##gff-version 3
##sequence-region BBa_K812300 1 3591
BBa_K812300	.	engineered_region	1	20	.	+	0	ID=annotation2188489;Name= Biobrick suffix
BBa_K812300	.	primer_binding_site	329	347	.	+	0	ID=annotation2188500;Name=M13 primer
BBa_K812300	.	non_covalent_binding_site	3511	3528	.	+	0	ID=annotation2188511;Name=SP6 primer
BBa_K812300	.	sequence_feature	679	1346	.	+	0	ID=annotation2188501;Name=ColE1 rep origin
BBa_K812300	.	primer_binding_site	26	42	.	+	0	ID=annotation2188492;Name=T7 promoter (rev)
BBa_K812300	.	engineered_region	3571	3591	.	+	0	ID=annotation2188515;Name=prefix
BBa_K812300	.	CDS	1494	2344	.	+	0	ID=annotation2188504;Name=Ampicillin resistant Bla gene
BBa_K812300	.	promoter	3017	3503	.	+	0	ID=annotation2188510;Name=HSP70
BBa_K812300	.	promoter	2396	2424	.	+	0	ID=annotation2188506;Name=AmpR promoter
BBa_K812300	.	sequence_feature	2503	2809	.	+	0	ID=annotation2188508;Name=E. coli f1 replication origin 
BBa_K812300	.	primer_binding_site	293	312	.	+	0	ID=annotation2188499;Name=T3_promoter
BBa_K812300	.	non_covalent_binding_site	243	275	.	+	0	ID=annotation2188498;Name=downstream polylinker (from pVZ1) 
BBa_K812300	.	operator	47	242	.	+	0	ID=annotation2188497;Name=SV40 Polyadenylation site
>BBa_K812300
tactagtagcggccgctgcaggaactatagtgagtcgtattacgtagatccagacatgataagatacattgatgagttt
ggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaacca
ttataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggt
tttttaattcgcggccgcggcgccaatgcattgggcccggtacccagcttttgttccctttagtgagggttaattgcgc
gcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccgg
aagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttc
cagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct
cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt
aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgt
aaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga
ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac
cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtat
ctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttat
ccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattag
cagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtattt
ggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctg
gtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc
tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacc
tagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaat
gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat
ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta
ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcat
cgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccccc
atgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactca
tggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaac
caagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacat
agcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagat
ccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaa
aacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa
tattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatag
gggttccgcgcacatttccccgaaaagtgccacctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggt
tacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacg
ttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacc
ccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttgga
gtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattta
taagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaa
tattaacgcttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgcta
ttacgccagtcgaccccgtttagcaggaaatagccttgggttgatgggaaggaatggacatggtcagagacaagacaga
agtacagaggaacgcatatctatttcacatgagaaaacgactctctcgagaaagctcgcgaatcttccgcgattgtgac
taactcagcaaccgtgacactgccggaaacctcgcgaaagttcttcgggtgatctcatacgcaaaactggttgctgatt
ggctaacgaaatggaagcctcgggaaacttcgggtcggttgctaaggtgaccgtggctggcgtggcgtgtactgtgcct
tgattggctaatctgttctcagagttctgtataaatacagcgggtttgaagcgatgtggaagtagcagaatctaagctg
acacttgtgagatctgacagagaagagcaactgcacagcgatacttactgggcaaagacgcagctgcgcatattctagc
gaaaagcaaactgtaaggagcgcaaataagcttgatttaggtgacactatagaatacaagctacttgttctttttgcag
gatcccatcgattcgaattcgcggccgcttctagag