BBa_K812300 1 BBa_K812300 Biobricked pCS2+ plasmid with HSP70 promoter for frogs and chicken 2012-09-19T11:00:00Z 2015-05-08T01:13:28Z The plasmid template is from K812000, The constitutive CMV promoter was replaced by Heat Shock Promoter 70, and sfGFP is from K812031. HSP70 is from: Love, N.R. et al., 2011. pTransgenesis: a cross-species, modular transgenesis resource. Development (Cambridge, England), 138(24), pp.5451???8 This plasmid is a biobricked version of the plasmid pSC2+(K812000)with HSP70 promoter. This plasmid is made of a bacterial ampicilin resistant backbone. The Biobrick site is surrounded by a 5'UTR expression enhancer, and a poly-A tail with a SV40 3' UTR that enhance the stabilisation of the mRNA and the expression of the protein. To use this plasmid, you simply have to insert your protein between the EcoRI and PstI site. It is better to add a kozac sequence in front of your gene to enhance its expression. This plasmid contains the standard registry insert J04450 as a negative cloning control. false false _1069_ 0 12000 9 Not in stock false CMV of pCS2+ (K812000) was replaced by HSP70 using the double digestion SalI and HindIII. Then J04450 was replaced by sfGFP using the Biobrick K812031 with the double digestion EcoRI and PstI. false Tristan Cerisy annotation2188489 1 Biobrick suffix range2188489 1 1 20 annotation2188511 1 SP6 primer range2188511 1 3511 3528 annotation2188492 1 T7 promoter (rev) range2188492 1 26 42 annotation2188500 1 M13 primer range2188500 1 329 347 annotation2188504 1 Ampicillin resistant Bla gene range2188504 1 1494 2344 annotation2188508 1 E. coli f1 replication origin range2188508 1 2503 2809 annotation2188510 1 HSP70 range2188510 1 3017 3503 annotation2188506 1 AmpR promoter range2188506 1 2396 2424 annotation2188501 1 ColE1 rep origin range2188501 1 679 1346 annotation2188497 1 SV40 Polyadenylation site range2188497 1 47 242 annotation2188515 1 prefix range2188515 1 3571 3591 annotation2188499 1 T3_promoter range2188499 1 293 312 annotation2188498 1 downstream polylinker (from pVZ1) range2188498 1 243 275 BBa_K812300_sequence 1 tactagtagcggccgctgcaggaactatagtgagtcgtattacgtagatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaattcgcggccgcggcgccaatgcattgggcccggtacccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagtcgaccccgtttagcaggaaatagccttgggttgatgggaaggaatggacatggtcagagacaagacagaagtacagaggaacgcatatctatttcacatgagaaaacgactctctcgagaaagctcgcgaatcttccgcgattgtgactaactcagcaaccgtgacactgccggaaacctcgcgaaagttcttcgggtgatctcatacgcaaaactggttgctgattggctaacgaaatggaagcctcgggaaacttcgggtcggttgctaaggtgaccgtggctggcgtggcgtgtactgtgccttgattggctaatctgttctcagagttctgtataaatacagcgggtttgaagcgatgtggaagtagcagaatctaagctgacacttgtgagatctgacagagaagagcaactgcacagcgatacttactgggcaaagacgcagctgcgcatattctagcgaaaagcaaactgtaaggagcgcaaataagcttgatttaggtgacactatagaatacaagctacttgttctttttgcaggatcccatcgattcgaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z