BBa_K812051 1 BBa_K812051 Golden brick version of pSB1K3 plasmid for Golden Gate and Golden Brick cloning 2012-09-23T11:00:00Z 2015-05-08T01:13:27Z This part is a derivation of the pSB1K3 plasmid from the registry. This is a modified version of pSB1K3 from the registry with the J4450 negative control. The biobrick sites are modified to incorporate a BsaI restriction site that enables the use of golden gate cloning and golden brick cloning to assemble full contructs in a single shot of digestion/ligation. The complete protocol of the goldengate can be find in the original paper: Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes. PLoS ONE 4(5): e5553. doi:10.1371/journal.pone.0005553 The Golden Brick assembly is an invention of the iGEM Evry 2012 Team. A complete description of the procedure can be find on our website: http://2012.igem.org/Team:Evry/GB false false _1069_ 0 8998 9 Not in stock false The negative control J4450 has been amplified with new goldenbrick extensions using PCR and re-inserted in pSB1C3 using EcoRI and PstI cloning. false Cyrille Pauthenier annotation2195401 1 OH1 range2195401 1 2201 2204 annotation2195399 1 EcoRI range2195399 1 2191 2195 annotation2195393 1 pMB1 rep origin range2195393 1 268 882 annotation2195383 1 PstI range2195383 1 23 27 annotation2195379 1 SpeI range2195379 1 1 6 annotation2195382 1 BbsI range2195382 1 17 22 annotation2195395 1 Repeat region range2195395 1 2024 2067 annotation2195397 1 Terminator range2195397 1 2163 2182 annotation2195408 1 XbaI range2195408 1 2212 2217 annotation2195396 1 VF2 range2195396 1 2074 2093 annotation2195398 1 Goldenbrick prefix range2195398 1 2191 2217 annotation2195400 1 BbsI range2195400 1 2196 2201 annotation2195381 1 OH5 range2195381 1 14 17 annotation2195384 1 E. coli his Operon terminator range2195384 1 29 100 annotation2195380 1 BsaI range2195380 1 7 12 annotation2195402 1 BsaI range2195402 1 2206 2211 annotation2195385 1 VR range2195385 1 164 183 annotation2195394 1 Kanmycin resistance marker CDS range2195394 1 1104 1919 annotation2195378 1 Goldenbrick suffix range2195378 1 1 27 BBa_K812051_sequence 1 actagtggtctccagcgtcttcctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgaagacgcttgagacctctaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z