BBa_K810000
1
BBa_K810000
Lambda phage beta CDS with T5 promoter
2012-09-27T11:00:00Z
2015-05-08T01:13:27Z
The promoter region was amplified from the plasmid pBAV1K-T5-gfp (Bryksin and Matsumura, 2010). The coding sequence was amplified from E. coli strain SIMD50 as described in Datta, et al., 2006. This coding sequence is originally from the bacteriophage lambda.
This part features the coding sequence for the recombinase of the bacteriophage lambda, called beta. It has been fused with a promoter designed by Bryksin and Matsumura (2010) that has been shown to promote transcription in both Gram-positive and Gram-negative organisms. It is flanked by XbaI and SpeI and features a KpnI restriction enzyme site between the promoter and coding sequence. It was designed to go in plasmid pBAV1K (Bryksin and Matsumura) which replicates in both Gram-positive and Gram-negative bacteria.
false
false
_1067_
0
12597
9
It's complicated
false
The CDS for beta is downstream of the T5-lac promoter from plasmid pBAV1K-T5 described by Bryksin and Matsumura, 2010. The construct was designed to be cloned into pBAV1K to create a construct that could be used across species.
false
Spencer R Katz
annotation2203769
1
T5 promoter
range2203769
1
1
57
annotation2203773
1
LacO
range2203773
1
39
57
annotation2203772
1
LacO
range2203772
1
7
25
annotation2203767
1
bet CDS
range2203767
1
83
868
annotation2203774
1
KpnI restriction enzyme site
range2203774
1
77
82
annotation2203771
1
-10 signal
range2203771
1
24
31
annotation2203768
1
RBS
range2203768
1
65
76
annotation2203770
1
-35 signal
range2203770
1
1
7
BBa_K810000_sequence
1
tttgctttgtgagcggataacaattataatagattcaattgtgagcggataacaattactagagaaagaggagaaaggtaccatgagtactgcactcgcaacgctggctgggaagctggctgaacgtgtcggcatggattctgtcgacccacaggaactgatcaccactcttcgccagacggcatttaaaggtgatgccagcgatgcgcagttcatcgcattactgatcgttgccaaccagtacggccttaatccgtggacgaaagaaatttacgcctttcctgataagcagaatggcatcgttccggtggtgggcgttgatggctggtcccgcatcatcaatgaaaaccagcagtttgatggcatggactttgagcaggacaatgaatcctgtacatgccggatttaccgcaaggaccgtaatcatccgatctgcgttaccgaatggatggatgaatgccgccgcgaaccattcaaaactcgcgaaggcagagaaatcacggggccgtggcagtcgcatcccaaacggatgttacgtcataaagccatgattcagtgtgcccgtctggccttcggatttgctggtatctatgacaaggatgaagccgagcgcattgtcgaaaatactgcatacactgcagaacgtcagccggaacgcgacatcactccggttaacgatgaaaccatgcaggagattaacactctgctgatcgccctggataaaacatgggatgacgacttattgccgctctgttcccagatatttcgccgcgacattcgtgcatcgtcagaactgacacaggccgaagcagtaaaagctcttggattcctgaaacagaaagccgcagagcagaaggtggcagcatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z