##gff-version 3
##sequence-region BBa_K812200 1 3312
BBa_K812200	.	promoter	2396	2424	.	+	0	ID=annotation2188505;Name=AmpR promoter
BBa_K812200	.	non_covalent_binding_site	243	275	.	+	0	ID=annotation2188494;Name=Downstream polylinker (from pVZ1)
BBa_K812200	.	sequence_feature	679	1346	.	+	0	ID=annotation2188502;Name=ColE1 rep origin
BBa_K812200	.	primer_binding_site	293	312	.	+	0	ID=annotation2188495;Name=T3_promoter
BBa_K812200	.	engineered_region	3292	3312	.	+	0	ID=annotation2188514;Name=BioBrick prefix
BBa_K812200	.	primer_binding_site	329	347	.	+	0	ID=annotation2188496;Name=M13 primer 
BBa_K812200	.	engineered_region	1	20	.	+	0	ID=annotation2188490;Name=BioBrick suffix
BBa_K812200	.	primer_binding_site	26	42	.	+	0	ID=annotation2188491;Name=T7 prom
BBa_K812200	.	sequence_feature	2503	2809	.	+	0	ID=annotation2188507;Name=E.coli f1replication origin
BBa_K812200	.	CDS	1494	2344	.	+	0	ID=annotation2188503;Name=Ampicillin resistance Bla gene
BBa_K812200	.	promoter	3012	3224	.	+	0	ID=annotation2188509;Name=Elastase
BBa_K812200	.	operator	47	242	.	+	0	ID=annotation2188493;Name=SV40 Polyadenylation site
BBa_K812200	.	primer_binding_site	3232	3249	.	+	0	ID=annotation2188513;Name=Sp6 primer
>BBa_K812200
tactagtagcggccgctgcaggaactatagtgagtcgtattacgtagatccagacatgataagatacattgatgagttt
ggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaacca
ttataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggt
tttttaattcgcggccgcggcgccaatgcattgggcccggtacccagcttttgttccctttagtgagggttaattgcgc
gcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccgg
aagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttc
cagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct
cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt
aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgt
aaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga
ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac
cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtat
ctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttat
ccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattag
cagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtattt
ggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctg
gtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc
tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacc
tagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaat
gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat
ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta
ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcat
cgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccccc
atgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactca
tggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaac
caagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacat
agcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagat
ccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaa
aacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa
tattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatag
gggttccgcgcacatttccccgaaaagtgccacctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggt
tacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacg
ttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacc
ccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttgga
gtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattta
taagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaa
tattaacgcttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgcta
ttacgccagtcgacttgggttaactgagtgccggccttgttctgtctttgaatatcagataaatgagttgacttaaaat
ttgttcatttgtactttcatgtcacctgtgcttttccctgccttctacccacagccctgcccagctggcaggaggaagg
tcagcagagctgctgataagagccgtataaagagggttccgctcatggcaaggggcagtggtctaagcttgatttaggt
gacactatagaatacaagctacttgttctttttgcaggatcccatcgattcgaattcgcggccgcttctagag