LOCUS       BBa_K812012             1870 bp    DNA     linear       22-Dec-2024
DEFINITION  OsTirI Ubiquitinase E3 for AID tagged protein degradation in the 
            presence of auxin
ACCESSION   BBa_K812012
VERSION     BBa_K812012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..7
                     /label=kozac
     CDS             8..1732
                     /label=OsTirI
     CDS             1757..1792
                     /label=Myc
     CDS             1793..1828
                     /label=Myc
     CDS             1829..1864
                     /label=Myc
     misc_feature    1066..1066
                     /label=PstI eliminated
     misc_feature    1156..1156
                     /label=SpeI elimitated
ORIGIN
        1 agccaccatg acgtacttcc cggaggaggt ggtggagcac atcttcagct tcctgccggc
       61 gcagcgcgac cgcaacacgg tctcgctcgt ctgcaaggtg tggtacgaga tcgagaggct
      121 gagccgccgc ggcgtcttcg tgggcaactg ctacgccgtg cgcgccggcc gcgtcgccgc
      181 gcggttcccc aacgtgcggg cgctcacggt gaaggggaag ccccacttcg ccgacttcaa
      241 cctcgtgccc cccgactggg gcggctacgc ggggccgtgg atcgaggcgg ccgcgagggg
      301 atgccacggc ctggaggagc tcaggatgaa gcggatggtg gtgtccgacg agagcctcga
      361 gctgctggct cgctcgttcc cgcggttcag ggctcttgtt cttatcagct gcgaggggtt
      421 cagcactgac gggctagccg ccgtcgcgag ccattgcaag cttctgaggg agttggattt
      481 gcaggaaaat gaagtggagg atcgagggcc taggtggctt tcctgcttcc ctgattcctg
      541 cacatcactt gtctcattga attttgcctg catcaaaggg gaggttaatg ctggttcact
      601 ggagagactt gttagcaggt ccccaaacct gcggagtttg aggctgaatc gatctgtatc
      661 ggtagataca cttgcaaaga tactactgcg tacccctaac ttggaggatt tggggacagg
      721 gaatttgaca gatgacttcc aaactgagtc ctactttaag cttaccagtg ctctggagaa
      781 atgcaagatg ttgaggagtt tgtctggatt ctgggatgct tctcctgttt gcctgtcatt
      841 tatctacccc ctgtgtgctc aactgacagg attgaacttg agctatgccc ccacacttga
      901 tgcttctgac cttacaaaaa tgattagccg ctgtgtgaag ctccaacgcc tttgggtact
      961 ggattgtatc tcggacaaag gcttgcaagt ggtggcctcc agttgcaaag acttgcaaga
     1021 actcagggta tttccatcag atttctacgt agctggttat tctgctgtga cagaggaggg
     1081 acttgttgca gtatccttgg gctgtccaaa actgaactca ctactgtact tctgtcacca
     1141 aatgactaat gctgctctag ttactgtcgc caagaactgt ccaaatttca cacgattcag
     1201 actttgtatt cttgagccag ggaagcctga tgttgtgaca agccaaccat tagatgaagg
     1261 ctttggagct attgttcgtg agtgcaaggg attacaacgt ttgtcaatat ctggtcttct
     1321 cacagacaaa gttttcatgt atattgggaa atatgcaaaa caacttgaga tgctttctat
     1381 agcatttgct ggtgacagtg ataagggtat gatgcatgtt atgaatggat gcaagaattt
     1441 aaggaaactg gagataagag atagcccgtt tggtgatgct gcactcttgg ggaattttgc
     1501 taggtacgag acaatgcgat ccctttggat gtcatcttgc aatgtcacgt taaaggggtg
     1561 ccaagtcctt gcgtcaaaga tgccgatgct caatgttgag gtcataaatg agcgggatgg
     1621 tagcaatgaa atggaggaaa accatggaga tctgcctaaa gtggagaaat tatatgtgta
     1681 ccgcacaact gctggggcga gggatgatgc accaaatttt gttaaaatcc tagtcgactc
     1741 cggttctgct gctagtggtg aacaaaagtt gatttctgaa gaagatttga acggggaaca
     1801 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
     1861 gaactaataa
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