BBa_K809005
1
COX3 termi
Terminator of Yeast mitochondrial gene COX3
2012-09-22T11:00:00Z
2015-05-08T01:13:26Z
This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region.
http://www.yeastgenome.org/
This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
false
false
_1066_
0
12018
9
Not in stock
false
We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
false
Xiaopeng Xu
annotation2194193
1
stem_loop
range2194193
1
16
34
BBa_K809009
1
OLI1 RBS
RBS sequence of yeast mitochondrial gene OLI1
2012-09-23T11:00:00Z
2015-05-08T01:13:26Z
This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
This is the RBS region for OLI1 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
false
false
_1066_
0
12018
9
Not in stock
false
The sequence between the last ATG codon and CDS on the 3'UTR DNA sequence is believed to contain RBS. With some modifications, we made those sequences BioBricks.
false
Xiaopeng Xu
BBa_K809402
1
P+ROP
Promoter+ROP in mitochondria
2012-09-23T11:00:00Z
2015-05-08T01:13:27Z
1. Brekasis, D. and M. S. Paget (2003). "A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2)." EMBO J 22(18): 4856-4865.
2. Deshpande, A. P. and S. S. Patel (2012). "Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase." Biochim Biophys Acta.
This is regulatory sequence which can be repressed by binding of REX on ROP site.
false
false
_1066_
0
12018
9
Not in stock
false
With different place of ROP and promoter, the effect of REX binding on ROP might differs.
false
Xiaopeng Xu
BBa_K809001
1
mtGFP
Mitochondrial GFP
2012-09-19T11:00:00Z
2015-05-08T01:13:26Z
..
Mitochondrial GFP
false
false
_1066_
0
11943
9
It's complicated
true
..
false
Qiwu XU
annotation2188517
1
mt gfp
range2188517
1
1
714
BBa_K809503
1
BBa_K809503
P+ROP+OLI1 RBS+mtGFP+COX3 terminator
2012-09-23T11:00:00Z
2015-05-08T01:13:27Z
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809402
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809001
http://partsregistry.org/wiki/index.php?title=Part:BBa_K809005
This biobrick is to produce GFP with the control of REX binding on ROP site.
false
false
_1066_
0
12018
9
Not in stock
false
All parts should be correctly linked without frame shift and Restriction Enzyme cutting site.
false
Xiaopeng Xu
component2196295
1
BBa_K809402
component2196300
1
BBa_K809005
component2196298
1
BBa_K809001
component2196296
1
BBa_K809009
annotation2196298
1
BBa_K809001
range2196298
1
170
886
annotation2196296
1
BBa_K809009
range2196296
1
50
163
annotation2196300
1
BBa_K809005
range2196300
1
895
943
annotation2196295
1
BBa_K809402
range2196295
1
1
43
BBa_K809009_sequence
1
atgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatt
BBa_K809402_sequence
1
atataagtaatttgcttgtgaatgtgaacgcgttcacaagcgt
BBa_K809001_sequence
1
atgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataa
BBa_K809005_sequence
1
aaaactcctaacggggttcccgcgaagcgggaactaataataatataat
BBa_K809503_sequence
1
atataagtaatttgcttgtgaatgtgaacgcgttcacaagcgttactagatgttaattatatataatatatttatatataattatatatatatatataaataataataaatatatatataatataaaaataagaatagattaaatatttaataaataaatatttactagatgtcaaaaggtgaagaattattcactggagtagtacctatcttagtagaattagatggtgatgtaaatggtcataaattctcagtatcaggtgaaggtgaaggtgatgctacatatggtaaattaacattaaaattcatctgtacaacaggtaaattacctgtaccttgacctacattagtaacaacattctcatatggagtacaatgtttctcaagatatcctgatcatatgaaacaacatgatttcttcaaatcagcaatgcctgaaggttacgtacaagaaagaacaatcttcttcaaagatgatggtaattataaaacaagagctgaagtaaaattcgaaggtgatacattagtaaatagaatcgagttaaaaggtatcgatttcaaagaagatggtaatatcttaggtcataaattagaatataattataattcacataatgtatatatcatggctgataaacaaaaaaatggtatcaaagtaaatttcaaaatcagacataatatcgaagacggttcagtacaattagcagatcattatcaacaaaatacacctatcggtgatggtcctgtattattacctgataatcattacttaagtacacaatcagctttatcaaaagatcctaatgaaaaaagagatcatatggtattattagaatttgtaacagctgctggtatcacacatggtatggatgaattatataaataatactagagaaaactcctaacggggttcccgcgaagcgggaactaataataatataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z