##gff-version 3
##sequence-region BBa_K812000 1 4113
BBa_K812000	.	promoter	2396	2424	.	+	0	ID=annotation2184358;Name=AmpR promoter
BBa_K812000	.	primer_binding_site	4033	4051	.	+	0	ID=annotation2184362;Name=Sp6 primer
BBa_K812000	.	sequence_feature	2503	2809	.	+	0	ID=annotation2184359;Name=E. coli f1replication origin
BBa_K812000	.	sequence_feature	679	1346	.	+	0	ID=annotation2184355;Name=ColE1 rep origin
BBa_K812000	.	promoter	3012	3998	.	+	0	ID=annotation2184360;Name=CMV eukaryotic promoter
BBa_K812000	.	primer_binding_site	293	312	.	+	0	ID=annotation2184353;Name=T3_promoter
BBa_K812000	.	engineered_region	1	20	.	+	0	ID=annotation2184333;Name=Biobrick suffix
BBa_K812000	.	engineered_region	4093	4113	.	+	0	ID=annotation2184363;Name=Biobrick prefix
BBa_K812000	.	primer_binding_site	26	42	.	+	0	ID=annotation2184350;Name=T7 prom (rev)
BBa_K812000	.	CDS	1494	2344	.	+	0	ID=annotation2184357;Name=Ampicillin resistance Bla gene
BBa_K812000	.	non_covalent_binding_site	243	275	.	+	0	ID=annotation2184352;Name=Downstream polylinker (from pVZ1)
BBa_K812000	.	operator	47	242	.	+	0	ID=annotation2184351;Name=SV40 Polyadenylation site
BBa_K812000	.	primer_binding_site	329	347	.	+	0	ID=annotation2184354;Name=M13 primer
BBa_K812000	.	primer_binding_site	3916	3957	.	+	0	ID=annotation2184361;Name=CMV_fwd_primer
>BBa_K812000
tactagtagcggccgctgcaggaactatagtgagtcgtattacgtagatccagacatgataagatacattgatgagttt
ggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaacca
ttataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggt
tttttaattcgcggccgcggcgccaatgcattgggcccggtacccagcttttgttccctttagtgagggttaattgcgc
gcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccgg
aagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttc
cagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct
cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt
aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgt
aaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga
ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac
cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtat
ctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttat
ccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattag
cagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtattt
ggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctg
gtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc
tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacc
tagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaat
gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat
ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta
ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcat
cgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccccc
atgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactca
tggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaac
caagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacat
agcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagat
ccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaa
aacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa
tattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatag
gggttccgcgcacatttccccgaaaagtgccacctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggt
tacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacg
ttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacc
ccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttgga
gtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattta
taagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaa
tattaacgcttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgcta
ttacgccagtcgaccatagccaattcaatatggcgtatatggactcatgccaattcaatatggtggatctggacctgtg
ccaattcaatatggcgtatatggactcgtgccaattcaatatggtggatctggaccccagccaattcaatatggcggac
ttggcaccatgccaattcaatatggcggacttggcactgtgccaactggggaggggtctacttggcacggtgccaagtt
tgaggaggggtcttggccctgtgccaagtccgccatattgaattggcatggtgccaataatggcggccatattggctat
atgccaggatcaatatataggcaatatccaatatggccctatgccaatatggctattggccaggttcaatactatgtat
tggccctatgccatatagtattccatatatgggttttcctattgacgtagatagcccctcccaatgggcggtcccatat
accatatatggggcttcctaataccgcccatagccactcccccattgacgtcaatggtctctatatatggtctttccta
ttgacgtcatatgggcggtcctattgacgtatatggcgcctcccccattgacgtcaattacggtaaatggcccgcctgg
ctcaatgcccattgacgtcaataggaccacccaccattgacgtcaatgggatggctcattgcccattcatatccgttct
cacgccccctattgacgtcaatgacggtaaatggcccacttggcagtacatcaatatctattaatagtaacttggcaag
tacattactattggaagcacgccagggtacattggcagtactcccattgacgtcaatggcggtaaatggcccgcgatgg
ctgccaagtacatccccattgacgtcaatggggaggggcaatgacgcaaatgggcgttccattgacgtaaatgggcggt
aggcgtgcctaatgggaggtctatataagcaatgctcgtttagggaaccgccattctgcctggggacgtcggagcaagc
ttgatttaggtgacactatagaatacaagctacttgttctttttgcaggatcccatcgattcgaattcgcggccgcttc
tagag