LOCUS       BBa_K812010             1503 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP fused with AID-tag  (ubuquitinase E3 OsTirI recoginition 
            domain)
ACCESSION   BBa_K812010
VERSION     BBa_K812010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Kozac
     CDS             7..723
                     /label=GFP
     CDS             724..756
                     /label=unfolded linker
     CDS             757..1443
                     /label=AID degradation tag
     CDS             1459..1479
                     /label=NLS1
     CDS             1480..1500
                     /label=NLS2
ORIGIN
        1 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagggagctg gtgcaggcgc tggagcgggt gccgggccca tgatgggcag tgtcgagctg
      781 aatctgaggg agactgagct gtgtcttggt cttcccggtg gagatacagt ggctccggta
      841 accggaaaca agagagggtt ctcagagacg gttgatctga agctaaatct gaataatgag
      901 cctgcaaaca aggaaggatc tacgactcat gacgtcgtga cttttgattc caaggagaag
      961 agtgcttgtc ctaaagatcc agccaaacct ccggccaagg cacaagttgt gggatggcca
     1021 ccggtgagat cataccggaa gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg
     1081 gaggcggcgg cgttcgtgaa ggtatcaatg gacggagcac cgtacttgag gaaaatcgat
     1141 ttgaggatgt ataaaagcta cgatgagctt tctaatgctt tgtccaacat gttcagctct
     1201 tttaccatgg gcaaacatgg aggagaagaa ggaatgatag acttcatgaa tgagaggaaa
     1261 ttgatggatt tggtgaatag ctgggactat gttccctctt atgaagacaa agacggtgat
     1321 tggatgctcg tcggcgacgt tccttggcca atgttcgtcg atacatgcaa gcgtttacgt
     1381 ctcatgaaag gatcggatgc cattggtctc gctccgaggg cgatggagaa gtgcaagagc
     1441 agagctggtg ctggcgcccc caagaagaaa agaaaggtcc ctaaaaagaa acgtaaggtt
     1501 taa
//
LOCUS       BBa_K812110             1503 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for 
            PSC2+ plasmid
ACCESSION   BBa_K812110
VERSION     BBa_K812110.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Kozac
     CDS             7..723
                     /label=GFP
     CDS             724..756
                     /label=unfolded linker
     CDS             757..1443
                     /label=AID degradation tag
     CDS             1444..1479
                     /label=NLS1
     CDS             1480..1500
                     /label=NLS2
ORIGIN
        1 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagggagctg gtgcaggcgc tggagcgggt gccgggccca tgatgggcag tgtcgagctg
      781 aatctgaggg agactgagct gtgtcttggt cttcccggtg gagatacagt ggctccggta
      841 accggaaaca agagagggtt ctcagagacg gttgatctga agctaaatct gaataatgag
      901 cctgcaaaca aggaaggatc tacgactcat gacgtcgtga cttttgattc caaggagaag
      961 agtgcttgtc ctaaagatcc agccaaacct ccggccaagg cacaagttgt gggatggcca
     1021 ccggtgagat cataccggaa gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg
     1081 gaggcggcgg cgttcgtgaa ggtatcaatg gacggagcac cgtacttgag gaaaatcgat
     1141 ttgaggatgt ataaaagcta cgatgagctt tctaatgctt tgtccaacat gttcagctct
     1201 tttaccatgg gcaaacatgg aggagaagaa ggaatgatag acttcatgaa tgagaggaaa
     1261 ttgatggatt tggtgaatag ctgggactat gttccctctt atgaagacaa agacggtgat
     1321 tggatgctcg tcggcgacgt tccttggcca atgttcgtcg atacatgcaa gcgtttacgt
     1381 ctcatgaaag gatcggatgc cattggtctc gctccgaggg cgatggagaa gtgcaagagc
     1441 agagctggtg ctggcgcccc caagaagaaa agaaaggtcc ctaaaaagaa acgtaaggtt
     1501 taa
//