##gff-version 3
##sequence-region BBa_K812051 1 2217
BBa_K812051	.	primer_binding_site	2074	2093	.	+	0	ID=annotation2195396;Name=VF2
BBa_K812051	.	sequence_feature	2196	2201	.	+	0	ID=annotation2195400;Name=BbsI
BBa_K812051	.	sequence_feature	23	27	.	+	0	ID=annotation2195383;Name=PstI
BBa_K812051	.	engineered_region	1	27	.	+	0	ID=annotation2195378;Name=Goldenbrick suffix
BBa_K812051	.	primer_binding_site	164	183	.	+	0	ID=annotation2195385;Name=VR
BBa_K812051	.	sequence_feature	1	6	.	+	0	ID=annotation2195379;Name=SpeI
BBa_K812051	.	sequence_feature	2191	2195	.	+	0	ID=annotation2195399;Name=EcoRI
BBa_K812051	.	sequence_feature	2206	2211	.	+	0	ID=annotation2195402;Name=BsaI
BBa_K812051	.	sequence_feature	1104	1919	.	+	0	ID=annotation2195394;Name=Kanmycin resistance marker CDS
BBa_K812051	.	sequence_feature	2024	2067	.	+	0	ID=annotation2195395;Name=Repeat region
BBa_K812051	.	stem_loop	29	100	.	+	0	ID=annotation2195384;Name=E. coli his Operon terminator
BBa_K812051	.	sequence_feature	14	17	.	+	0	ID=annotation2195381;Name=OH5
BBa_K812051	.	stem_loop	2163	2182	.	+	0	ID=annotation2195397;Name=Terminator
BBa_K812051	.	sequence_feature	2201	2204	.	+	0	ID=annotation2195401;Name=OH1
BBa_K812051	.	engineered_region	2191	2217	.	+	0	ID=annotation2195398;Name=Goldenbrick prefix
BBa_K812051	.	sequence_feature	7	12	.	+	0	ID=annotation2195380;Name=BsaI
BBa_K812051	.	sequence_feature	268	882	.	+	0	ID=annotation2195393;Name=pMB1 rep origin
BBa_K812051	.	sequence_feature	17	22	.	+	0	ID=annotation2195382;Name=BbsI
BBa_K812051	.	sequence_feature	2212	2217	.	+	0	ID=annotation2195408;Name=XbaI
>BBa_K812051
actagtggtctccagcgtcttcctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaacc
gaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta
tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcc
agcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaa
aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctc
gtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctc
atagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca
gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagca
gccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct
acactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaa
gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagat
tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaac
ttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattaga
aaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgttt
ctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgt
ccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactg
aatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaa
atcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaagga
caattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggat
attcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaa
atgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacg
ctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcc
cgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgt
ttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatata
tttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagt
tgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatc
acgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgaagacgcttgagacct
ctaga