BBa_K812054 1 BBa_K812054 RFP E1010 with GoldenBrick sequences 2012-09-19T11:00:00Z 2015-05-08T01:13:27Z The part E1010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI. RFP E1010 with GoldenBrick extension This part is the GoldenBricked version of E1010 RFP. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed. To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB false false _1069_ 0 8998 9 It's complicated false This part has been created from E1010 by PCR amplification false Cyrille Pauthenier annotation2201527 1 GoldenBrick mRFP1 suffix range2201527 1 699 722 annotation2201789 1 BsaI range2201789 1 706 711 annotation2201783 1 BsaI range2201783 1 7 12 annotation2201530 1 OH3 range2201530 1 14 17 annotation2201792 1 PstI range2201792 1 718 722 annotation2201785 1 XbaI range2201785 1 13 17 annotation2201528 1 mRFP1 range2201528 1 18 692 annotation2201529 1 GoldenBrick mRFP1 prefix range2201529 1 1 17 annotation2201782 1 EcoRI range2201782 1 1 6 annotation2201781 1 OH4 range2201781 1 701 704 annotation2201791 1 SpeI range2201791 1 712 717 annotation2201786 1 BbsI range2201786 1 699 704 BBa_K812054_sequence 1 gaattcggtctctctagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataagaagacagagaccactagtctgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z