BBa_K812110 1 GFP-AID GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid 2012-09-19T11:00:00Z 2015-05-08T01:13:27Z The source plasmid was provided in K. Nishimura, T. Fukagawa, H. Takisawa, T. Kakimoto, and M. Kane- maki. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature methods, 6(12) :917???922, 2009. Released HQ 2013 This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken. The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration. This part can be used in an oviparian plasmid such as pSC2+ (see K812000) true false _1069_ 0 14045 9 Discontinued false This part has been biobricked using PCR method and cloned in pSB1C3. false Pierre Parutto annotation2187957 1 GFP range2187957 1 7 723 annotation2187959 1 AID degradation tag range2187959 1 757 1443 annotation2187961 1 NLS2 range2187961 1 1480 1500 annotation2187958 1 unfolded linker range2187958 1 724 756 annotation2187960 1 NLS1 range2187960 1 1444 1479 annotation2187956 1 Kozac range2187956 1 1 6 BBa_K812010 1 GFP-AID GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain) 2012-09-17T11:00:00Z 2015-05-08T01:13:27Z This part have been extracted by PCR from the plasmid pNHK60 obtained from the riken registry. Released HQ 2013 This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken. The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration. false true _1069_ 0 8998 9 In stock false This part has been biobricked using PCR method and cloned in pSB1C3 false Cyrille Pauthenier annotation2184374 1 NLS2 range2184374 1 1480 1500 annotation2184372 1 AID degradation tag range2184372 1 757 1443 annotation2184371 1 unfolded linker range2184371 1 724 756 annotation2184373 1 NLS1 range2184373 1 1459 1479 annotation2184369 1 Kozac range2184369 1 1 6 annotation2184370 1 GFP range2184370 1 7 723 BBa_K812010_sequence 1 gccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggagctggtgcaggcgctggagcgggtgccgggcccatgatgggcagtgtcgagctgaatctgagggagactgagctgtgtcttggtcttcccggtggagatacagtggctccggtaaccggaaacaagagagggttctcagagacggttgatctgaagctaaatctgaataatgagcctgcaaacaaggaaggatctacgactcatgacgtcgtgacttttgattccaaggagaagagtgcttgtcctaaagatccagccaaacctccggccaaggcacaagttgtgggatggccaccggtgagatcataccggaagaacgtgatggtttcctgccaaaaatcaagcggtggcccggaggcggcggcgttcgtgaaggtatcaatggacggagcaccgtacttgaggaaaatcgatttgaggatgtataaaagctacgatgagctttctaatgctttgtccaacatgttcagctcttttaccatgggcaaacatggaggagaagaaggaatgatagacttcatgaatgagaggaaattgatggatttggtgaatagctgggactatgttccctcttatgaagacaaagacggtgattggatgctcgtcggcgacgttccttggccaatgttcgtcgatacatgcaagcgtttacgtctcatgaaaggatcggatgccattggtctcgctccgagggcgatggagaagtgcaagagcagagctggtgctggcgcccccaagaagaaaagaaaggtccctaaaaagaaacgtaaggtttaa BBa_K812110_sequence 1 gccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagggagctggtgcaggcgctggagcgggtgccgggcccatgatgggcagtgtcgagctgaatctgagggagactgagctgtgtcttggtcttcccggtggagatacagtggctccggtaaccggaaacaagagagggttctcagagacggttgatctgaagctaaatctgaataatgagcctgcaaacaaggaaggatctacgactcatgacgtcgtgacttttgattccaaggagaagagtgcttgtcctaaagatccagccaaacctccggccaaggcacaagttgtgggatggccaccggtgagatcataccggaagaacgtgatggtttcctgccaaaaatcaagcggtggcccggaggcggcggcgttcgtgaaggtatcaatggacggagcaccgtacttgaggaaaatcgatttgaggatgtataaaagctacgatgagctttctaatgctttgtccaacatgttcagctcttttaccatgggcaaacatggaggagaagaaggaatgatagacttcatgaatgagaggaaattgatggatttggtgaatagctgggactatgttccctcttatgaagacaaagacggtgattggatgctcgtcggcgacgttccttggccaatgttcgtcgatacatgcaagcgtttacgtctcatgaaaggatcggatgccattggtctcgctccgagggcgatggagaagtgcaagagcagagctggtgctggcgcccccaagaagaaaagaaaggtccctaaaaagaaacgtaaggtttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z