LOCUS       BBa_K809713             1008 bp    DNA     linear       26-Dec-2024
DEFINITION  T7 promoter + kozak + GFP + ADH1 terminator
ACCESSION   BBa_K809713
VERSION     BBa_K809713.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    44..46
                     /label=start codon
     RBS             32..49
                     /label=BBa_J63003
     CDS             56..775
                     /label=GFP protein
     CDS             56..775
                     /label=BBa_E0040
     terminator      784..1008
                     /label=BBa_K165010
ORIGIN
        1 taatacgact cactataggg agatactaga gcccgccgcc accatggagt actagatgcg
       61 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      121 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      181 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      241 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
      301 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      361 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      421 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      481 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
      541 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
      601 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      661 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
      721 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
      781 gagtaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
      841 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
      901 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt
      961 gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa tcgctccc
//