LOCUS       BBa_K812300             3591 bp    DNA     linear       29-May-2024
DEFINITION  Biobricked pCS2+ plasmid with HSP70 promoter for frogs and chicken
ACCESSION   BBa_K812300
VERSION     BBa_K812300.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label= Biobrick suffix
     primer_bind     26..42
                     /label=T7 promoter (rev)
     misc_feature    47..242
                     /label=SV40 Polyadenylation site
     misc_feature    243..275
                     /label=downstream polylinker (from pVZ1) 
     primer_bind     293..312
                     /label=T3_promoter
     primer_bind     329..347
                     /label=M13 primer
     misc_feature    679..1346
                     /label=ColE1 rep origin
     CDS             1494..2344
                     /label=Ampicillin resistant Bla gene
     promoter        2396..2424
                     /label=AmpR promoter
     misc_feature    2503..2809
                     /label=E. coli f1 replication origin 
     promoter        3017..3503
                     /label=HSP70
     misc_feature    3511..3528
                     /label=SP6 primer
     misc_feature    3571..3591
                     /label=prefix
ORIGIN
        1 tactagtagc ggccgctgca ggaactatag tgagtcgtat tacgtagatc cagacatgat
       61 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      121 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      181 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      241 ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag
      301 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2461 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2521 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2581 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2641 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2701 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2761 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2821 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2881 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3001 tattacgcca gtcgaccccg tttagcagga aatagccttg ggttgatggg aaggaatgga
     3061 catggtcaga gacaagacag aagtacagag gaacgcatat ctatttcaca tgagaaaacg
     3121 actctctcga gaaagctcgc gaatcttccg cgattgtgac taactcagca accgtgacac
     3181 tgccggaaac ctcgcgaaag ttcttcgggt gatctcatac gcaaaactgg ttgctgattg
     3241 gctaacgaaa tggaagcctc gggaaacttc gggtcggttg ctaaggtgac cgtggctggc
     3301 gtggcgtgta ctgtgccttg attggctaat ctgttctcag agttctgtat aaatacagcg
     3361 ggtttgaagc gatgtggaag tagcagaatc taagctgaca cttgtgagat ctgacagaga
     3421 agagcaactg cacagcgata cttactgggc aaagacgcag ctgcgcatat tctagcgaaa
     3481 agcaaactgt aaggagcgca aataagcttg atttaggtga cactatagaa tacaagctac
     3541 ttgttctttt tgcaggatcc catcgattcg aattcgcggc cgcttctaga g
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