LOCUS       BBa_K812013             3427 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP-AID OsTirI polysistronic system for auxin detection in tadpole
ACCESSION   BBa_K812013
VERSION     BBa_K812013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..7
                     /label=kozac
     CDS             8..1501
                     /label=GFP-AID (K81010)
     misc_feature    1502..1564
                     /label=Pep2A (self cleavable peptide)
     CDS             1565..3421
                     /label=OsTirI (K81011)
ORIGIN
        1 agccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      481 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      721 caagggagct ggtgcaggcg ctggagcggg tgccgggccc atgatgggca gtgtcgagct
      781 gaatctgagg gagactgagc tgtgtcttgg tcttcccggt ggagatacag tggctccggt
      841 aaccggaaac aagagagggt tctcagagac ggttgatctg aagctaaatc tgaataatga
      901 gcctgcaaac aaggaaggat ctacgactca tgacgtcgtg acttttgatt ccaaggagaa
      961 gagtgcttgt cctaaagatc cagccaaacc tccggccaag gcacaagttg tgggatggcc
     1021 accggtgaga tcataccgga agaacgtgat ggtttcctgc caaaaatcaa gcggtggccc
     1081 ggaggcggcg gcgttcgtga aggtatcaat ggacggagca ccgtacttga ggaaaatcga
     1141 tttgaggatg tataaaagct acgatgagct ttctaatgct ttgtccaaca tgttcagctc
     1201 ttttaccatg ggcaaacatg gaggagaaga aggaatgata gacttcatga atgagaggaa
     1261 attgatggat ttggtgaata gctgggacta tgttccctct tatgaagaca aagacggtga
     1321 ttggatgctc gtcggcgacg ttccttggcc aatgttcgtc gatacatgca agcgtttacg
     1381 tctcatgaaa ggatcggatg ccattggtct cgctccgagg gcgatggaga agtgcaagag
     1441 cagagctggt gctggcgccc ccaagaagaa aagaaaggtc cctaaaaaga aacgtaaggt
     1501 tggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     1561 cccaatgacg tacttcccgg aggaggtggt ggagcacatc ttcagcttcc tgccggcgca
     1621 gcgcgaccgc aacacggtct cgctcgtctg caaggtgtgg tacgagatcg agaggctgag
     1681 ccgccgcggc gtcttcgtgg gcaactgcta cgccgtgcgc gccggccgcg tcgccgcgcg
     1741 gttccccaac gtgcgggcgc tcacggtgaa ggggaagccc cacttcgccg acttcaacct
     1801 cgtgcccccc gactggggcg gctacgcggg gccgtggatc gaggcggccg cgaggggatg
     1861 ccacggcctg gaggagctca ggatgaagcg gatggtggtg tccgacgaga gcctcgagct
     1921 gctggctcgc tcgttcccgc ggttcagggc tcttgttctt atcagctgcg aggggttcag
     1981 cactgacggg ctagccgccg tcgcgagcca ttgcaagctt ctgagggagt tggatttgca
     2041 ggaaaatgaa gtggaggatc gagggcctag gtggctttcc tgcttccctg attcctgcac
     2101 atcacttgtc tcattgaatt ttgcctgcat caaaggggag gttaatgctg gttcactgga
     2161 gagacttgtt agcaggtccc caaacctgcg gagtttgagg ctgaatcgat ctgtatcggt
     2221 agatacactt gcaaagatac tactgcgtac ccctaacttg gaggatttgg ggacagggaa
     2281 tttgacagat gacttccaaa ctgagtccta ctttaagctt accagtgctc tggagaaatg
     2341 caagatgttg aggagtttgt ctggattctg ggatgcttct cctgtttgcc tgtcatttat
     2401 ctaccccctg tgtgctcaac tgacaggatt gaacttgagc tatgccccca cacttgatgc
     2461 ttctgacctt acaaaaatga ttagccgctg tgtgaagctc caacgccttt gggtactgga
     2521 ttgtatctcg gacaaaggct tgcaagtggt ggcctccagt tgcaaagact tgcaagaact
     2581 cagggtattt ccatcagatt tctacgtagc tggttattct gctgtgacag aggagggact
     2641 tgttgcagta tccttgggct gtccaaaact gaactcacta ctgtacttct gtcaccaaat
     2701 gactaatgct gctctagtta ctgtcgccaa gaactgtcca aatttcacac gattcagact
     2761 ttgtattctt gagccaggga agcctgatgt tgtgacaagc caaccattag atgaaggctt
     2821 tggagctatt gttcgtgagt gcaagggatt acaacgtttg tcaatatctg gtcttctcac
     2881 agacaaagtt ttcatgtata ttgggaaata tgcaaaacaa cttgagatgc tttctatagc
     2941 atttgctggt gacagtgata agggtatgat gcatgttatg aatggatgca agaatttaag
     3001 gaaactggag ataagagata gcccgtttgg tgatgctgca ctcttgggga attttgctag
     3061 gtacgagaca atgcgatccc tttggatgtc atcttgcaat gtcacgttaa aggggtgcca
     3121 agtccttgcg tcaaagatgc cgatgctcaa tgttgaggtc ataaatgagc gggatggtag
     3181 caatgaaatg gaggaaaacc atggagatct gcctaaagtg gagaaattat atgtgtaccg
     3241 cacaactgct ggggcgaggg atgatgcacc aaattttgtt aaaatcctag tcgactccgg
     3301 ttctgctgct agtggtgaac aaaagttgat ttctgaagaa gatttgaacg gggaacaaaa
     3361 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     3421 ctaataa
//