BBa_K165010
1
BBa_K165010
ADH1 yeast transcription terminator
2008-10-25T11:00:00Z
2015-05-08T01:10:55Z
-
-
false
false
_267_
0
2512
58
It's complicated
false
-
false
John Szymanski
BBa_K809202
1
SPfer
signal peptide of FER
2012-09-22T11:00:00Z
2015-05-08T01:13:26Z
Chloroplast transit peptides: structure, function and evolution, Barry D Bruce, Trends in Cell Biology - 1 October 2000 (Vol. 10, Issue 10, pp. 440-447)
http://www.uniprot.org/blast/?about=P07839[1-32]
This is the signal peptide of ferredoxi from chroloplast, which can help protein target to matrix of chroloplast
false
true
_1066_
0
12995
9
It's complicated
false
it is synthesized without any modification for the original sequence
false
Luohao Xu
BBa_K809601
1
BBa_K809601
GAL promoter (kozak) without start codon ATG
2012-09-22T11:00:00Z
2015-05-08T01:13:27Z
Partsregistry.
This is based on BBa_J63006 and is without ATG start codon. It is more convenient to use.
false
false
_1066_
0
12016
9
It's complicated
true
nothing
false
Jianhui Gong
annotation2194205
1
promoter
range2194205
1
1
549
BBa_K809302
1
RFY_SPfer
RFP + signal peptide of FER
2012-09-22T11:00:00Z
2015-05-08T01:13:26Z
http://partsregistry.org/wiki/index.php?title=Part:BBa_E1010
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809202
RFY is linked with signal peptide of ferredoxi, thus can be directed into matrix of chloroplast. It is used to test and signal peptide.
false
true
_1066_
0
12995
9
Not in stock
false
The part BBa_K809202 and BBa_E1010 should be correctly linked without frame shift
false
Luohao Xu
component2245011
1
BBa_K809202
component2245014
1
BBa_E1010
annotation2245011
1
BBa_K809202
range2245011
1
1
96
annotation2245014
1
BBa_E1010
range2245014
1
103
808
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation1014044
1
mrfp1
range1014044
1
1
675
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
BBa_K809309
1
BBa_K809309
GAL promoter + RFP_SPfer + ADH1 terminator
2012-09-22T11:00:00Z
2015-05-08T01:13:26Z
All parts should be correctly linked without frame shift
The RFP is linked with signal peptide of ferredoxi, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be located into matrix of chloroplast.
false
false
_1066_
0
12995
9
Not in stock
false
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809601
http://partsregistry.org/wiki/index.php/Part:BBa_K165010
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809301
false
Luohao Xu
component2251101
1
BBa_K809302
component2251102
1
BBa_K165010
component2251096
1
BBa_K809601
annotation2251096
1
BBa_K809601
range2251096
1
1
549
annotation2251101
1
BBa_K809302
range2251101
1
556
1363
annotation2251102
1
BBa_K165010
range2251102
1
1372
1596
BBa_K165010_sequence
1
taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
BBa_K809202_sequence
1
atggccatggctatgcgctccaccttcgccgcccgcgttggcgctaagcccgctgtccgcggtgctcgccccgccagccgcatgagctgcatggcc
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_K809309_sequence
1
ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggagtactagatggccatggctatgcgctccaccttcgccgcccgcgttggcgctaagcccgctgtccgcggtgctcgccccgccagccgcatgagctgcatggcctactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc
BBa_K809601_sequence
1
ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggag
BBa_K809302_sequence
1
atggccatggctatgcgctccaccttcgccgcccgcgttggcgctaagcccgctgtccgcggtgctcgccccgccagccgcatgagctgcatggcctactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z