LOCUS BBa_K809711 3587 bp DNA linear 22-Dec-2024 DEFINITION GAL promoter + kozak + ZIM17+ T7 RNAP + ADH1 terminator ACCESSION BBa_K809711 VERSION BBa_K809711.1 FEATURES Location/Qualifiers promoter 1..549 /label=promoter promoter 1..549 /label=BBa_K809601 misc_feature 556..696 /label=BBa_K809201 CDS 703..3354 /label=cds CDS 703..3354 /label=BBa_K809602 terminator 3363..3587 /label=BBa_K165010 ORIGIN 1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg 61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg 121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc 181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag 241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa 301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa 361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac 421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa 481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc 541 accacggagt actagatgat tccgaggact agaacactac tgcaaagtaa aataccaatt 601 accaggtact ttgccagatg ttgggcgcct cgcgtacgct acaacgtgtg ccgtactcta 661 cccgcagcag cgttgcatac caatatcatc gcacattact agatgaacac gattaacatc 721 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac 781 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg 841 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat 901 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac 961 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg 1021 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta 1081 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag 1141 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt 1201 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc 1261 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag 1321 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg 1381 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc 1441 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg 1501 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat 1561 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg 1621 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc 1681 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat 1741 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac 1801 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc 1861 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat 1921 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt 1981 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg 2041 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac 2101 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac 2161 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat 2221 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg 2281 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc 2341 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc 2401 gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca 2461 atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct 2521 gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact 2581 cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc 2641 cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg 2701 ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc 2761 gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg 2821 gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat 2881 tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc 2941 ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat 3001 agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa 3061 gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct 3121 tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa 3181 gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac 3241 gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct 3301 aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaatactag 3361 agtaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa 3421 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga 3481 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg 3541 accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccc // LOCUS BBa_K809710 3587 bp DNA linear 22-Dec-2024 DEFINITION GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator ACCESSION BBa_K809710 VERSION BBa_K809710.1 FEATURES Location/Qualifiers promoter 1..549 /label=promoter promoter 1..549 /label=BBa_K809601 misc_feature 556..696 /label=BBa_K809201 CDS 703..3354 /label=cds CDS 703..3354 /label=BBa_K809602 terminator 3363..3587 /label=BBa_K165010 ORIGIN 1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg 61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg 121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc 181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag 241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa 301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa 361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac 421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa 481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc 541 accacggagt actagatgat tccgaggact agaacactac tgcaaagtaa aataccaatt 601 accaggtact ttgccagatg ttgggcgcct cgcgtacgct acaacgtgtg ccgtactcta 661 cccgcagcag cgttgcatac caatatcatc gcacattact agatgaacac gattaacatc 721 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac 781 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg 841 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat 901 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac 961 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg 1021 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta 1081 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag 1141 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt 1201 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc 1261 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag 1321 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg 1381 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc 1441 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg 1501 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat 1561 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg 1621 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc 1681 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat 1741 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac 1801 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc 1861 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat 1921 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt 1981 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg 2041 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac 2101 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac 2161 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat 2221 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg 2281 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc 2341 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc 2401 gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca 2461 atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct 2521 gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact 2581 cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc 2641 cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg 2701 ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc 2761 gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg 2821 gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat 2881 tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc 2941 ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat 3001 agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa 3061 gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct 3121 tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa 3181 gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac 3241 gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct 3301 aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaatactag 3361 agtaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa 3421 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga 3481 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg 3541 accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccc //