LOCUS BBa_K812000 4113 bp DNA linear 23-Dec-2024 DEFINITION Biobricked pCS2+ plasmid for frogs and chicken ACCESSION BBa_K812000 VERSION BBa_K812000.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=Biobrick suffix primer_bind 26..42 /label=T7 prom (rev) misc_feature 47..242 /label=SV40 Polyadenylation site misc_feature 243..275 /label=Downstream polylinker (from pVZ1) primer_bind 293..312 /label=T3_promoter primer_bind 329..347 /label=M13 primer misc_feature 679..1346 /label=ColE1 rep origin CDS 1494..2344 /label=Ampicillin resistance Bla gene promoter 2396..2424 /label=AmpR promoter misc_feature 2503..2809 /label=E. coli f1replication origin promoter 3012..3998 /label=CMV eukaryotic promoter primer_bind 3916..3957 /label=CMV_fwd_primer primer_bind 4033..4051 /label=Sp6 primer misc_feature 4093..4113 /label=Biobrick prefix ORIGIN 1 tactagtagc ggccgctgca ggaactatag tgagtcgtat tacgtagatc cagacatgat 61 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 121 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 181 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 241 ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag 301 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 1021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 1081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 1141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 1201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 1261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 1321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 1381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 2041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 2101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 2161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 2221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 2281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 2341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 2401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2461 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 2521 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 2581 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 2641 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 2701 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 2761 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 2821 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 2881 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 2941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 3001 tattacgcca gtcgaccata gccaattcaa tatggcgtat atggactcat gccaattcaa 3061 tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt gccaattcaa 3121 tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat gccaattcaa 3181 tatggcggac ttggcactgt gccaactggg gaggggtcta cttggcacgg tgccaagttt 3241 gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg tgccaataat 3301 ggcggccata ttggctatat gccaggatca atatataggc aatatccaat atggccctat 3361 gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca tatagtattc 3421 catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc ccatatacca 3481 tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa tggtctctat 3541 atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg gcgcctcccc 3601 cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac gtcaatagga 3661 ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt tctcacgccc 3721 cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat ctattaatag 3781 taacttggca agtacattac tattggaagc acgccagggt acattggcag tactcccatt 3841 gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat tgacgtcaat 3901 ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt aggcgtgcct 3961 aatgggaggt ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc 4021 ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat 4081 cccatcgatt cgaattcgcg gccgcttcta gag //