagccgccggtcgcctggtggatagcgtgcaggccctacaacaggccggcgcgcgctacatcgtggtctggctgttgcccgacctgggcctgaccccggctaccttcggtggtcccttgcagcctttcgccagccaactcagcggcacgttcaacgccgagctgaccgcccagttgagccaggccggcgccaacgtcattccgttgaacatcccgctgctgctcaaggaaggcatggccaacccggcttccttcggcctggccgccgaccagaacctgatcggcacctgtttcagcggcaacggctgcaccatgaacccgacctacgggatcaacggcagcacgcccgacccgagcaaattgctgttcaacgacagcgtgcacccgaccatcaccggccagcgcctgatcgccgactacacctattcgctgctgtcggcgccctgggagctgaccctgctgccggaaatggcccacggcaccctgcgtgcctaccaggacgaactgcgcagccagtggcaggcggactgggagaactggcagaacgtcggccagtggcgcggcttcgtcggcggcggtggccagcgcctggacttcgactcccaggacagcgccgccagcggcgacggcaacggctacaacctgacccttggtggcagctaccgcatcgacgaggcctggcgcgccggggtcgccgccggtttctaccggcagaagctggaagccggcgccaaggattccgactaccggatgaacagctacatggccagcgccttcgtgcagtaccaggaaaaccgctggtgggccgacgcggcgttgaccggcggctacctcgactacgacgacctgaagcgcaagttcgccctgggcggcggcgagcgcagcgagaaaggcgacaccaacggccacctgtgggcgttcagcgcgcgcctgggctacgacattgcccagcaggccgacagtccctggcacctgtcgccgttcgtcagcgccgactatgcacgggtcgaggtcgacggctattccgagaagggcgccagcgccaccgcgctcgactacgacgaccagaagcgcagctcgaagcgcctgggcgccggcctgcaaggcaagtacgcgttcggcagcgatacccagctgttcgccgagtacgcccacgaacgtgagtacgaggacgacacccaggacctgaccatgtccctcaacagcctgccgggcaatcgcttcaccctcgaaggctacaccccgcaggaccatctcaaccgtgtttcactcggcttcagccagaagctggcgccggagctgtcgctgcgcggcggctacaactggcgcaagggcgaggacgatacccagcagagcgtcagcctggcgctgagcctggacttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z