BBa_K165010 1 BBa_K165010 ADH1 yeast transcription terminator 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K809301 1 BBa_K809301 RFP + signal peptide of ZIM17 2012-09-22T11:00:00Z 2015-05-08T01:13:26Z http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809201 RFY is linked with signal peptide of ZIM17, thus can be directed into mitochondrial matrix. It is used to test and signal peptide. false false _1066_ 0 12995 9 Not in stock false The part BBa_K809201 and BBa_E1010 should be correctly linked without frame shift false Luohao Xu component2245003 1 BBa_K809201 component2245006 1 BBa_E1010 annotation2245006 1 BBa_E1010 range2245006 1 148 853 annotation2245003 1 BBa_K809201 range2245003 1 1 141 BBa_K809201 1 SPzim17 signal peptide of ZIM17 2012-09-22T11:00:00Z 2015-05-08T01:13:26Z Zim17, a novel zinc finger protein essential for protein import into mitochondria. J Biol Chem. 2004 Nov 26 ;279(48):50243-9. Epub 2004 Sep 21 . http://www.uniprot.org/uniprot/P42844 this part is the sequence of transit signal peptide of ZIM17. It can help protein target to the mitochondrial matrix. false false _1066_ 0 12995 9 It's complicated false it is synthesized based on the complete sequence of signal peptide of ZIM17 false Luohao Xu BBa_K809308 1 BBa_K809308 GAL promoter + RFP_SPzim17 + ADH1 terminator 2012-09-22T11:00:00Z 2015-05-08T01:13:26Z All parts should be correctly linked without frame shift The RFP is linked with signal peptide of Zim17, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be located into mitochondrial matrix. false true _1066_ 0 12995 9 Not in stock false http://partsregistry.org/wiki/index.php?title=Part:BBa_K809601 http://partsregistry.org/wiki/index.php/Part:BBa_K165010 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809301 false Luohao Xu component2251088 1 BBa_K809601 component2251094 1 BBa_K165010 component2251093 1 BBa_K809301 annotation2251093 1 BBa_K809301 range2251093 1 556 1408 annotation2251094 1 BBa_K165010 range2251094 1 1417 1641 annotation2251088 1 BBa_K809601 range2251088 1 1 549 BBa_K809601 1 BBa_K809601 GAL promoter (kozak) without start codon ATG 2012-09-22T11:00:00Z 2015-05-08T01:13:27Z Partsregistry. This is based on BBa_J63006 and is without ATG start codon. It is more convenient to use. false false _1066_ 0 12016 9 It's complicated true nothing false Jianhui Gong annotation2194205 1 promoter range2194205 1 1 549 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_K165010_sequence 1 taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K809601_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggag BBa_K809301_sequence 1 atgattccgaggactagaacactactgcaaagtaaaataccaattaccaggtactttgccagatgttgggcgcctcgcgtacgctacaacgtgtgccgtactctacccgcagcagcgttgcataccaatatcatcgcacattactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K809308_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggagtactagatgattccgaggactagaacactactgcaaagtaaaataccaattaccaggtactttgccagatgttgggcgcctcgcgtacgctacaacgtgtgccgtactctacccgcagcagcgttgcataccaatatcatcgcacattactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K809201_sequence 1 atgattccgaggactagaacactactgcaaagtaaaataccaattaccaggtactttgccagatgttgggcgcctcgcgtacgctacaacgtgtgccgtactctacccgcagcagcgttgcataccaatatcatcgcacat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z