LOCUS       BBa_K809720             2924 bp    DNA     linear       29-Nov-2024
DEFINITION  DLD3 promoter + kozak + yeast GFP+ ADH1 terminator
ACCESSION   BBa_K809720
VERSION     BBa_K809720.1  
FEATURES             Location/Qualifiers
     promoter        1..1939
                     /label=BBa_K809606
     misc_feature    1960..1962
                     /label=start codon
     RBS             1948..1965
                     /label=BBa_J63003
     CDS             1972..2691
                     /label=GFP protein
     CDS             1972..2691
                     /label=BBa_E0040
     terminator      2700..2924
                     /label=BBa_K165010
ORIGIN
        1 tggtatagtc ccgtacacac tctatttgaa taaactgtag gttctgctga gcaacatatt
       61 gatataaaca gtaaaaagaa gtgggttcct tgttttcctg caattataac taaccgtgct
      121 tactaccaaa aaaaaagtgc gtataaatat tcactattta caatgttgaa ggttccagat
      181 ggatacagaa taggaatcct aaatataaac actatattta tttatttttt tcgaataaaa
      241 gtcatgttag caatatttat atagttgatt ttcctgcatt tattatataa ttggcggcat
      301 agtaacatca aaaaaaaaaa aaaaaacatt caaattgcac tacttatagc agtagcatct
      361 ataaaagttt tatgatataa acctttacag ttatctcaaa tggttttggg attgtccatc
      421 aaatcaagcg tgattcatct atacgttatt attctcctac aaccccctta gtttggagag
      481 cgcttagaga aataaagcat cgatgttttc ttaccttcac taaaatcaaa ataaaaaagc
      541 ttttctatag ttcacgaatt acgcaattta ttctatcatc cttaaagttg atcaattgcg
      601 attttaacac acattttcaa atgtcatttt gcgcttgtat aataggtgcc atctggacac
      661 tttataaaaa ataaaggaaa agtaaaatat aatgctgaaa atatagttgc cttaaaatgc
      721 cattgatatg attttttttt aaccttaaat ctattggcct ataatattcg cggcaaaatg
      781 agcatttgaa aatcagtaat agaatgctat caaacttcgt tcatgacctt tttagcatca
      841 taagtgttga atcgaatcta tcagttcagt aattaaaaat actatttttc tgtcagcaca
      901 gaacgtaacg acttttcctt atatccacta ttgatttctc tttaaagtgc tatgtaaagt
      961 acctaaaaaa cttgttatag aagtctcatt cgaaagcatg tgggctcaaa aattgtgaat
     1021 aagatacata gaaggagccg ctgatcgaca cataatagta gagttgaacc tagttggttc
     1081 aaccattatc atagttatac gaataaagcc acaatataaa tggccaaccg ctgttgtcaa
     1141 acaagactat aggttcattt taacctggcg tgcagtacgc tggtcttgct aacgcctcta
     1201 caatacgtac agtgcaggtt ttgacgaatg aaagtggtta gaagattaat gtgatagaaa
     1261 caagaatttt ctcgcaaaga tctgtatgaa aggaaacgtt tggtaagaac tctgcgatcc
     1321 aaattctact gaatgaatag aaggcttcta cagaacaagt tgtatcgaaa tgattgttgg
     1381 cgactactat atttctatag gcatgaaagt tactcataaa caggaaagac gcattttagt
     1441 agcttgacct ggtcagatta atcagcttcc aacgttactt ccctttcgca agaatctacc
     1501 caaaatgtct cgagcatctt gataattaca gtatcgttcg tcccgacttg gcatttgttt
     1561 aaatttctaa gatgcttcct ataggaacat aattgtcaag aaagcacaac aaattgtctg
     1621 caatgtcaac aggagtggcg cattttatgt tttttcattt tttttttttt gtgcgtgatc
     1681 attaagcggg atattgtcca cagtcatcta aaagaatgac catttcgacg acttagttcg
     1741 gaaaatattt ccagcggatg acaccacttg ccacagttgg tgaccgccaa atctaagtca
     1801 cgcgcggaaa ctgaaaggtt gtgagtatat aagtgatcac tcgcttatat aactgacgag
     1861 gcagaacagg gtgccaaaat gctcctcaat attttattca tttgagattc aaggcttaaa
     1921 gacagcatat ataagaattt actagagccc gccgccacca tggagtacta gatgcgtaaa
     1981 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     2041 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     2101 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     2161 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     2221 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     2281 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     2341 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     2401 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     2461 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     2521 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     2581 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     2641 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagt
     2701 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     2761 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     2821 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     2881 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccc
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