BBa_K165010 1 BBa_K165010 ADH1 yeast transcription terminator 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - - false false _267_ 0 2512 58 It's complicated false - false John Szymanski BBa_K809208 1 Toc34 Toc34 protein 2012-09-25T11:00:00Z 2015-05-08T01:13:26Z http://merolae.biol.s.u-tokyo.ac.jp/db/cds_view.cgi?id=665 Toc34 is a GTPase protein that functions as signal peptides receptor, N-terminally anchored at chloroplast outer membrane. false false _1066_ 0 12995 9 Not in stock false It was synthesized according to the sequence exactly false Luohao Xu BBa_K809317 1 YFP_SPtom7 YFP + signal peptide of Tom70 2012-09-24T11:00:00Z 2015-05-08T01:13:26Z http://partsregistry.org/wiki/index.php?title=Part:BBa_E1030 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809205 YFP is linked with signal peptide of Tom70, thus can be directed to mitochondrial outer membrane. It is used to test and signal peptide. false false _1066_ 0 12995 9 Not in stock false Both parts should be linked correctly without frame shift and nonsense mutation false Luohao Xu component2245065 1 BBa_K809205 component2245067 1 BBa_E1030 annotation2245065 1 BBa_K809205 range2245065 1 1 90 annotation2245067 1 BBa_E1030 range2245067 1 99 128 BBa_K809330 1 BBa_K809330 GAL promoter + YFP + SPtom70 + Toc34+ ADH1 terminator 2012-09-25T11:00:00Z 2015-05-08T01:13:26Z http://partsregistry.org/wiki/index.php?title=Part:BBa_K809601 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809317 http://partsregistry.org/wiki/index.php?title=Part:BBa_K809208 http://partsregistry.org/wiki/index.php?title=Part:BBa_K165010 BioBricks of Toc34_SPtom70 (K809324) is linked with GAL promoter and ADH1 terminator. When expressed in cytoplasm, The Toc34 will be directed on mitochondrial outer membrane, and the YFP can function as a reporter. false false _1066_ 0 12995 9 Not in stock false All parts should be linked correctly without nonsense mutation and frameshift mutation false Luohao Xu component2259713 1 BBa_K809317 component2259714 1 BBa_K809208 component2259709 1 BBa_K809601 component2259715 1 BBa_K165010 annotation2259715 1 BBa_K165010 range2259715 1 2165 2389 annotation2259713 1 BBa_K809317 range2259713 1 556 683 annotation2259714 1 BBa_K809208 range2259714 1 690 2156 annotation2259709 1 BBa_K809601 range2259709 1 1 549 BBa_K809205 1 SPtom70 signal peptide of Tom70 2012-09-22T11:00:00Z 2015-05-08T01:13:26Z Rapaport, D. (2003). "Finding the right organelle." EMBO Rep 4(10): 948-952. This is the signal peptide of Tom70, which can direct N-terminally anchored protein to mitochondrial outer membrane false false _1066_ 0 12995 9 It's complicated false It is synthesized according to the sequence found in datebase false Luohao Xu BBa_E1030 1 cohmYFP cohmYFP (July 2004) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z A.victoria and computation (c/o DNA 2.0 and others) cohmYFP: Codon Optimized, Homology Minimized Yellow Fluorescent Protein. Excitiation peak: ### nm Emission peak: ### nm false false _11_1_ 0 52 7 Not in stock false false Drew Endy annotation2214016 1 Help:Barcodes range2214016 1 6 30 BBa_K809601 1 BBa_K809601 GAL promoter (kozak) without start codon ATG 2012-09-22T11:00:00Z 2015-05-08T01:13:27Z Partsregistry. This is based on BBa_J63006 and is without ATG start codon. It is more convenient to use. false false _1066_ 0 12016 9 It's complicated true nothing false Jianhui Gong annotation2194205 1 promoter range2194205 1 1 549 BBa_K809208_sequence 1 atggactcggagacgcctaccaagaatgaagcggaacagcgtgcttcaccagatcgaactgggcaagttacccagcggcctgtttcgctgttcaggaaaaacggaacctttgcctatcctctagaagactctgacgtttacgaagagcaagtgcttgcacttgaggacgtacccgagccgactgctcaagcgattctgcgtctcgcactggcagcggaacgacaaggagtgccacggctgcgatttctggtcgtgggagcacctggtgttggcaagtcgtccctgatcaacacgctcctgaacgagaacctgtgctctgtgtctgcgtgggagcggggaacgaaaaacgcgcaagtatgtgctcgacaagtcgactccgtggtgatcgagttcattgataccccagggatagcaccttgtagacgcagcggcctagaagctagtcgacggcaggtccaacggcttcgaaagctcctcgacgctcggggtgcggatgagcatccttatctgcgttccttccacgctattctctacgtcatgcgtctcgatgacacccgccctgatctagttgattaccacaattggaaagttctgatggaatttttcggggcagaagtgcttcgccacatgatggtagtctttacccatggtcaatcattgccgcctgactcgctgagctaccccgaatacgtccgcggtcgtcgtgactacgtgtacttacttattgaacgtctcactgggccgctaaaagccgtccggtttcccgtctttgtcgcagagaattccagcaaatgcccggtgatagaggaaaccggcgagcgaaagctaccagacgatacgccatggattacgcagctttacgatgggattcgtcgattcgttttcccgttggaagcagatttattcggcatggaaacgggacctgaggatacccaggtgctggtctacatctatgacaagcgccgctctcgagcagatgagcggtatcctcgttcaccgcttcaagcgctgataaggaacccgtggtttctacgagctgtgcagttttgtggcgcaatgctgttgcgcagttacatgattcggcaagagcacgagcgcttcaacccgaaaccgcaccatccagaaccgatcatccgagatgcgaacaatgctttcgcggtagcatcgcgcagggccaatgatcaccgcctcttcacaccaagcatcgatgaagacgaggaatccttctttcggatgaacggaaacgatgattttgtcagtcgacttcgaaatcgccctggcgaggtcgatcttgacgagctcgatgtcgagcgaatcgagagccccgacggacgttttgtggaatactggtacaccgaaaaggataatccggaaatggtgcggatgtatcggctcgaaatacgtgacgatcccaacgaggagcgcgaaagccgccaggatcggataccgatctcaaaggaccagcac BBa_K809317_sequence 1 atgaagagcttcattacaaggaacaagacagccattttggcaaccgttgctgctacaggtactgccatcggtgcctactattattacaactactagagtaatacactgatagtgctagtgtagatcac BBa_K165010_sequence 1 taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K809601_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggag BBa_K809205_sequence 1 atgaagagcttcattacaaggaacaagacagccattttggcaaccgttgctgctacaggtactgccatcggtgcctactattattacaac BBa_E1030_sequence 1 taatacactgatagtgctagtgtagatcac BBa_K809330_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccacggagtactagatgaagagcttcattacaaggaacaagacagccattttggcaaccgttgctgctacaggtactgccatcggtgcctactattattacaactactagagtaatacactgatagtgctagtgtagatcactactagatggactcggagacgcctaccaagaatgaagcggaacagcgtgcttcaccagatcgaactgggcaagttacccagcggcctgtttcgctgttcaggaaaaacggaacctttgcctatcctctagaagactctgacgtttacgaagagcaagtgcttgcacttgaggacgtacccgagccgactgctcaagcgattctgcgtctcgcactggcagcggaacgacaaggagtgccacggctgcgatttctggtcgtgggagcacctggtgttggcaagtcgtccctgatcaacacgctcctgaacgagaacctgtgctctgtgtctgcgtgggagcggggaacgaaaaacgcgcaagtatgtgctcgacaagtcgactccgtggtgatcgagttcattgataccccagggatagcaccttgtagacgcagcggcctagaagctagtcgacggcaggtccaacggcttcgaaagctcctcgacgctcggggtgcggatgagcatccttatctgcgttccttccacgctattctctacgtcatgcgtctcgatgacacccgccctgatctagttgattaccacaattggaaagttctgatggaatttttcggggcagaagtgcttcgccacatgatggtagtctttacccatggtcaatcattgccgcctgactcgctgagctaccccgaatacgtccgcggtcgtcgtgactacgtgtacttacttattgaacgtctcactgggccgctaaaagccgtccggtttcccgtctttgtcgcagagaattccagcaaatgcccggtgatagaggaaaccggcgagcgaaagctaccagacgatacgccatggattacgcagctttacgatgggattcgtcgattcgttttcccgttggaagcagatttattcggcatggaaacgggacctgaggatacccaggtgctggtctacatctatgacaagcgccgctctcgagcagatgagcggtatcctcgttcaccgcttcaagcgctgataaggaacccgtggtttctacgagctgtgcagttttgtggcgcaatgctgttgcgcagttacatgattcggcaagagcacgagcgcttcaacccgaaaccgcaccatccagaaccgatcatccgagatgcgaacaatgctttcgcggtagcatcgcgcagggccaatgatcaccgcctcttcacaccaagcatcgatgaagacgaggaatccttctttcggatgaacggaaacgatgattttgtcagtcgacttcgaaatcgccctggcgaggtcgatcttgacgagctcgatgtcgagcgaatcgagagccccgacggacgttttgtggaatactggtacaccgaaaaggataatccggaaatggtgcggatgtatcggctcgaaatacgtgacgatcccaacgaggagcgcgaaagccgccaggatcggataccgatctcaaaggaccagcactactagagtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z