BBa_K812054
1
BBa_K812054
RFP E1010 with GoldenBrick sequences
2012-09-19T11:00:00Z
2015-05-08T01:13:27Z
The part E1010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.
RFP E1010 with GoldenBrick extension
This part is the GoldenBricked version of E1010 RFP. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.
To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB
false
false
_1069_
0
8998
9
It's complicated
false
This part has been created from E1010 by PCR amplification
false
Cyrille Pauthenier
annotation2201529
1
GoldenBrick mRFP1 prefix
range2201529
1
1
17
annotation2201527
1
GoldenBrick mRFP1 suffix
range2201527
1
699
722
annotation2201785
1
XbaI
range2201785
1
13
17
annotation2201782
1
EcoRI
range2201782
1
1
6
annotation2201786
1
BbsI
range2201786
1
699
704
annotation2201791
1
SpeI
range2201791
1
712
717
annotation2201789
1
BsaI
range2201789
1
706
711
annotation2201781
1
OH4
range2201781
1
701
704
annotation2201530
1
OH3
range2201530
1
14
17
annotation2201783
1
BsaI
range2201783
1
7
12
annotation2201792
1
PstI
range2201792
1
718
722
annotation2201528
1
mRFP1
range2201528
1
18
692
BBa_K812054_sequence
1
gaattcggtctctctagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagaagacagagaccactagtctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z