BBa_K808033
type: ComponentDefinition
persistent identity: BBa K808033
display id: BBa_K808033
version: 1
was generated by: iGEM to SBOL conversion
description: araC-Pbad regulated XylE - catechol 2,3-dioxygenase from P.putida with terminator
created: 2012-09-05T11:00:00Z
modified: 2015-05-08T01:13:26Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 12529
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J61100
component: BBa_K808000
component: BBa_K316003
sequence annotation: BBa_K808000
sequence annotation: BBa_J61100
sequence annotation: BBa_K316003
sequence: https://synbiohub.org/public/igem/BBa_K808033_sequence/1

BBa_J61100
type: Component
persistent identity: component2201323
display id: component2201323
version: 1
top level: BBa_K808033
definition: BBa_J61100
access: public

BBa_K808000
type: Component
persistent identity: component2201322
display id: component2201322
version: 1
top level: BBa_K808033
definition: Ara
access: public

BBa_K316003
type: Component
persistent identity: component2201335
display id: component2201335
version: 1
top level: BBa_K808033
definition: BBa_K316003
access: public

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2201322
display id: annotation2201322
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201322/range2201322/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K808033/annotation2201322/range2201322/1
type: Range
persistent identity: range2201322
display id: range2201322
version: 1
top level: BBa_K808033
direction: direction/reverse
start: 1
end: 1209
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2201323
display id: annotation2201323
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201323/range2201323/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K808033/annotation2201323/range2201323/1
type: Range
persistent identity: range2201323
display id: range2201323
version: 1
top level: BBa_K808033
direction: direction/reverse
start: 1218
end: 1229
orientation: inline

BBa_K316003
type: SequenceAnnotation
persistent identity: annotation2201335
display id: annotation2201335
version: 1
top level: BBa_K808033
location: https://synbiohub.org/public/igem/BBa_K808033/annotation2201335/range2201335/1
component: BBa_K316003

https://synbiohub.org/public/igem/BBa_K808033/annotation2201335/range2201335/1
type: Range
persistent identity: range2201335
display id: range2201335
version: 1
top level: BBa_K808033
direction: direction/reverse
start: 1238
end: 2298
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_K316003
type: ComponentDefinition
persistent identity: BBa K316003
display id: BBa_K316003
version: 1
was generated by: iGEM to SBOL conversion
description: XylE - catechol 2,3-dioxygenase from P.putida with terminator
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: XylE was obtained from the registry <bbpart>BBa_K118021</bbpart>, the terminator is <bbpart>BBa_B0014</bbpart>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Catechol or catechol 2,3-dioxygenases + O(2) is converted by a ring cleavage into 2-hydroxymuconate semialdehyde which is the toxic and bright yellow-coloured substrate. This is a key enzyme in many (soil) bacterial species used for the degradation of aromatic compounds. The enzyme used here itself originating from Pseudomonas putida is a homotetramer of C230 monomers. The tetramerization interactions position a ferrous ion critical for enzymatic activity. It has been deduced that intersubunit interaction is essential to produce a functioning enzyme after performing N and C terminal modifications on the monomer. Coming together the subunits generate an active site. The reaction itself takes place within seconds after the addition by Pasteur pipette or spraying of catechol at a 100mM stock solution diluted with DDH20 (used by our lab.) The toxic byproduct is thought to interfere with cell wall integrity and cellular machinery such that exposed cells gradually die.
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The parts were put together using standard assembly [http://partsregistry.org/Assembly:Standard_assembly]
star: false
creator: IC 2010 Team
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0014
component: BBa_J33204
sequence annotation: BBa_J33204
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K316003_sequence/1

BBa_B0014
type: Component
persistent identity: component2092342
display id: component2092342
version: 1
top level: BBa_K316003
definition: BBa_B0014
access: public

BBa_J33204
type: Component
persistent identity: component2092335
display id: component2092335
version: 1
top level: BBa_K316003
definition: BBa_J33204
access: public

BBa_J33204
type: SequenceAnnotation
persistent identity: annotation2092335
display id: annotation2092335
version: 1
top level: BBa_K316003
location: https://synbiohub.org/public/igem/BBa_K316003/annotation2092335/range2092335/1
component: BBa_J33204

https://synbiohub.org/public/igem/BBa_K316003/annotation2092335/range2092335/1
type: Range
persistent identity: range2092335
display id: range2092335
version: 1
top level: BBa_K316003
direction: direction/reverse
start: 1
end: 958
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2092342
display id: annotation2092342
version: 1
top level: BBa_K316003
location: https://synbiohub.org/public/igem/BBa_K316003/annotation2092342/range2092342/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K316003/annotation2092342/range2092342/1
type: Range
persistent identity: range2092342
display id: range2092342
version: 1
top level: BBa_K316003
direction: direction/reverse
start: 967
end: 1061
orientation: inline

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: iGEM to SBOL conversion
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: non
star: false
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: AraO
sequence annotation: AraO1
sequence annotation: AraPromoter
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
top level: Ara
direction: direction/reverse
start: 1
end: 879
orientation: inline

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
top level: Ara
direction: direction/forward
start: 909
end: 924
orientation: inline

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
top level: Ara
direction: direction/forward
start: 1066
end: 1077
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
top level: Ara
direction: direction/forward
start: 1153
end: 1181
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_J33204
type: ComponentDefinition
persistent identity: BBa J33204
display id: BBa_J33204
version: 1
was generated by: iGEM to SBOL conversion
description: xylE reporter gene with rbs
created: 2006-10-16T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The template DNA was kindly supplied by Dr. Peter Williams of the University of Wales, Bangor. The primer design was based on Genbank sequence M64747 (GI:151718). The sequence reported here was confirmed by sequencing the Biobrick construct.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part includes the xylE gene from the Pseudomonas putida TOL (naphthalene and xylene degradadative plasmid) pWW0. This gene encodes the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This is a useful reporter gene; colonies or broths expressing active XylE, in the presence of oxygen, will rapidly convert catechol, a cheap colourless substrate, to a bright yellow compound with an absorbance maximum around 377 nm. The part includes the native ribosome binding site, so simply needs to be added after a suitable promoter to act as a reporter. I have previously used this gene to generate whole cell biosensors for various heavy metals. Note that, unlike Xgal etc., catechol in solution is prone to spontaneous oxidation resulting in brown melanin-like polymeric products, so is not stable enough to incorporate into plates or growth media; it should be dripped onto colonies (I use 10 mM catechol in water for this) or added to liquid cultures at a final concentration of about 0.5 mM prior to assay. Note also that there is a SacI site at the very start of the part, when the prefix is included, so this can be used as a replacement vector to introduce PCR products with SacI-SpeI ends into pSB1A2 giving them full Biobrick prefixes and suffixes (but don't forget the G base before the SpeI site). This results in shorter non-complementary tails on PCR primers than using a full prefix or suffix. You can test for colonies that have lost xylE using catechol as described above.
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Note that this sequence includes the native ribsomome binding site. Also, when the prefix is included, there is a SacI site at the start, which allows this part to be used as a vector for insertion of PCR products with SacI-SpeI ends into pSB1A2, replacing xylE, giving the full Biobrick prefixes and suffixes (but don't forget the G base before the SpeI site). This results in shorter non-complementary tails on PCR primers than using a full prefix or suffix. You can test for colonies that have lost xylE using catechol as described above.
star: true
creator: Chris French
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_J33204_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1903406
display id: annotation1903406
version: 1
top level: BBa_J33204
location: https://synbiohub.org/public/igem/BBa_J33204/annotation1903406/range1903406/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J33204/annotation1903406/range1903406/1
type: Range
persistent identity: range1903406
display id: range1903406
version: 1
top level: BBa_J33204
direction: direction/forward
start: 14
end: 19
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1903407
display id: annotation1903407
version: 1
top level: BBa_J33204
location: https://synbiohub.org/public/igem/BBa_J33204/annotation1903407/range1903407/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J33204/annotation1903407/range1903407/1
type: Range
persistent identity: range1903407
display id: range1903407
version: 1
top level: BBa_J33204
direction: direction/forward
start: 27
end: 950
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K808033_sequence/1
type: Sequence
persistent identity: BBa K808033 sequence
display id: BBa_K808033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaactactagagaaagaggggacatactagagctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgcca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61100_sequence/1
type: Sequence
persistent identity: BBa J61100 sequence
display id: BBa_J61100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316003_sequence/1
type: Sequence
persistent identity: BBa K316003 sequence
display id: BBa_K316003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccggtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K808000_sequence/1
type: Sequence
persistent identity: BBa K808000 sequence
display id: BBa_K808000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33204_sequence/1
type: Sequence
persistent identity: BBa J33204 sequence
display id: BBa_J33204_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z