LOCUS       BBa_K812051             2217 bp    DNA     linear       22-Dec-2024
DEFINITION  Golden brick version of pSB1K3 plasmid for Golden Gate and Golden 
            Brick cloning
ACCESSION   BBa_K812051
VERSION     BBa_K812051.1  
FEATURES             Location/Qualifiers
     misc_feature    1..27
                     /label=Goldenbrick suffix
     misc_feature    1..6
                     /label=SpeI
     misc_feature    7..12
                     /label=BsaI
     misc_feature    14..17
                     /label=OH5
     misc_feature    17..22
                     /label=BbsI
     misc_feature    23..27
                     /label=PstI
     stem_loop       29..100
                     /label=E. coli his Operon terminator
     primer_bind     164..183
                     /label=VR
     misc_feature    268..882
                     /label=pMB1 rep origin
     misc_feature    1104..1919
                     /label=Kanmycin resistance marker CDS
     misc_feature    2024..2067
                     /label=Repeat region
     primer_bind     2074..2093
                     /label=VF2
     stem_loop       2163..2182
                     /label=Terminator
     misc_feature    2191..2217
                     /label=Goldenbrick prefix
     misc_feature    2191..2195
                     /label=EcoRI
     misc_feature    2196..2201
                     /label=BbsI
     misc_feature    2201..2204
                     /label=OH1
     misc_feature    2206..2211
                     /label=BsaI
     misc_feature    2212..2217
                     /label=XbaI
ORIGIN
        1 actagtggtc tccagcgtct tcctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
       61 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagc tcgagtcccg tcaagtcagc gtaatgctct gccagtgtta
     1081 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     1141 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     1201 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     1261 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     1321 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt
     1381 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     1441 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     1501 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     1561 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     1621 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     1681 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     1741 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     1801 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     1861 catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac
     1921 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     1981 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     2041 aacttttgct gagttgaagg atcagctcga gtgccacctg acgtctaaga aaccattatt
     2101 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2161 tccttagctt tcgctaagga tgatttctgg aattcgaaga cgcttgagac ctctaga
//