LOCUS       BBa_K808033             2298 bp    DNA     linear       25-Nov-2024
DEFINITION  araC-Pbad regulated XylE - catechol 2,3-dioxygenase from P.putida 
            with terminator
ACCESSION   BBa_K808033
VERSION     BBa_K808033.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     RBS             1218..1229
                     /label=BBa_J61100
     RBS             1251..1256
                     /label=rbs
     CDS             1264..2187
                     /label=cds
     misc_feature    1238..2195
                     /label=BBa_J33204
     stem_loop       2211..2230
                     /label=T7 TE
     misc_feature    2237..2237
                     /label=stop
     polyA_site      2231..2244
                     /label=polya
     misc_feature    2204..2244
                     /label=BBa_B0012
     terminator      2204..2244
                     /label=BBa_B0012
     stem_loop       2265..2287
                     /label=stem_loop
     misc_feature    2253..2298
                     /label=BBa_B0011
     terminator      2253..2298
                     /label=BBa_B0011
     terminator      2204..2298
                     /label=BBa_B0014
     CDS             1238..2298
                     /label=BBa_K316003
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaaa gaggggacat actagagctc atgaactatg aagaggtgac
     1261 gtcatgaaca aaggtgtaat gcgaccgggc catgtgcagc tgcgtgtact ggacatgagc
     1321 aaggccctgg aacactacgt cgagttgctg ggcctgatcg agatggaccg tgacgaccag
     1381 ggccgtgtct atctgaaggc ttggaccgaa gtggataagt tttccctggt gctacgcgag
     1441 gctgacgagc cgggcatgga ttttatgggt ttcaaggttg tggatgagga tgctctccgg
     1501 caactggagc gggatctgat ggcatatggc tgtgccgttg agcagctacc cgcaggtgaa
     1561 ctgaacagtt gtggccggcg cgtgcgcttc caggccccct ccgggcatca cttcgagttg
     1621 tatgcagaca aggaatatac tggaaagtgg ggtttgaatg acgtcaatcc cgaggcatgg
     1681 ccgcgcgatc tgaaaggtat ggcggctgtg cgtttcgacc acgccctcat gtatggcgac
     1741 gaattgccgg cgacctatga cctgttcacc aaggtgctcg gtttctatct ggccgaacag
     1801 gtgctggacg aaaatggcac gcgcgtcgcc cagtttctca gtctgtcgac caaggcccac
     1861 gacgtggcct tcattcacca tccggaaaaa ggccgcctcc atcatgtgtc cttccacctc
     1921 gaaacctggg aagacttgct tcgcgccgcc gacctgatct ccatgaccga cacatctatc
     1981 gatatcggcc caacccgcca cggcctcact cacggcaaga ccatctactt cttcgacccg
     2041 tccggtaacc gcaacgaagt gttctgcggg ggagattaca actacccgga ccacaaaccg
     2101 gtgacctgga ccaccgacca gctgggcaag gcgatctttt accacgaccg cattctcaac
     2161 gaacgattca tgaccgtgct gacctgatgg tccggtacta gagtcacact ggctcacctt
     2221 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat
     2281 ccggcttttt tattattt
//