LOCUS BBa_K809722 2229 bp DNA linear 26-Nov-2024 DEFINITION DLD3 promoter + kozak + yeast GFP + hsl1 tag +ADH1 terminator ACCESSION BBa_K809722 VERSION BBa_K809722.1 FEATURES Location/Qualifiers misc_feature 1..618 /label=BBa_K105015 misc_feature 639..641 /label=start codon RBS 627..644 /label=BBa_J63003 CDS 651..1370 /label=GFP protein CDS 651..1370 /label=BBa_E0040 misc_feature 1379..1996 /label=BBa_K105015 terminator 2005..2229 /label=BBa_K165010 ORIGIN 1 caaaactcgg cttcaaagag atccttatac tcattacagt caatttcaaa acgttccttg 61 aacctgaatg atttattagt ctttgacgat cctctcccta gtaagaaacc agcatccgaa 121 aatgtgaata aatcagaacc ccattcgttg gaatcagact ctgattttga gatcttatgc 181 gatcagatct tatttggaaa cgccttagat aggatactcg aagaggaaga ggacaatgaa 241 aaagaacgcg acacccaaag acagaggcaa aacgatacaa aatcttcggc agacactttt 301 acgatctctg gggtgtctac aaataaggaa aatgagggcc cggagtatcc aaccaaaatt 361 gagaaaaatc aattcaatat gtcatataaa ccatctgaga atatgtcagg gctctcttca 421 tttcctatct ttgaaaaaga aaacacgtta agctcaagtt atttggaaga acagaagcca 481 aagagagcgg ccctttcaga tatcacgaac tcattcaata aaatgaataa acaggaaggt 541 atgaggatag aaaaaaagat tcaaagagaa caacttcaaa aaaaaaatga ccgcccgtca 601 ccactcaaac ctattcagta ctagagcccg ccgccaccat ggagtactag atgcgtaaag 661 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 721 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 781 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 841 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 901 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 961 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 1021 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 1081 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 1141 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 1201 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 1261 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 1321 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagca 1381 aaactcggct tcaaagagat ccttatactc attacagtca atttcaaaac gttccttgaa 1441 cctgaatgat ttattagtct ttgacgatcc tctccctagt aagaaaccag catccgaaaa 1501 tgtgaataaa tcagaacccc attcgttgga atcagactct gattttgaga tcttatgcga 1561 tcagatctta tttggaaacg ccttagatag gatactcgaa gaggaagagg acaatgaaaa 1621 agaacgcgac acccaaagac agaggcaaaa cgatacaaaa tcttcggcag acacttttac 1681 gatctctggg gtgtctacaa ataaggaaaa tgagggcccg gagtatccaa ccaaaattga 1741 gaaaaatcaa ttcaatatgt catataaacc atctgagaat atgtcagggc tctcttcatt 1801 tcctatcttt gaaaaagaaa acacgttaag ctcaagttat ttggaagaac agaagccaaa 1861 gagagcggcc ctttcagata tcacgaactc attcaataaa atgaataaac aggaaggtat 1921 gaggatagaa aaaaagattc aaagagaaca acttcaaaaa aaaaatgacc gcccgtcacc 1981 actcaaacct attcagtact agagtaataa gcgaatttct tatgatttat gatttttatt 2041 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta 2101 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta 2161 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa atgcctgcaa 2221 atcgctccc //