BBa_K165010 1 BBa_K165010 ADH1 yeast transcription terminator 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K809203 1 SPtim21 signal peptide of TIM21 2012-09-22T11:00:00Z 2015-05-08T01:13:26Z Chacinska, A., M. Lind, et al. (2005). "Mitochondrial Presequence Translocase: Switching between TOM Tethering and Motor Recruitment Involves Tim21 and Tim17." Cell 120(6): 817-829. This is the signal peptide of TIM21, which helps protein target to the inner membrane of mitochondrion false true _1066_ 0 12995 9 It's complicated false It is synthesized entirely from the original sequence false Luohao Xu BBa_K809601 1 BBa_K809601 GAL promoter (kozak) without start codon ATG 2012-09-22T11:00:00Z 2015-05-08T01:13:27Z Partsregistry. This is based on BBa_J63006 and is without ATG start codon. It is more convenient to use. false false _1066_ 0 12016 9 It's complicated true nothing false Jianhui Gong annotation2194205 1 promoter range2194205 1 1 549 BBa_E1030 1 cohmYFP cohmYFP (July 2004) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z A.victoria and computation (c/o DNA 2.0 and others) cohmYFP: Codon Optimized, Homology Minimized Yellow Fluorescent Protein. Excitiation peak: ### nm Emission peak: ### nm false false _11_1_ 0 52 7 Not in stock false false Drew Endy annotation2214016 1 Help:Barcodes range2214016 1 6 30 BBa_K809315 1 YFP_SPtim2 YFP + signal peptide of Tim21 2012-09-24T11:00:00Z 2015-05-08T01:13:26Z http://partsregistry.org/wiki/index.php?title=Part:BBa_E1030 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809203 YFP is linked with signal peptide of Tim21, thus can be directed to mitochondrial inner membrane. It is used to test and signal peptide. false true _1066_ 0 12995 9 Not in stock false Both parts should be linked correctly without frame shift and nonsense mutation false Luohao Xu component2245038 1 BBa_E1030 component2245036 1 BBa_K809203 annotation2245036 1 BBa_K809203 range2245036 1 1 96 annotation2245038 1 BBa_E1030 range2245038 1 105 134 BBa_K809321 1 BBa_K809321 GAL promoter + YFP_SPtim21 + ADH1 terminator 2012-09-25T11:00:00Z 2015-05-08T01:13:26Z http://partsregistry.org/wiki/index.php?title=Part:BBa_K809601 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809315 http://partsregistry.org/wiki/index.php?title=Part:BBa_K165010 The YFP is linked with signal peptide of Tim21, GAL promoter and ADH1 terminator. When expressed in cytoplasm, it will be located into mitochondrial inner membrane. false false _1066_ 0 12995 9 Not in stock false All parts should be linked correctly without frameshift mutation false Luohao Xu component2251206 1 BBa_K809601 component2251210 1 BBa_K809315 component2251211 1 BBa_K165010 annotation2251210 1 BBa_K809315 range2251210 1 556 689 annotation2251211 1 BBa_K165010 range2251211 1 698 922 annotation2251206 1 BBa_K809601 range2251206 1 1 549 BBa_K165010_sequence 1 taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K809321_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggagtactagatggccatggctatgcgctccaccttcgccgcccgcgttggcgctaagcccgctgtccgcggtgctcgccccgccagccgcatgagctgcatggcctactagagtaatacactgatagtgctagtgtagatcactactagagtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K809601_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggag BBa_E1030_sequence 1 taatacactgatagtgctagtgtagatcac BBa_K809315_sequence 1 atggccatggctatgcgctccaccttcgccgcccgcgttggcgctaagcccgctgtccgcggtgctcgccccgccagccgcatgagctgcatggcctactagagtaatacactgatagtgctagtgtagatcac BBa_K809203_sequence 1 atggccatggctatgcgctccaccttcgccgcccgcgttggcgctaagcccgctgtccgcggtgctcgccccgccagccgcatgagctgcatggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z